A Standardized Mouse Model for Wound Infection with .

is a highly drug-resistant pathogen known to impair wound healing and provoke inflammatory responses, potentially leading to immune dysregulation. This study aimed to systematically investigate the immune response mechanisms mediated by cytokines following infection through the development of a standardized wound model. Kunming mice were selected as experimental subjects and given 8 mm diameter lesions on their backs and inoculated with standard strains PAO1 and PA14.

Nitrogen-Doped Carbon Quantum Dots Activated Dandelion-Like Hierarchical WO for Highly Sensitive and Selective MEMS Sensors in Diabetes Detection.

High sensitivity, low concentration, and excellent selectivity are pronounced primary challenges for semiconductor gas sensors to monitor acetone from exhaled breath. In this study, nitrogen-doped carbon quantum dots (N-CQDs) with high reactivity were used to activate dandelion-like hierarchical tungsten oxide (WO) microspheres to construct an efficient and stable acetone gas sensor. Benefiting from the synergistic effect of both the abundant active sites provided by the unique dandelion-like hierarchical structure and the high reaction potential generated by the sensitization of the N-CQDs, the resulting 16 wt % N-CQDs/WO sensor shows an ultrahigh response value (/ = 74@1 ppm acetone), low detection limit (0.

Thanatin: A Promising Antimicrobial Peptide Targeting the Achilles' Heel of Multidrug-Resistant Bacteria.

Antimicrobial resistance poses an escalating threat to human health, necessitating the development of novel antimicrobial agents capable of addressing challenges posed by antibiotic-resistant bacteria. Thanatin, a 21-amino acid β-hairpin insect antimicrobial peptide featuring a single disulfide bond, exhibits broad-spectrum antibacterial activity, particularly effective against multidrug-resistant strains. The outer membrane biosynthesis system is recognized as a critical vulnerability in antibiotic-resistant bacteria, which thanatin targets to exert its antimicrobial effects.

Single-Atom Au-Functionalized Mesoporous SnO Nanospheres for Ultrasensitive Detection of Biomarker at Low Temperatures.

Semiconductor metal oxide gas sensors have been proven to be capable of detecting , one kind of foodborne bacteria, through monitoring the characteristic gaseous metabolic product 3-hydroxy-2-butanone. However, the detection still faces challenges because the sensors need to work at high temperatures and output limited gas sensing performance. The present study focuses on the design of single-atom Au-functionalized mesoporous SnO nanospheres for the sensitive detection of ppb-level 3-hydroxy-2-butanone at low temperatures (50 °C).

Mining and multifaceted applications of phage lysin for combatting Vibrio parahaemolyticus.

Vibrio parahaemolyticus, a prevalent foodborne pathogen found in both water and seafood, poses substantial risks to public health. The conventional countermeasure, antibiotics, has exacerbated the issue of antibiotic resistance, increasing the difficulty of controlling this bacterium. Phage lysins, as naturally occurring active proteins, offer a safe and reliable strategy to mitigate the impact of V.

Ultra-small gold nanoparticles embedded cyclodextrin metal-organic framework composite membrane to achieve antibacterial and humidity-responsive functions.

Cyclodextrin metal-organic framework (CD-MOF) is an edible and porous material that can serve as a template for synthesizing small-sized metal nanoparticles. However, its highly hydrophilic nature has limited its wider application. Herein, ultra-small gold nanoparticles (U-AuNPs) were loaded into CD-MOF to produce a composite material Au@CD-MOF.

Tryptophan metabolic pathway plays a key role in the stress-induced emotional eating.

Chronic stress disrupts the emotional and energetic balance, which may lead to abnormal behaviors such as binge eating. This overeating behavior alleviating the negative emotions is called emotional eating, which may exacerbate emotional instability and lead to obesity. It is a complex and multifaceted process that has not yet been fully understood.

Bimetallic Au and Pd Nanoparticles Modified WO Nanosheets for Enhancing the Sensitivity and Selectivity of Formaldehyde Assessment in Aquatic Products.

Formaldehyde, a common illegal additive in aquatic products, poses a threat to people's health and lives. In this study, a novel metal oxide semiconductor gas sensor based on AuPd-modified WO nanosheets (NSs) had been developed for the highly efficient detection of formaldehyde. WO NS modified with 2.

Parallel Monitoring of Glucose, Free Amino Acids, and Vitamin C in Fruits Using a High-Throughput Paper-Based Sensor Modified with Poly(carboxybetaine acrylamide).

Herein, a cost-effective and portable microfluidic paper-based sensor is proposed for the simultaneous and rapid detection of glucose, free amino acids, and vitamin C in fruit. The device was constructed by embedding a poly(carboxybetaine acrylamide) (pCBAA)-modified cellulose paper chip within a hydrophobic acrylic plate. We successfully showcased the capabilities of a filter paper-based microfluidic sensor for the detection of fruit nutrients using three distinct colorimetric analyses.

Alkaloid Precipitant Reaction Inspired Controllable Synthesis of Mesoporous Tungsten Oxide Spheres for Biomarker Sensing.

Highly porous sensitive materials with well-defined structures and morphologies are extremely desirable for developing high-performance chemiresistive gas sensors. Herein, inspired by the classical alkaloid precipitant reaction, a robust and reliable active mesoporous nitrogen polymer sphere-directed synthesis method was demonstrated for the controllable construction of heteroatom-doped mesoporous tungsten oxide spheres. In the typical synthesis, P-doped mesoporous WO monodisperse spheres with radially oriented channels (P-mWO-R) were obtained with a diameter of ∼180 nm, high specific surface area, and crystalline skeleton.

A procedure for producing an anti-AXL nanobody in E. coli.

As one of the receptors of the TAM family, AXL plays a vital role in stem cell maintenance, angiogenesis, immune escape of viruses and drug resistance against tumors. In this study, the truncated extracellular segment containing two immunoglobulin-like domains of human AXL (AXL-IG), which has been confirmed to bind growth arrest specific 6 (GAS6) by structural studies [1], was expressed in a prokaryotic expression system and then purified. Immunizing camelid with the purified AXL-IG as antigen could lead to the production of unique nanobodies composed of only variable domain of heavy chain of heavy-chain antibody (VHH), which are around 15 kD and stable.

Simultaneous and Sensitive Detection of Three Pesticides Using a Functional Poly(Sulfobetaine Methacrylate)-Coated Paper-Based Colorimetric Sensor.

Chlorpyrifos (CHL), profenofos (PRO) and cypermethrin (CYP) are widely used in combination to increase crop yields. However, these three pesticides can cause serious harm to human health and do not easily degrade. In this study, a novel visible paper sensor has been prepared successfully and different colorimetric reactions were utilized to detect the three pesticides simultaneously.

Hierarchical Flower-like WO Nanospheres Decorated with Bimetallic Au and Pd for Highly Sensitive and Selective Detection of 3-Hydroxy-2-butanone Biomarker.

, which is abundant in environment, can lead to many kinds of serious illnesses and even death. Nowadays, indirectly detecting the metabolite biomarker of , 3-hydroxy-2-butanone, has been verified to be an effective way to evaluate the contamination of . However, this detection approach is still limited by sensitivity, selectivity, and ppb-level detection limit.

Enhanced Response for Foodborne Pathogens Detection by Au Nanoparticles Decorated ZnO Nanosheets Gas Sensor.

is a hazardous foodborne pathogen that is able to cause acute meningitis, encephalitis, and sepsis to humans. The efficient detection of 3-hydroxy-2-butanone, which has been verified as a biomarker for the exhalation of , can feasibly evaluate whether the bacteria are contained in food. Herein, we developed an outstanding 3-hydroxy-2-butanone gas sensor based on the microelectromechanical systems using Au/ZnO NS as a sensing material.

2H-MoS/CoO nanohybrid with type I nitroreductase-mimicking activity for the electrochemical assays of nitroaromatic compounds.

A type I nitroreductase-mimicking nanocatalyst based on 2H-MoS/CoO nanohybrids for trace nitroaromatic compounds detection is reported in this work. For the preparation of nanocatalyst, ultrathin CoO nanoflakes array was in-situ grown onto 2H-MoS nanosheets forming three-dimensional (3D) nanohybrid with large specific surface area as well as abundant active sites. The as-prepared nanocatalyst shows a specific affinity as well as high catalytic activity towards nitroaromatic compounds.

Ultra-efficient trimethylamine gas sensor based on Au nanoparticles sensitized WO nanosheets for rapid assessment of seafood freshness.

Trimethylamine (TMA) is an organic amine gas used as an important index to assess the seafood freshness. In this work, an ultra-efficient trimethylamine gas sensor was constructed by ultrasonic treatment loading of Au nanoparticles (∼4 nm) on WO nanosheets prepared by solvothermal self-assembly method. The response of the sensor to 25 ppm TMA was as high as 217.

Structural and functional properties of soluble Antarctic krill proteins covalently modified by rutin.

New polyphenol-protein conjugates were successfully prepared by covalently crosslinking soluble Antarctic krill proteins with rutin (SAKPs-rutin). The physico-chemical and functional properties of SAKPs-rutin conjugates were systematically evaluated by measuring the changes in interfacial tension, structural conformation, and emulsifying ability, etc. The results showed that SAKPs-rutin conjugates possessed higher surface hydrophobicity, surface charge, and thermal denaturation temperature, and lower β-sheet conformation compared to native SAKPs.

Fenton reaction-assisted photodynamic inactivation of calcined melamine sponge against Salmonella and its application.

Photodynamic inactivation (PDI) is an effective alternative to traditional antibiotics to broadly kill bacteria. This study aimed to develop a potent PDI system by coupling calcinated melamine sponges (CMSs) with the Fenton reaction. The results showed that CMS calcined at 350 ℃ was successfully carbonized with intact and porous structures, and it possessed excellent hydrophilicity and photothermal conversion performance.

Bimetallic Au@Pt Nanocrystal Sensitization Mesoporous α-FeO Hollow Nanocubes for Highly Sensitive and Rapid Detection of Fish Freshness at Low Temperature.

In this work, we present a new metal oxide semiconductor gas sensor for detecting trimethylamine (TMA) by bimetal Au@Pt-modified α-FeO hollow nanocubes (NCs) as sensing materials. The structure and morphological characteristics of Au@Pt/α-FeO were evaluated through multiple analyses, and their gas-sensitive performance was investigated. Compared with the pristine α-FeO NC sensor, the sensor based on Au@Pt/α-FeO NCs exhibited faster response time (5 s) and higher response (/ = 32) toward 100 ppm TMA gas at a lower temperature (150 °C).

Comparison on the Growth Heterogeneity of Vibrio parahaemolyticus Coupled with Strain Source and Genotype Analyses in Different Oligotrophic Conditions.

Abstract: Vibrio parahaemolyticus is an important foodborne pathogen in aquatic products that can survive long term in an oligotrophic environment and maintain pathogenicity. In this study, the growth curves of 38 strains of V. parahaemolyticus (pathogenic and environmental strains) under different oligotrophic conditions (tryptone soy broth [TSB] and TSB medium diluted 2, 4, and 6 times) were simulated and their growth heterogeneities were compared.

Enzyme-induced Cu/Cu conversion as the electrochemical signal for sensitive detection of ethyl carbamate.

Traditional enzyme-linked immunosorbent assay (t-ELISA) method suffers from its relatively low sensitivity or accuracy in the detection of trace level of analyte in complicated samples. In this work, to extend the application of ELISA in practical samples, a newly electrochemical immunoassay (ECIA) was developed based on an enzyme-induced Cu/Cu conversion for the determination of ethyl carbamate (EC). Wherein, three rounds of signal transformation-the catalysis of ALP enzyme, the conversion of Cu/Cu and signal output of square wave voltammetry (SWV), can be realized to obtain higher sensitivity as compared to t-ELISA.

Effects of ultrasound-assisted basic electrolyzed water (BEW) extraction on structural and functional properties of Antarctic krill (Euphausia superba) proteins.

A novel protein extraction method of ultrasound-assisted basic electrolyzed water (BEW) was proposed, and its effects on the structural and functional properties of Antarctic krill proteins were investigated. Results showed that BEW reduced 30.9% (w/w) NaOH consumption for the extraction of krill proteins, and its negative redox potential (-800 ~ -900 mV) protected the active groups (carbonyl, free sulfhydryl, etc.

Strawberry-like SiO/Ag nanocomposites immersed filter paper as SERS substrate for acrylamide detection.

In this work, based on the strawberry-like SiO/Ag nanocomposites (SANC) immersed filter paper, a newly surface-enhanced Raman scattering (SERS) substrate was constructed for the detection of acrylamide (AAm) in food products. To construct filter paper-based SANC (F-SANC) SERS substrates, SiO nanoparticles (SNP) were firstly synthesized and acted as carriers. After that, the in-situ preparation of silver nanoparticles (Ag NP) on SNP surface was carried out to form the strawberry-like three-dimensional (3D) structure of SANC.

Bimetallic PtCu Nanocrystal Sensitization WO Hollow Spheres for Highly Efficient 3-Hydroxy-2-butanone Biomarker Detection.

As a foodborne bacterium, () can cause serious diseases and even death to weak people. 3-Hydroxy-2-butanone (3H-2B) has been proven to be a biomarker for exhalation of LM. Detection of 3H-2B is a fast and effective method for determining whether the food is infected.

Microevolution of Vibrio parahaemolyticus Isolated from Clinical, Acute Hepatopancreatic Necrosis Disease Infecting Shrimps, and Aquatic Production in China.

Vibrio parahaemolyticus is the leading cause of bacteria-associated foodborne diarrheal diseases and specifically causes early mortality syndrome (EMS), which is technically known as acute hepatopancreatic necrosis disease (AHPND), a serious threat to shrimp aquaculture. To investigate the genetic and evolutionary relationships of V. parahaemolyticus in China, 184 isolates from clinical samples (VPC, n=40), AHPND-infected shrimp (VPE, n=10), and various aquatic production sources (VPF, n=134) were collected and evaluated by a multilocus sequence analysis (MLST).