CircFBXW7 alleviates glioma progression through regulating miR-23a-3p/PTEN axis.

Increasing evidence has confirmed that circular RNAs (circRNAs) are involved in regulating the development and progression of various tumors. The aim of this study was to examine the effect of circFBXW7 on the progression of glioma and to determine its underlying mechanism. qRT-PCR was performed to measure the expression of circFBXW7, miR-23a-3p, and PTEN in tissues and cell lines of glioma.

[Clinical Characteristics of 223 Chinese Patients with Hemophilia in A Medical Center of Gansu Province in China].

Objective: To analyze the clinical characteristics, diagonsis and treatment of patients with hemophilia in Gansu province of China.

Methods: The clinical data of 223 cases of hemophilia in our center between January 2010 and May 2015 were collected and analyzed retrospectively, these 223 cases of hemophilia were from 14 cities in Gansu and neighboring provinces, including 203 cases of hemophili A (HA) and 20 cases of hemophili B (HB), among them 222 cases were male, only 1 female(HA), 177 cases were from Rural areas (79.4%).

[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].

Aim: To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.

Methods: The prokaryotic expression vector pQEN containing N gene was constructed and transformed into the E.

[Prokaryotic expression of SARS coronavirus spike protein and construction of its DNA vaccine].

Objective: To study the immunological characteristics of the spike (S) protein of SARS coronavirus (SARS-CoV) and analyze the feasibility of using this protein as the component for SARS vaccine development.

Methods: The two truncated fragments of S gene were separately cloned into the prokaryotic expression vector pET-15b and expressed in E.coli.