[Research of extracting and purifying taxol from the branches of Taxus media].

Objective: The study was to explore extracting and purifying technology of taxol from the branches of Taxus media.

Method: In extracting phase, the solvent extraction, ultrasonic extraction and the supercritical CO2 fluid extraction were studied respectively; In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane. The content of taxol was detected by HPLC.