Publications by authors named "Yong-ming Fang"
[Construction of eukaryotic expression plasmids containing green fluorescent protein gene and CYP19 WT or its variants].
Zhejiang Da Xue Xue Bao Yi Xue Ban
March 2011
Objective: To construct eukaryotic expression plasmids containing green fluorescent protein gene and CYP19 wild-type or its variants (W39R, R264C, W39R-R264C) and to observe its expression in MCF-7 and Bcap-37 cells.
Methods: The aromatase WT cDNA sequence was obtained by RT-PCR amplification and cloned into the eukaryotic expression vector pcDNA3.1(+).
Background: There is much variation in the response of both individuals and different ethnic populations to opioids, with genetic differences being responsible for interindividual variation. The micro-opioid receptor single nucleotide polymorphism (rs number 1799971) at nucleotide position 118 (OPRM1 118A>G) affects the analgesic response to opioids.
Objective: This study aimed to investigate the association between the OPRM1 118A>G polymorphism and the effects of fentanyl-induced analgesia, respiratory depression, and anesthetic recovery responses in a population of Han Chinese patients.
Aim: To investigate the expression of SNC73, a trans-cript of the immunoglobulin alpha-1 gene (IgA1-H chain), in human epithelia-derived tumor cells.
Methods: Total RNAs and cell lysates were prepared from five different human epithelial cell lines derived from lung, stomach, liver, skin, and breast, respectively. RT-PCR and immunoblot analysis of these five cell lines were done.
Objective: To investigate the mechanism of apoptosis of chronic myeloid leukemia (CML) cells induced by the novel p210 bcr/abl inhibitor berbamine.
Methods: Human Ph+ CML leukemia K562 cells, which express endogenous p210 bcr/abl protein, were cultured in RPMI 1640 and treated with berbamine as indicated time and dose. Flow cytometry (FCM) and Annexin-V-Fluos/PI staining kit were used to evaluate the apoptosis of leukemic cells; FCM and cytoperm/cytofix plus Caspase-3-McAb-PE were employed to measure the leukemic cells with activated Caspase-3.
Article Synopsis
- - The study aimed to assess the levels of Shp-2 tyrosine phosphatase in chronic myeloid leukemia (CML) and how it relates to the cancer cells' ability to grow uncontrollably and resist apoptosis.
- - Researchers collected samples from 25 CML patients alongside healthy control samples to evaluate Shp-2 expression using Western blotting and monitored cell apoptosis through flow cytometry after manipulating Shp-2 levels.
- - The findings showed that phosphorylated Shp-2 was significantly overexpressed in 92% of CML samples compared to normal cells, indicating a potential link to the malignancy's aggressive growth and survival characteristics.
[Induction of HSF1 expression and sporadic colorectal cancer].
Zhejiang Da Xue Xue Bao Yi Xue Ban
September 2004
Objective: To explore the activation of the signal transduction pathways related with the carcinogenesis of sporadic colon cancers.
Methods: A gene microarray monitoring activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissue. The differentially expressed genes were further analyzed by RT-PCR, using RNA extracted from cancer tissue and matched normal colon mucosa of 35 patients with colorectal cancer.
Induction of HSF1 expression is associated with sporadic colorectal cancer.
World J Gastroenterol
November 2004
Aim: To explore the activation of signal transduction pathways related with the carcinogenesis of sporadic colon cancers.
Methods: A gene array monitoring the activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissues. The differentially expressed genes were further analyzed by RT-PCR, using RNA derived from colorectal cancer and normal colon tissue of 35 patients.