[Expression and significance of hTERT, c-myc and Ki-67 in hepatocellular carcinoma].

Aim: To study the correlation of expression of hTERT, c-myc and Ki-67 with the clinical pathological feature of hepatocellular carcinoma and explore the relationship among the three factors.

Methods: The expressions of hTERT, c-myc and Ki-67 in 37 cancer tissues and 5 normal tissues were assessed by immunohistochemical method.

Results: The positive rates of hTERT, c-myc and Ki-67 in the cancer tissue were higher than those in normal tissues (P<0.

[Expression of GFP gene in mouse early embryo produced by sperm-mediated gene transfer].

The efficiency of the exogenous DNA transfecting mouse sperm was studied by the DIG end labeled and immunohistochemistry technology. The results suggested that: the efficiency of transfecting positive rate of individual mouse sperm was distinct difference (P < 0.01), and the average rate was 13%.

[Nuclear transfer of goat somatic cells transgenic for human lactoferrin].

Transgenic animal mammary gland bioreactors are being used to produce recombinant proteins with appropriate post-translational modifications, and nuclear transfer of transgenic somatic cells is a more powerful method to produce mammary gland bioreactor. Here we describe efficient gene transfer and nuclear transfer in goat somatic cells. Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene, and the endogenous start condon was replaced by that of human LF gene.