[Effect of emodin lipid nano-microbubble on MAPK signal pathway and inflammation cytokine in AT-II cells by mechanical stretch].

Objective: To investigate the effect of emodin lipid nano-microbubble on MAPK signaling pathway and the level of inflammation cytokine in AT-II cells induced by mechanical stretch and its mechanism.

Methods: Emodin nanofibers, prepared by electrospinning with lecithin and PVP as carrier, were put into a seal bottle full of perfluoropropane, after they mixed with water and turned into self-assembled nano-microbubble. AT-II cells, separated and purified from primary rat AT-II cells, suffered 20% mechanical stretch for 4, 8, 16, 24, 48 h, and its protein expressions of p-P38/P38, p-ERK/ERK, p-JNK/JNK and the inflammation cytokine release levels of TNF-alpha, IL-1beta, IL-6 were detected by Western-Blot and ELISA.

Electrospun drug-loaded core-sheath PVP/zein nanofibers for biphasic drug release.

Core-sheath nanofibers prepared using coaxial electrospinning were investigated for providing biphasic drug release profiles. With ketoprofen (KET) as the model drug, polyvinylpyrrolidone and zein as the sheath polymer and core matrix, respectively, the coaxial process could be carried out smoothly and continuously without any clogging of the spinneret. Scanning electron microscopy and transmission electron microscopy observations demonstrated that the nanofibers were linear with homogeneous structure and had a clear core-sheath structure with an average diameter of 730 ± 190 nm, in which the sheath had a thickness of ca.

[Gene transfer system mediated by PEI-cholesterol lipopolymer with lipid microbubbles].

The properties of polyethyleneimine-cholesterol cationic lipopolymer (PEI-Chol) as gene carries and its gene transfer efficiency in vitro with lipid microbubbles were presented in this paper. PEI-Chol lipopolymer was synthesized by linking cholesterol chloroformate to the amino groups of branched poly(ethyleneimine) (PEI) of 1 800. The structure and molecular weight of PEI-Chol were confirmed by IR, 1H NMR and MADI-TOF-MS (matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry), respectively.

[Preparation of self-emulsifying soft capsule and its pharmacokinetic in rats for epimedium flavonoids].

Objective: To study the formulation optimization of epimedium flavonoids self-emulsifying drug delivery system and evaluate its effect in vitro and in vivo.

Methods: Based on the degree of emulsification and emulsifying time, the formulation optimization (i.e.

[Effects of Tankejing dry powder inhaler on the inflammatory damage in the lungs of mice infected by FM1].

Objective: To study the effect of Tankejing Dry Powder inhaler on inflammatory in lung injury of mice infected by influenza virus (FM1).

Methods: Model mice infected by influenza virus FM1 were randomly divided into six groups: the nomal group, model group, low dose, medium dose and high dose of Tankejing Dry Powder inhaler group,and Ribavirin group. The following indices including the change of NF-kappaB in lung tissue at 24 h, the changes of TNF-alpha, IL-1beta, IL-6 in lung tissue, the lung index and the death rate were observed.

[Anti-viral effects of Tankejing preparations against influenza virus A].

Objective: To investigate the anti-viral effects of Tankejing preparations (including solutions, dry power inhalation and powder) against influenza virus A in vitro and the relationship between its anti-viral effect and preparations.

Methods: The inhibitive effect of different drug-added ways of Tankejing extracts against human influenza virus A (H3N2) in vitro were assayed by crystallized purple staining method with ribavirin as positive reference drug. Then we compared the anti-viral actions of different kinds of Tankejing preparations.

[Effects of epimedium total flavonoids phytosomes on preventing and treating bone-loss of ovariectomized rats].

Objective: To observe the effects of Epimedium total Flavonoids Phytosomes on preventing and treating bone-loss of the castrate osteoporosis rat model.

Method: The osteoporosis model was established with 4-month-odl panther's rats, their ovaries on both sides castrated. Dual energy X-ray scanning was used to determine the bone density, and immunity and ELASA were used to assay concentration of estradiol and IL-6 in serum respectively, then determine their effect.