Publications by authors named "Yongguo Huang"
Article Synopsis
- Hepatitis B virus (HBV) remains a significant public health issue, particularly in China, prompting a study to assess its epidemiological and clinical characteristics in children to help control its spread in Wuhan.
- A retrospective analysis of over 115,000 children aged 0-18 revealed overall low positive detection rates for various HBV serological markers, with notable differences in results between males and females.
- The study identified 22 distinct HBV serological expression patterns, with the majority being more common among neonatal patients, while the overall detection rate showed a decline over the analyzed four years.
Objective: Accumulating Studies implies that long-chain non-coding RNA (lncRNA) plays a vital regulatory role in the occurrence and progression of tumors. This study aimed to explore the function and mechanism of lncRNA HLA-F antisense RNA 1 (HLA-F-AS1) in colorectal cancer (CRC).
Methods: Expressions of HLA-F-AS1, miR-330-3p and profilin 1 (PFN1) mRNA in CRC tissues were detected by RT-PCR.
MicroRNA-520d-3p (miR-520d-3p) is a novel cancer-related miRNA and functions as a tumor suppressor in human cancers. However, the expression patterns and mechanisms of miR-520d-3p involved in hepatocellular carcinoma (HCC) remain rarely known. Here, we found that the expression levels of miR-520d-3p in HCC tissues and cells were significantly lower than in tumor-adjacent tissues and L02 cells.
View Article and Find Full Text PDFUsing a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.
View Article and Find Full Text PDF[Preparation of anti HBs monoclonal antibody and its combination characterization to both wild-type HBsAg and immune escape mutant HBsAg].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
June 2008
Aim: To prepare monoclonal antibodies against HBsAg and estimate its binding capacity to both wild-type and varies of immune escape mutant-type HBsAg.
Methods: Using self-made the anti-HBs G6 mAb and HRP-labeled goat anti-HBs antibody, A sandwich ELISA for detection both wild-type and varies of immune escape mutant-type HBsAg has been developed. Applying this assay, to detect 17 species of whole gene wild-type and recombination expressed HBsAg which varied in "a" determinant.