Design and performance analysis of multi-enzyme activity-doped nanozymes assisted by machine learning.

Traditional design approaches for nanozymes typically rely on empirical methods and trial-and-error, which hampers systematic optimization of their structure and performance, thus limiting the efficiency of developing innovative nanozymes. This study leverages machine learning techniques supported by high-throughput computations to effectively design nanozymes with multi-enzyme activities and to elucidate their reaction mechanisms. Additionally, it investigates the impact of dopants on the microphysical properties of nanozymes.

Phytoplankton Diversity, Spatial Patterns, and Photosynthetic Characteristics Under Environmental Gradients and Anthropogenic Influence in the Pearl River Estuary.

The Pearl River Estuary (PRE) is one of the world's most urbanized subtropical coastal systems. It presents a typical environmental gradient suitable for studying estuarine phytoplankton communities' dynamics and photosynthetic physiology. In September 2018, the maximum photochemical quantum yield (/) of phytoplankton in different salinity habitats of PRE (oceanic, estuarine, and freshwater zones) was studied, revealing a complex correlation with the environment.

The effects of temperature and sulfamethoxazole on the growth and photosynthetic characteristics of Phaeodactylum tricornutum.

The misuse of antibiotics has brought potential ecological risks to marine ecosystems, especially under a changing climate. Laboratory experiments were conducted to understand the impact of rising temperatures and antibiotic sulfamethoxazole (SMX) abuse on marine diatom Phaeodactylum tricornutum. Temperatures of 21 and 24 °C were optimal for the growth and photosynthetic characteristics of P.

Multienzyme-Like Nanozyme Encapsulated Ocular Microneedles for Keratitis Treatment.

Keratitis, an inflammation of the cornea caused by bacterial or fungal infections, is one of the leading causes of severe visual disability and blindness. Keratitis treatment requires both the prevention of infection and the reduction of inflammation. However, owing to their limited therapeutic functions, in addition to the ocular barrier, existing conventional medications are characterized by poor efficacy and low bioavailability, requiring high dosages or frequent topical treatment, which represents a burden on patients and increases the risk of side effects.

Life Cycle Assessment of a large commercial kelp farm in Shandong, China.

The environmental benefits of seaweed cultivation have gained a lot of attention, both in policy strategies and by private companies. Sustainability evaluations of seaweed farming have however focused on a very small part of global production of seaweed - on European cultivations at research and pilot-scales although Asia stands for 99 % of global production with China alone producing 60 %. In this study, we use Life Cycle Assessment (LCA) to evaluate the environmental performance of a 400-hectare Chinese kelp farm with a yearly harvest of 60,000 tons.

Rational Design Strategies for Nanozymes.

Nanozymes constitute an emerging class of nanomaterials with enzyme-like characteristics. Over the past 15 years, more than 1200 nanozymes have been developed, and they have demonstrated promising potentials in broad applications. With the diversification and complexity of its applications, traditional empirical and trial-and-error design strategies no longer meet the requirements for efficient nanozyme design.

Aal-circRNA-407 regulates ovarian development of Aedes albopictus, a major arbovirus vector, via the miR-9a-5p/Foxl axis.

Aedes albopictus shows a rapid global expansion and dramatic vectorial capacity for various arboviruses, thus posing a severe threat to global health. Although many noncoding RNAs have been confirmed to play functional roles in various biological processes in Ae. albopictus, the roles of circRNA remain a mystery.

[Bibliometric analysis on the literature characteristics of animal experiment research with acupuncture and moxibustion].

Objective: To compare the diversities in the literature characteristics of animal experiments with acupuncture and moxibustion (acu-moxibustion) published in both Chinese and English, so as to summarize the similarities and differences in the reporting content for the animal experiment research with acu-moxibustion in the journals at home and abroad.

Methods: The articles of animal experiments with acu-moxibustion published from 2016 to 2018 were searched from CNKI, Wanfang, SinoMed, PubMed and Web of Science databases. The articles were screened according to the inclusion and exclusion criteria, and the database was established by importing the essential information, e.

Electroacupuncture relieves hyperalgesia by regulating neuronal-glial interaction and glutamate transporters of spinal dorsal horns in rats with acute incisional neck pain.

Objective: Glial cells are involved in the analgesic effect of electroacupuncture (EA) in rats with chronic neurological pain. The objective of this study was to observe the role of neuronal-glial interaction and glutamate (Glu) transporters in EA-induced acute neck pain relief in rats.

Materials And Methods: Male rats were placed into the following five groups: control, model, EA Futu (LI18), EA Hegu (LI4)-Neiguan (PC6), and EA Zusanli (ST36)-Yanglingquan (GB34).

N-doped graphene aerogel cathode with internal aeration for enhanced degradation of p-nitrophenol by electro-Fenton process.

A columnar N-doped graphene aerogel (NGA) was successfully fabricated by one-step hydrothermal synthesis using L-hydroxyproline as reductant, N-doping, and swelling agent, and it was used as the cathode with internal aeration mode for the electro-Fenton degradation of p-nitrophenol. Owing to the stable solid-liquid-gas three-phase interface, more active defects, and modulated nitrogen dopants, the NGA cathode exhibited enhanced electrocatalytic activity. HO could be continuously electro-generated via a two-electron oxygen reduction, and the yield of HO was 153.

[κ-opioid receptor is involved in electroacupuncture analgesia via inhibition of spinal microglial Toll-like receptor 4 in neuropathic pain rats].

Objective: To observe the effect of electroacupuncture (EA) on the expression of lumbar spinal κ-opioid receptor (KOR) and Toll-like receptor 4(TLR4) in microglia in neuropathic pain rats, so as to explore the role of cross-talk between KOR and TLK4 in EA-induced alleviation of chronic neuropathic pain.

Methods: Wistar male rats were randomized into control, model, EA and EA plus KOR inhibitor (EA+inhibitor) groups (=18 in each group). The neuropathic pain model was established in rats by ligature of the right sciatic nerve.

Electroacupuncture Alleviates Hyperalgesia by Regulating CB1 Receptor of Spinal Cord in Incisional Neck Pain Rats.

Acupuncture therapy is effective in relieving postoperative pain of neck surgery, but its underlying mechanisms remain largely unknown. This study, in the incisional neck pain rat model, was designed to explore whether the endocannabinoid receptor 1 (CB1) in the cervical spinal cord is involved in the analgesic effect of electroacupuncture (EA) or not.The incisional neck pain model was established by making a longitudinal incision and applied EA treatment of Futu (LI18), Hegu-Neiguan (LI4-PC6), or Zusanli-Yanglingquan (ST36-GB34) for pain relief.

Mitigation of CyanoHABs Using Phoslock to Reduce Water Column Phosphorus and Nutrient Release from Sediment.

Cyanobacterial blooms can be stimulated by excessive phosphorus (P) input, especially when diazotrophs are the dominant species. A series of mesocosm experiments were conducted in a lake dominated by a cyanobacteria bloom to study the effects of Phoslock, a phosphorus adsorbent. The results showed that the addition of Phoslock lowered the soluble reactive phosphate (SRP) concentrations in water due to efficient adsorption and mitigated the blooms.

[Spinal cord Toll like receptor 4 and its co-stimulatory molecule heat shock protein 90 may parti-cipate in electroacupuncture analgesia in rats with chronic neuropathic pain].

Objective: To observe the effect of electroacupuncture (EA) on the expression of Toll like receptor 4(TLR4)and heat shock protein 90(HSP90) in the spinal cord of rats with chronic constriction injury (CCI) of sciatic nerve, so as to explore the mechanism of spinal cord TLR4 and HSP90 in alleviating chronic neuropathic pain by EA.

Methods: Male Wistar rats were randomized into control, model, EA, HSP90 inhibitor (inhibitor) and EA+ inhibitor groups (=10 in each group). The neuropathic pain model was established by ligature of the right sciatic nerve to induce CCI.

Exosomal miR-23a and miR-192, Potential Diagnostic Biomarkers for Type 2 Diabetes.

Background: Type 2 diabetes mellitus (T2D) is a common chronic disease, which lacks a specific diagnostic method. A substantial body of literature has demonstrated that the molecular constituents of extracellular vesicles (EVs) are promising as a novel biomarker for clinical diagnosis, especially exosomal miRNAs in biological fluids.

Methods: To find a diagnostic biomarker for T2D, we isolated exosomes from plasma and then quantitative PCR (qPCR) was used to detect the miRNA expression in plasma and exosomes from control subjects and T2D subjects.

GABAergic Inhibition of Spinal Cord Dorsal Horns Contributes to Analgesic Effect of Electroacupuncture in Incisional Neck Pain Rats.

Background: Acupuncture has shown to be effective in relieving post-surgical pain. Nonetheless, its underlying mechanisms remain largely unknown. In the present study, we investigated the effect of electroacupuncture (EA) on the expression of GABA, GABA-A receptor (R) and GABA-BR in the spinal cord dorsal horns (DHs), and the involved neural cells in rats with incisional neck pain.

Gender-Specific Association between Serum Uric Acid and Incident Fundus Arteriosclerosis in Chinese Population: A Retrospective Cross-Sectional Study.

Elevated levels of serum uric acid (SUA) were considered to be risk factors for cardiovascular disease, it has been found to be associated with increased arteriosclerosis. The aim of this study was to explore the gender specific relationship between SUA and fundus arteriosclerosis in a healthy population. In a retrospective cross-sectional study, 23474 individuals without diabetes and hypertension were included in the present study.

Fabrication of a double-layer membrane cathode based on modified carbon nanotubes for the sequential electro-Fenton oxidation of p-nitrophenol.

To improve the electrocatalytic efficiency of the cathode and provide a wider pH range in the electro-Fenton process, N-doped multi-walled carbon nanotubes (NCNTs) and ferrous ion complexed with carboxylated carbon nanotubes (CNT-COOFe) were used to fabricate the diffusion layer and catalyst layer of a membrane cathode, respectively. The morphology, structure, and composition of CNT-COOFe were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). The oxygen reduction performance of NCNT was evaluated using cyclic voltammetry (CV) and the rotating disk electrode technique (RDE).

Differentiation of Long Non-Coding RNA and mRNA Expression Profiles in Male and Female .

The Asia tiger mosquito () is an important vector of arboviruses, and females can transmit pathogens such as the dengue, zika, and chikungunya viruses. Understanding sex-related differences in this mosquito is fundamental for vector control. However, there are no reports of systematic characterization of long non-coding RNAs (lncRNAs) in male and female .

[Electroacupuncture alleviated incisional neck pain possibly by suppressing TNF-α expression and increasing IL-4/IL-4 receptor signaling in cervical dorsal part of spinal cord in incisio-nal neck pain rats].

Objective: To observe the effect of electroacupuncture (EA) on incisional pain and expression of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and interleukin-4 (IL-4) of cervical dorsal part of spinal cord in rats with incisional neck pain, so as to explore its analgesic mechanisms.

Methods: Eighty-four male SD rats were randomly divided into normal control, model, EA-Futu(LI18) and EA-Zusanli(ST36)-Yanglingquan(GB34, EA-ST36-GB34) groups (=21 in each group). The incisional neck pain model was established by making a longitudinal incision along the bilateral cervical thyroid regions and repeated mechanical separation stimulation.

A Novel Densovirus Isolated From the Asian Tiger Mosquito Displays Varied Pathogenicity Depending on Its Host Species.

Mosquito-borne viral diseases (MBVDs) continue to pose a significant global public health burden. Mosquito control remains a core intervention strategy in integrated mosquito management (IMM) programs to reduce the transmission of MBVDs. Mosquito densoviruses (MDVs) are mosquito-specific entomopathogenic viruses, and their attractive biological and pathogenic characteristics make MDVs potential biological control agents as alternatives to traditional chemical pesticides.

[High Mobility Group Box 1/ CD 24 Receptor/β-EP Signaling in "Zusanli" (ST 36) Region Contributes to Electroacupuncture Analgesia in Rats with Neuropathic Pain].

Objective: To observe the effect of electroacupuncture (EA) on the expression of high mobility group box 1 (HMGB 1) and its receptor CD 24 proteins and β-endorphin (β-EP) content in "Zusanli" (ST 36) region in rats with chronic constriction injury (CCI), so as to explore its mechanisms underlying pain relief.

Methods: Thirty male Wistar rats were rando-mized into control, CCI model and EA groups (＝ 10 rats in each). The neuropathic pain model was established by ligature of the left sciatic nerve to induce CCI in the model and EA groups, and sham operation was performed in rats of the control group.

Repeated electroacupuncture treatment attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats.

Background: Cumulated evidence reveals that glial cells in the spinal cord play an important role in the development of chronic neuropathic pain and are also complicated in the analgesic effect of EA intervention. But the roles of microgliacytes and astrocytes of spinal cord in the process of EA analgesia remain unknown.

Methods: A total of 120 male Wistar rats were used in the present study.

[Effect of Electroacupuncture Intervention on Expression of Synaptic Plasticity-related Molecules in Amygdala in Chronic Pain-negative Affection Rats].

Objective: To observe the effect of electroacupuncture (EA) on expression of synaptic plasticity-related glutamate N-methyl-D-aspartate (NMDA) receptor subunit NR 1, gamma-aminobutyric acid (GABA) receptor subunits (Aβ 2, B 1), etc. in the amygdala in chronic neuropathic pain negative affection (CNPPNA) rats, so as to reveal its mechanism underlying pain relief.

Methods: Male Wistar rats were randomized into normal control, CNPPNA model, EA, and anesthesia+EA (AEA)groups (=14 in each group, 8 for quantitative RT-PCR and 6 for immunofluorescence staining).

Preparation and Characterization of Poly-1,2,3-triazole with Chiral 2(5)-Furanone Moiety as Potential Optical Brightening Agents.

A series of novel heterocyclic polymers with fluorescent brightening properties are synthesized via Click polymerization. Fast synthesis of poly-1,2,3-triazoles ( ≥ 9.31 kDa) is described herein, with a high yield of up to 95%.