Exploring the molecular mechanisms by which secretory phospholipase a2 regulates lymphatic endothelial cell dysfunction by activating macrophages.

This study offers new insights into the dual role of secretory phospholipase A2 (sPLA2) in lymphedema, highlighting its impact on lymphatic endothelial cell (LEC) functionality. Through transcriptomic analyses and co-culture experiments, we observed that sPLA2 has both protective and detrimental effects on human LECs (HLECs), mediated by macrophage activation. Our findings reveal that while low levels of sPLA2 promote LEC health, excessive sPLA2 leads to dysfunction, emphasizing the significance of the sPLA2/PLA2R axis and arachidonic acid metabolism (AA) in lymphedema pathology.

Using Co-Culture to Functionalize Clostridium Fermentation.

Clostridium fermentations have been developed for producing butanol and other value-added chemicals, but their development is constrained by some limitations, such as relatively high substrate cost and the need to maintain an anaerobic condition. Recently, co-culture is emerging as a popular way to address these limitations by introducing a partner strain with Clostridium. Generally speaking, the co-culture strategy enables the use of a cheaper substrate, maintains the growth of Clostridium without any anaerobic treatment, improves product yields, and/or widens the product spectrum.

Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture of G117 and recombinant 1A1.

An engineered 1A1 strain (BsADH2) expressing a secondary alcohol dehydrogenase (CpSADH) was co-cultured with G117 under an aerobic condition. During the fermentation on glucose, BsADH2 depleted oxygen in culture media completely and created an anaerobic environment for G117, an obligate anaerobe, to grow. Meanwhile, lactate produced by BsADH2 was re-assimilated by G117.

Production of isopropyl and butyl esters by Clostridium mono-culture and co-culture.

Production of esters from the acetone-butanol-ethanol (ABE) fermentation by Clostridium often focuses on butyl butyrate, leaving acetone as an undesired product. Addition of butyrate is also often needed because ABE fermentation does not produce enough butyrate. Here we addressed the problems using Clostridium beijerinckii BGS1 that preferred to produce isopropanol instead of acetone, and co-culturing it with Clostridium tyrobutyricum ATCC 25,755 that produced butyrate.

Publisher Correction: Intensity-dependent modulation of optically active signals in a chiral metamaterial.

This corrects the article DOI: 10.1038/ncomms14602.

Intensity-dependent modulation of optically active signals in a chiral metamaterial.

Chiral media exhibit optical phenomena that provide distinctive responses from opposite circular polarizations. The disparity between these responses can be optimized by structurally engineering absorptive materials into chiral nanopatterns to form metamaterials that provide gigantic chiroptical resonances. To fully leverage the innate duality of chiral metamaterials for future optical technologies, it is essential to make such chiroptical responses tunable via external means.

Electrically Tunable Harmonic Generation of Light from Plasmonic Structures in Electrolytes.

An emerging trend in plasmonics is to exploit nanostructured metals as a self-contained electrooptic platform with simultaneously supported electrical and optical functions. When it comes to nonlinear optics, this dual electrical and optical functionality offers an exciting potential to enable electrically controlled wave mixing processes in various nanometallic systems. Here we demonstrate tunable nonlinear generation of light enabled by an electrically active plasmonic crystal in aqueous electrolytic solutions.

An Active Metamaterial Platform for Chiral Responsive Optoelectronics.

Chiral-selective non-linear optics and optoelectronic signal generation are demonstrated in an electrically active photonic metamaterial. The metamaterial reveals significant chiroptical responses in both harmonic generation and the photon drag effect, correlated to the resonance behavior in the linear regime. The multifunctional chiral metamaterial with dual electrical and optical functionality enables transduction of chiroptical responses to electrical signals for integrated photonics.

Backward phase-matching for nonlinear optical generation in negative-index materials.

Metamaterials have enabled the realization of unconventional electromagnetic properties not found in nature, which provokes us to rethink the established rules of optics in both the linear and nonlinear regimes. One of the most intriguing phenomena in nonlinear metamaterials is 'backward phase-matching', which describes counter-propagating fundamental and harmonic waves in a negative-index medium. Predicted nearly a decade ago, this process is still awaiting a definitive experimental confirmation at optical frequencies.

Metamaterials enable chiral-selective enhancement of two-photon luminescence from quantum emitters.

The amplification of chirally modified, non-linear signals from quantum emitters is demonstrated by manipulating the geometric chirality of resonant plasmonic nanostructures. The chiral center of the metamaterial is opened and emitters occupy this light-confining and chirally sensitive region. Non-linear emission signals are enhanced by 40× that of the emitters not embedded in the metamaterial and display a 3× contrast for the opposite circular polarization.

Electrifying photonic metamaterials for tunable nonlinear optics.

Metamaterials have not only enabled unprecedented flexibility in producing unconventional optical properties that are not found in nature, they have also provided exciting potential to create customized nonlinear media with high-order properties correlated to linear behaviour. Two particularly compelling directions are active metamaterials, whose optical properties can be purposely tailored by external stimuli in a reversible manner, and nonlinear metamaterials, which enable intensity-dependent frequency conversion of light waves. Here, by exploring the interaction of these two directions, we leverage the electrical and optical functions simultaneously supported in nanostructured metals and demonstrate electrically controlled nonlinear optical processes from a metamaterial.

Nonlinear imaging and spectroscopy of chiral metamaterials.

A chiral metamaterial produces both distinguishable linear and non-linear resonant features when probed with left and right circularly polarized light. The material demonstrates a linear transmission contrast of 0.5 between left and right circular polarizations and a 20× contrast between second-harmonic responses from the two incident polarizations.

Giant chiral optical response from a twisted-arc metamaterial.

We demonstrate enormously strong chiral effects from a photonic metamaterial consisting of an array of dual-layer twisted-arcs with a total thickness of ∼ λ/6. Experimental results reveal a circular dichroism of ∼ 0.35 in the absolute value and a maximum polarization rotation of ∼ 305°/λ in a near-infrared wavelength region.

Scattering reduction at near-infrared frequencies using plasmonic nanostructures.

Novel fabrication, detection and analysis approaches were employed to experimentally demonstrate scattering reduction by a plasmonic nanostructure operating at 1550 nm. The nanostructure consisted of a silicon nanorod surrounded by a plasmonic metamaterial cover comprised of eight gold nanowires and was fabricated by a combination of electron beam lithography, focused ion beam milling and dry and wet etching. The optical standing wave pattern of the device in the near-field was obtained using heterodyne near-field scanning optical microscopy.

Nanorod orientation dependence of tunable Fano resonance in plasmonic nanorod heptamers.

We present orientation dependence and tuning of Fano resonances in plasmonic gold nanorod heptamer nanostructures. The heptamer structures were formed by surrounding a central circular nanoparticle by six satellite nanorods in a hexagonal arrangement and the heptamer structures were then embedded in a thin flexible membrane. The Fano resonance was found to depend on the orientation of the nanorods within the heptamer and for a given orientation, the Fano resonance was dynamically tuned and its symmetry lowered by application of uniaxial mechanical stress.

Dynamic tuning and symmetry lowering of Fano resonance in plasmonic nanostructure.

We present dynamic tuning and symmetry lowering of Fano resonances in gold heptamers accomplished by applying uniaxial mechanical stress. The flexible heptamer structure was obtained by embedding the seven-gold-nanocylinder complex in a polydimethylsiloxane membrane. Under uniaxial stress, the Fano resonance exhibited opposite spectral shifts for the two orthogonal polarizations parallel and perpendicular to the mechanical stress.

Thermo-optically tunable silicon photonic crystal light modulator.

We designed, fabricated, and characterized a thermo-optically tunable compact (10 μm × 10 μm) silicon photonic crystal (PhC) light modulator that operates at around 1.55 µm for TE polarization. The operational principle of the device is the modulation of the cutoff frequency in a silicon-based line defect PhC.

Cell encapsulation and oxygenation in nanoporous microcontainers.

With strides in stem cell biology, cell engineering and molecular therapy, the transplantation of cells to produce therapeutic molecules endogenously is an attractive and achievable alternative to the use of exogenous drugs. The encapsulation of such cell transplants in semi-permeable, nanoporous constructs is often required to protect them from immune attack and to prevent their proliferation in the host. However, effective graft immunoisolation has been mostly elusive owing to the absence of a high-throughput method to create precisely controlled, high-aspect-ratio nanopores.