Biosens Bioelectron
October 2024
The progression of gastric cancer involves a complex multi-stage process, with gastroscopy and biopsy being the standard procedures for diagnosing gastric diseases. This study introduces an innovative non-invasive approach to differentiate gastric disease stage using gastric fluid samples through machine-learning-assisted surface-enhanced Raman spectroscopy (SERS). This method effectively identifies different stages of gastric lesions.
View Article and Find Full Text PDFZhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
August 2016
Objective: To survey on Toxoplasma gondii infection in depressed patients in Guizhou Province and identify the genotype of T. gondii.
Methods: Enzyme-linked immunosorbent assay (ELISA) was applied to detect the T.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
April 2015
Blood samples were collected from patients with leukemia (n = 150) or lymphoma (n = 150) in the Cancer Hospital from March to September 2014. The specific antibodies (IgG, IgM) to, and circulating antigens (CAg) of Toxoplasma gondii were determined by ELISA. A 529 bp specific sequence was amplified by PCR from the genomic DNA of T.
View Article and Find Full Text PDFBackground: Toxoplasma gondii is an obligate, intracellular protozoan that infects almost all warm-blooded animals, including humans, domesticated and wild animals. Recent studies of Toxoplasma gondii isolates from animals in different regions of China have shown a limited genetic diversity with the dominance of the ToxoDB PCR-RFLP genotype #9 named as "Chinese 1". However, there is not much published information regarding its prevalence in domestic animals from Guizhou province, a subtropical region in Southwest China.
View Article and Find Full Text PDFHBV covalently closed circular DNA (cccDNA) is the template for the transcription of HBV. HBV core protein (HBc) is a main component of the HBV cccDNA minichromosome. However, the function of HBc in cccDNA is not fully understood.
View Article and Find Full Text PDFStereotact Funct Neurosurg
May 2011
Background: Numerical processing is important in our everyday lives. However, very few attempts have been made to map the numerical processing function areas during lesion surgery.
Objective: To identify and protect the cortical areas involved in numerical processing, the authors used the intraoperative brain mapping approach to study numerical processing areas in patients with parietal lobe tumors.
The reaction of cis-[Pt(NO(3))(2)(NH(3))(2)] and sodium glycolate yielded the title compound, [Pt(C(2)H(2)O(3))(NH(3))(2)]. The Pt(II) atom, coordinated by two N atoms of ammine and two O atoms of the carboxyl-ate and oxido groups of the glycolate ligand, is in a square-planar environment. In the crystal structure, mol-ecules are connected by inter-molecular N-H⋯O hydrogen bonds, forming a three-dimensional network.
View Article and Find Full Text PDFZhongguo Zhong Xi Yi Jie He Za Zhi
March 2007
Objective: To investigate the effects of Ginkgo biloba extract (Ginaton) on bcl-2 and bcl-xL mRNA expression in the myocardium of patients underwent hypothermic cardiopulmonary bypass (CPB).
Methods: Thirty congenital heart disease patients were randomly assigned to 2 groups, the control group and the treated group. Patients in both groups received St.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
December 2004
Objective: To study the mechanism of hepatitis C virus (HCV) gene regulation and the inhibitory effect of antisense RNA on HCV gene expression in vitro.
Methods: The hepatoblastoma cell line (HepG2) was co-transfected by recombinant plasmid of antisense RNA complementary to HCV 5' untranslated region (5'UTR)and HCV 5' UTR Directed luciferase (luc) gene expression recombinant plasmid. Meanwhile a reversed HCV 5'UTR recombinant plasmid which can not transcribe as antisense RNA in the cell and a recombinant plasmid in which the luc was regulated by simian virus 40 (sv40) 5'UTR were used as controls respectively.
The frequency of Y-chromosome haplotypes consisted of thirteen single nucleotide polymorphism alleles in Bouyei people was investigated using PCR-RFLP. And the polymorphism of mitochondrial DNA Region V was studied. Nine Y-chromosome haplotypes (H1, H4, H5, H6, H7, H8, H9, H11, H12) and two mtDNA alleles (9-bp deletion) were obtained in the samples.
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