Biosynthesis of poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol.

As the most representative biodegradable thermoplastic, poly(3-hydroxybutyrate) (P3HB) has a limited range of applications because of its poor thermal and physical properties. To improve its properties, a novel biosynthetic system was designed to produce poly(3-hydroxypropionate-co-3-hydroxybutyrate) (P(3HP-co-3HB)) with fully controllable structures from inexpensive carbon source. In this system, two parallel synthetic pathways controlled by independent regulatory systems were used to produce the 3HP and 3HB monomers, respectively.

Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coli.

Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, a P3HP biosynthetic pathway from glycerol was constructed in recombinant Escherichia coli. The genes for glycerol dehydratase and its reactivating factor (dhaB123 and gdrAB, from Klebsiella pneumoniae), propionaldehyde dehydrogenase (pduP, from Salmonella typhimurium), and polyhydroxyalkanoate synthase (phaC1, from Cupriavidus necator) were cloned and expressed in E.

Prokaryotic expression and characterization of human AC3-33 protein.

The transcription factor, AP-1, plays an important role in cellular proliferation, transformation and death. We previously showed that AC3-33 (GenBank name: c3orf33, FLJ31139), significantly inhibited transcriptional activity of AP-1. In this study, we report a method to express and purify AC3-33 in E.