Research note: Establishment of a multiplex PCR detection system for different infectious bronchitis virus strain genotypes in laying hens.

This study produced a method for the fast detection and identification of five strains of infectious bronchitis virus (IBV), including the currently prevalent wild-type QX and G Ⅵ-1 strains, in addition to the Mass, 4/91, and LDT3 strains that are used as vaccines. Based on the spike 1 (S1) glycoprotein gene sequences of the IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains, five sets of customized primers, showing excellent specificity, were designed and synthesized to target the appropriate conserved sequences. After repeated improvement, a multiple PCR detection approach with strong specificity for the simultaneous detection of IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains was initially established.

Research on Efficient Electromagnetic Shielding Performance and Modulation Mechanism of Aero/Organo/Hydrogels with Gravity-Induced Asymmetric Gradient Structure.

To eliminate electromagnetic pollution, it is a challenging task to develop highly efficient electromagnetic shielding materials that integrate microwave absorption (MA) performance with high shielding capability and achieve tunability in shielding performance. Asymmetrically structured aero/organo/hydrogels with a progressively changing concentration gradient of liquid metal nanoparticles (LMNPs), induced by gravity, are prepared by integrating the conductive fillers TiCT MXene and LMNPs into a dual-network structure composed of polyvinyl alcohol and cellulose nanofibers. Benefiting from the unique structure, which facilitates the absorption-reflection-reabsorption process of electromagnetic waves along with conductive fillers and the porous structure, three types of gels demonstrate efficient shielding performance.

Research on magnetic nonchain transport of steel cartridge casing ammunition.

Magnetic force is used to drive ferromagnetic objects by shortening the magnetic flux path to reduce magnetic reluctance and increase magnetic conductivity. Based on this characteristic, magnetic force drives have been widely applied in various fields, such as military, transportation, and engineering, attracting significant attention. This paper proposes a method for the nonchain transport of steel shell ammunition based on magnetic force.

Generation of an infectious cDNA clone for NADC30-like PRRSV.

The porcine reproductive and respiratory syndrome virus (PRRSV) is a highly significant infectious disease that poses a substantial threat to the global pig industry. In recent years, the NADC30-like strain has gradually emerged as prevalent in China, causing a profound impact on the country's pig farming industry. Therefore, it is important to conduct an in-depth study on the characteristics and gene functions of the NADC30-like strain.

Polymorphism and expression of in relation to reproductive performance in Jining Grey goats.

Understanding the molecular mechanism of mammalian reproduction (puberty and prolificacy) will play a part in improving animal reproductive performance. (glutamate dehydrogenase 1) is important for mammalian reproduction, as shown in previous studies; however, its roles in puberty and prolificacy have rarely been reported. In this study, we designed seven pairs of primers (P1 to P7) for cloning and sequencing genomic DNA of Jining Grey goats and Liaoning Cashmere goats.

Microstructure optimization strategy of ZnInS/rGO composites toward enhanced and tunable electromagnetic wave absorption properties.

Although microstructure optimization is an effective strategy to improve and regulate electromagnetic wave (EMW) absorption properties, preparing microwave absorbents with enhanced EMW absorbing performance and tuned absorption band by a simple method remains challenging. Herein, ZnInS/reduced graphene oxide (rGO) composites with flower-like and cloud-like morphologies were fabricated by a convenient hydrothermal method. The ZnInS/rGO composites with different morphologies realize efficient EMW absorption and tunable absorption bands, covering a wide frequency range.

A Broad-Spectrum Phage Endolysin (LysCP28) Able to Remove Biofilms and Inactivate Strains.

is a gram-positive, anaerobic, spore-forming bacterium capable of producing four major toxins which cause disease symptoms and pathogenesis in humans and animals. strains carrying enterotoxins can cause food poisoning in humans and are associated with meat consumption. An endolysin, named LysCP28, is encoded by orf28 from bacteriophage BG3P.

Hypertension Subtypes, Mortality Risk, and Differential Effects Between Two Hypertension Guidelines.

Aim: To examine which hypertension subtypes are primarily responsible for the difference in the hypertension prevalence and treatment recommendations, and to assess their mortality risk if 2017 American College of Cardiology (ACC)/American Heart Association (AHA) hypertension guideline were adopted among Chinese adults.

Methods: We used the nationally representative data of China Health and Retirement Longitudinal Study (CHARLS) to estimate the differences in the prevalence of isolated systolic hypertension (ISH), systolic diastolic hypertension (SDH) and isolated diastolic hypertension (IDH) between the 2017 ACC/AHA and the 2018 China Hypertension League (CHL) guidelines. We further assessed their mortality risk using follow-up data from the China Health and Nutrition Survey (CHNS) by the Cox model.

piR-823 inhibits cell apoptosis via modulating mitophagy by binding to PINK1 in colorectal cancer.

Mitophagy plays a vital role in the maintenance of mitochondrial homeostasis and tumorigenesis. Noncoding RNA piR-823 contributes to colorectal tumorigenesis. In this study, we aim to evaluate piR-823-mediated mitophagy and its mechanistic association with colorectal cancer (CRC).

Expression, Regulation, and Role of an Oligopeptide Transporter: PEPT1 in Tumors.

PEPT1 is a vital member of the proton-dependent oligopeptide transporters family (POTs). Many studies have confirmed that PEPT1 plays a critical role in the absorption of dipeptides, tripeptides, and pseudopeptides in the intestinal tract. In recent years, several studies have found that PEPT1 is highly expressed in malignant tumor tissues and cells.

Time to Clinical Benefit of Intensive Blood Pressure Lowering in Patients 60 Years and Older With Hypertension: A Secondary Analysis of Randomized Clinical Trials.

Importance: Recent guidelines recommend a systolic blood pressure (BP) goal of less than 150 mm Hg or even 130 mm Hg for adults aged 60 years or older. However, harms from intensive BP treatments occur immediately (eg, syncope, fall), and benefits for cardiovascular event reduction emerge over time. Therefore, harms with low chance of benefit need to be clearer, particularly for those with limited life expectancy.

The Broad Host Range Phage vB_CpeS_BG3P Is Able to Inhibit Growth.

Clostridium perfringens is an important pathogen for both humans and animals, causing human foodborne disease and necrotic enteritis in poultry. In the present study, a C. perfringens-specific phage, vB_CpeS_BG3P (designated as BG3P hereafter), was isolated from chicken farm sewage.

Protection of Ducklings from Duck Hepatitis A Virus Infection with ELPylated Duck Interferon-α.

Duck viral hepatitis type I (DVH I) is a lethal disease in ducklings caused by duck hepatitis A virus (DHAV). Although the commercial vaccine is available for vaccination of one-day-old ducklings or breeder ducks, the disease is still prevalent due to the delayed immune response in ducklings and variable maternal antibody levels in breeder duck flocks. To explore the feasibility of duck interferon-α (DuIFN-α) for control of DVH I, DuIFN-α was expressed as an elastin-like polypeptide (ELP) fusion protein (ELP-DuIFN-α) in E.

Prevalence and Numbers of Diabetes Patients with Elevated BMI in China: Evidence from a Nationally Representative Cross-Sectional Study.

China is facing the challenges of the increasing burden of diabetes and obesity; the prevalence and numbers of diabetes patients with obesity or overweight are still unclear. Nationally representative data from the China Health and Retirement Longitudinal Study (CHARLS) were used to estimate the prevalence of diabetes patients with elevated BMI, the recommendation rate for antidiabetic medication, the blood glucose control rate, and the corresponding population size. The prevalence of diabetes patients with elevated BMI was 9.

Lightweight TiO@C/Carbon Fiber Aerogels Prepared from TiCT/Cotton for High-Efficiency Microwave Absorption.

Carbon fiber aerogel (CFA) derived from cotton wool as a potential microwave absorbing material has received intensive attention owing to the low density, high conductivity, large surface area, and low cost, but its applications are limited by the relatively high complex permittivity. To solve this problem, TiO@C (derived from TiCT) is introduced into CFA to prepare lightweight TiO@C/CFA composites based on electromagnetic (EM) parameter optimization and enhanced EM wave attenuation performance. The microwave absorption capacity of TiO@C/CFA-2 composite is obviously better than that of CFA.

REC8 enhances stemness and promotes metastasis of colorectal cancer through BTK/Akt/β-catenin signaling pathway.

Cancer/testis antigens (CTAs) are often aberrantly expressed in cancer stem cells (CSCs) which are responsible for tumor metastasis. Rec8 meiotic recombination protein (REC8), a member of CTAs, shares distinct roles in various cancers, while its contribution to CSCs and colorectal cancer (CRC) remains unclear. We found that overexpression of REC8 facilitated the migration and invasion of CRC cells (DLD-1 and SW480 cells) in vitro and promoted the liver metastasis of CRC in vivo.

Soluble TREM-1, as a new ligand for the membrane receptor Robo2, promotes hepatic stellate cells activation and liver fibrosis.

Triggering receptor expressed on myeloid cells-1 (TREM-1) exists in two forms: a transmembrane form and a soluble form (sTREM-1). The levels of sTREM-1 are elevated in supernatants of activated HSCs. However, the role of sTREM-1 in HSC activation and liver fibrosis remains undefined.

Y-box binding protein 1 augments sorafenib resistance the PI3K/Akt signaling pathway in hepatocellular carcinoma.

Background: Sorafenib is the first-line treatment for patients with advanced hepatocellular carcinoma (HCC). Y-box binding protein 1 (YB-1) is closely correlated with tumors and drug resistance. However, the relationship between YB-1 and sorafenib resistance and the underlying mechanism in HCC remain unknown.

LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure.

Background: Triggering receptor expressed on myeloid cells-1 (TREM-1) controls the mobilization of inflammatory cells in response to injury and consequently enhances liver damage. LR12 is a TREM-1 inhibitory peptide. However, the role of LR12 in acute liver failure (ALF) has remained elusive.

Adsorption behavior of heavy metal ions on a polymer-immobilized amphoteric biosorbent: Surface interaction assessment.

Here we unveiled a novel magnetically separable amphoteric biosorbent (PD-FeO@CCS) and investigated its adsorption behavior toward two classes of heavy metals, hexavalent chromium (Cr(VI)) and copper (Cu(II)) ions from water. Results indicated that the adsorption behavior of PD-FeO@CCS for Cr(VI) was well described by Langmuir model; while for Cu(II) adsorption, the Freundlich model was the better one. Based on the kinetic results, both Cr(VI) and Cu(II) adsorption on PD-FeO@CCS fitted well with the pseudo-second-order kinetic model.

Structural design of magnetic biosorbents for the removal of ciprofloxacin from water.

Magnetic biosorbents with specific morphological and molecular structure (PMCCs) were designed for the removal of ciprofloxacin (CIP) from water. Radical polymerization method was applied to immobilize the designed polymer brushes onto core-shell shaped magnetic microspheres to fabricate PMCCs. PMCCs exhibited a maximum adsorption capacity of 527.

Self-Assembled 3D Flower-like Composites of Heterobimetallic Phosphides and Carbon for Temperature-Tailored Electromagnetic Wave Absorption.

Bimetallic cobalt-nickel phosphides as a microwave absorber with a well-defined 3D hierarchical flower-like architecture featuring the ultrathin 2D subunits are very unusual and rarely reported. Herein, for the first time, we successfully prepared 3D flower-like CoNi-P/C composites with 2D nanosheet subunits via a one-pot solvothermal self-assembled strategy followed by a one-step carbonization-phosphorization process. Interestingly, the chemical composition and electromagnetic (EM) wave absorption performance of composites are highly influenced by the calcination temperature.

Simultaneous adsorption and reduction of hexavalent chromium on the poly(4-vinyl pyridine) decorated magnetic chitosan biopolymer in aqueous solution.

Poly(4-vinyl pyridine) decorated magnetic chitosan biopolymer (VMCP), as an absorbent and reductant, was prepared and used to remove hexavalent chromium (Cr(VI)) from aqueous solution. Compared with undecorated magnetic biopolymer, VMCP exhibited significantly improved removal performance under identical experimental conditions. The kinetics, isotherms, and thermodynamics of Cr(VI) adsorption onto VMCP were investigated.

Splenectomy Promotes Macrophage Polarization in a Mouse Model of Concanavalin A- (ConA-) Induced Liver Fibrosis.

Background: Splenectomy can improve liver function and survival in patients with autoimmune hepatitis (AIH) and liver cirrhosis. We investigated the underlying mechanism in a mouse model of concanavalin A- (ConA-) induced liver fibrosis.

Methods: We used ConA to induce immune liver fibrosis in BALB/c mice.