Publications by authors named "Yong-tao QU"
[Impacts of hypoxia on the features and chemoresistance of cancer stem cells in Hep-2 cells and underlying mechanism].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
March 2012
Objective: To investigate the effects of hypoxia on the features and chemoresistance of cancer stem cells in Hep-2 cells and underlying mechanism.
Methods: The shRNA interference recombinant plasmid targeting HIF-1α was synthesized and transfected into Hep-2 cells. The HIF-1α knockdown Hep-2 cells were established after clonal selection and the expression of HIF-1α was measured.
[Expression of hypoxia inducible factor-1α and correlated target genes in human laryngeal carcinoma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
April 2012
Objective: To detect the expression of hypoxia inducible factor 1 alpha (HIF-1α), glucose transporter protein-1 (GLUT-1) and vascular endothelial growth factor (VEGF) in human laryngeal carcinoma tissue, and to study the relationship between hypoxia and HIF-1α, GLUT-1, VEGF in human laryngeal carcinoma Hep-2 cells and to explore the effect of HIF-1α, GLUT-1 and VEGF as endogenous hypoxic markers on laryngeal carcinoma.
Methods: The expression levels of HIF-1α, GLUT-1 and VEGF were detected in 35 cases of laryngeal carcinoma by SP immunohistochemical methods and in Hep-2 cells by SP immunocytochemical methods. The relationship between HIF-1α and GLUT-1, VEGF protein expression was analyzed.
[Cancer stem cells play an important role in resistance of laryngeal squamous cancer to irradiation mediated by hypoxia].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
December 2011
Article Synopsis
- The study aimed to investigate whether laryngeal cancer stem cells exhibit resistance to irradiation under hypoxic conditions and to identify the underlying mechanisms involved.
- CD133(+) cells were isolated from Hep-2 cells and cultured in both hypoxia and normal oxygen levels, with different dosages of X-ray exposure to assess growth inhibition and colony formation.
- Results indicated that CD133(+) cells in hypoxia had a lower growth inhibition ratio and higher colony formation compared to normoxic cells, suggesting that hypoxia contributes to their radioresistance through increased expression of specific proteins like DNA-PKcs and Survivin.