[Chemiluminescent detection of E. coli O157:H7 in food based on magnetic enzyme-linked immuno method].

Aim: To establish a new chemiluminescent assay with high sensitivity for detecting E. coli O157:H7 in food.

Methods: The assay was established based on the alkaline phosphatase (ALP) labeled anti-E.

[A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin].

Aim: To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.

Methods: Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.