Transfect bone marrow stromal cells with pcDNA3.1-VEGF to construct tissue engineered bone in defect repair.

Background: We previously showed that nano-hydroxyapatite/carboxymethyl chitosan (n-Ha/CMCS) displayed excellent mechanical properties, good degradation rates and exceptional biocompatibility, with negligible toxicity. The aim of this study was to determine the effect of the same composite with vascular endothelial growth factor (VEGF)- transfected bone marrow stromal cells (BMSCs) in a rabbit radial defect model.

Methods: The nano-hydroxyapatite was produced through co-precipitation.