[Study on correlation between connective tissue growth factor, macrophage colony-stimulating factor and cartilage degeneration in the osteoarthritis chondrocytes].

Objective: To investigate the expression of connective tissue growth factor (CTGF) in osteoarthritis chondrocytes, and the relationship between CTGF, macrophage colony-stimulating factor (M-CSF) and cartilage degeneration.

Methods: Ten New Zealand white rabbits were randomly divided into the normal group and OA model group established by Hulth's method. Sections were stained with Safranin O for histological examination.

[Experimental study of adoptive immunotherapy using CD3AK cells].

Aim: To analyze the immunological properties and biological activity of a monoclonal antibody (mAb) against CD3 molecule(yCD3), and to observe the tumor-suppressive activity of CD3AK cells in vitro and in vivo.

Methods: FCM was used to test the specificity of yCD3 and the immunological phenotype and cytokine production of CD3AK. 3H-TdR assay was used to measure the transformation of lymphocytes activated by yCD3.

[Identification of HCV antigen epitopes from a random 15-peptide library by anti-HCV polycloral antibody].

Aim: To analyze epitope recognized by anti-HCV antibodies; patients suffered from hepatitis C.

Methods: Anti-HCV Abs were purified from the patients serum through an affinity chromatography column which was prepared with sepharose 4B coupled with protein A. These Abs were used for biopanning of a phage-displayed random 15-peptide library.

[The Comparison between the Killing Effects of Two Anti-T Immunotoxins on Target Cells].

The key to killing target cells by immunotoxin depends on the specific recognition of antibody to target cell and the cytotoxic effect of toxin. The comparative study of the killing effects of two anti-T immunotoxins, CD5:Ricin and CD5:rRA, on target cells was performed. The elimination rate of immunotoxins was analysed by flow cytometry and MLR.