Pituitary tumor-transforming gene 1 regulates invasion of prostate cancer cells through MMP13.

It is critical to understand the molecular mechanisms underlying the migration and invasiveness of prostate cancer (PC) for improving the outcome of therapy. A relationship of pituitary tumor-transforming gene 1 (Pttg1) and matrix metalloproteinase 13 (MMP13) in PC as well as their roles in the metastases of PC has not been studied. Here, we reported significantly higher levels of Pttg1 and MMP13 in the resected PC specimens, compared to the adjacent normal prostate tissue from the same patient.

[Prognostic value of PSA kinetics in locally advanced prostate cancer treated by maximal androgen blockade combined with brachytherapy].

Objective: To evaluate the effect of post-treatment PSA kinetics on the prognosis of prostate cancer (PCa).

Methods: We retrospectively reviewed the clinical data of 114 cases of locally advanced PCa treated by maximal androgen blockade (MAB) combined with brachytherapy, and analyzed the association of the changes in PSA kinetics with the prognosis of the patients.

Results: The median survival time of the patients was 81 (15 - 144) months, with 1-, 3- and 5-year survival rates of 91.

[Clinical study of congenital heart disease accompanied by hypospadias].

Objective: To study the concurrence of congenital heart disease and hypospadias and the relationship between the two diseases.

Methods: We investigated the incidence and types of congenital heart disease accompanied by hypospadias in male children received in our hospital from January 2002 to December 2012, compared them with those in the general population, and analyzed the correlation of different types of heart disease with the incidence rate of hypospadias.

Results: Of the 7 385 male children with congenital heart disease, 134 (1.

[Long-term outcomes of prostate cancer patients treated with combined brachytherapy and maximal androgen blockade].

Objective: To explore the prognostic factors of prostate cancer (PCa) patients and evaluate the effect of brachytherapy on survival time.

Methods: A total of 289 PCa were recruited to collect their clinical and survival data. And their possible prognostic factors were analyzed.

Epithelial-mesenchymal transition and migration of prostate cancer stem cells is driven by cancer-associated fibroblasts in an HIF-1α/β-catenin-dependent pathway.

Although cancer stem cells (CSCs) play a crucial role in seeding the initiation of tumor progression, they do not always possess the same potent ability as tumor metastasis. Thus, precisely how migrating CSCs occur, still remains unclear. In the present study, we first comparatively analyzed a series of prostate CSCs, which exhibited a dynamically increasing and disseminating ability in nude mice.

[Epithelial-mesenchymal transition of prostate cancer: cancer stem cells or bulk cancer cells].

Objective: To test the hypothesis that epithelial-mesenchymal transition (EMT) of prostate cancer is most likely to occur in cancer stem cells (CSC).

Methods: The isolation of CSC from LNCaP cell line was performed by flow cytometry based on side-population (SP) phenotype. After SP sorting, LNCaP/SP and LNCaP/NSP were used for further transfection of hypoxia-inducible factor-1α (HIF-1α).

[Sorting and identification of cancer stem cells in human prostate cancer cell lines].

Objective: To sort and identify side population (SP) cancer stem cells (CSC) in human prostate cancer (PCa) cell lines.

Methods: Stem-like cells were isolated from five PCa cell lines Du145, IA8, LNCaP, TSU-Pr and PC-3 using FACS based on CD133+ CD44+ immunophenotype and SP in Hoechst staining. The in vitro growth pattern and tumorigenicity of SP stem cells were verified by soft agar colony-formation trial.

[Factors influencing finasteride medication compliance in outpatients with benign prostatic hyperplasia].

Objective: To investigate the factors influencing the compliance, discontinuation and switching of finasteride medication in patients with benign prostatic hyperplasia (BPH).

Methods: We retrospectively analyzed the electronic clinical data of 655 outpatients with BPH treated with finasteride from January 2008 to June 2010. Using the medication possession ratio (MPR), we measured their medication compliance and the rates of discontinuation and switching after an average observation of 12 months.

Finasteride adherence-associated factors in Chinese benign prostatic hyperplasia patients.

Objectives: Medication noncompliance is a recognized problem worldwide. This study evaluated the factors associated with compliance, discontinuation and switching of finasteride among Chinese benign prostatic hyperplasia patients.

Materials And Methods: A retrospective cohort study was conducted with 682 outpatients newly diagnosed with benign prostatic hyperplasia and prescribed with finasteride from January 2008 to December 2009, taken from a database.

Knockdown of β-Catenin through shRNA cause a reversal of EMT and metastatic phenotypes induced by HIF-1α.

Objective: Wnt/β-catenin signaling pathway regulates pattern formation during embryogenesis as well as tumor progression. Numbers of studies suggest that this signaling pathway may play an important role in Epithelial-Mesenchymal transition (EMT), however, there was no evidence that Wnt/β-catenin signaling pathway directly controlled the EMT occurrence. Our previous research has successfully proved that overexpression of hypoxia inducible factor-1α (HIF-1α) could induce EMT in LNCaP cells, but not in PC-3.

[Characterization of prostate cancer cell lines and their epithelial-mesenchymal transition in subcutaneous tumors].

Objective: To identify the differences in the expression of epithelial or mesenchymal standard proteins between prostate cancer cell lines and tumors, and to investigate the relationship between the process of the prostate cancer cell line forming subcutaneous tumors and epithelial-mesenchymal transition (EMT) by comparing the characteristics of different prostate cell lines forming subcutaneous tumors in SCID mice.

Methods: We constructed prostate cancer models in male SCID mice by subcutaneous injection of 4 human prostate cancer cell lines DU145, Tsu, PC3 and LNCaP, and compared the characteristics of tumor formation. We used Western blot to detect the expressions of E-cadherin and Vimentin in the cancer cell lines and subcutaneous tumors, observed their differences before and after tumor formation, and explore the relationship between EMT and tumor formation.

[Expression of COX-2 in different human prostate cancer cell lines and its significance].

Objective: To investigate the expression of cyclooxygenase-2 (COX-2) in different prostate cancer (PCa) cell lines and its role in the acquisition of invasive and metastatic potentials of PCa.

Methods: We detected the expressions of COX-2 in prostate cancer cell lines LNCaP, C4-2, IF11, IA8 and PC-3 with different metastatic potentials by Western blotting and RT-PCR, and analyzed their roles in the invasion and metastasis of different PCa cell lines.

Results: Western blotting and RT-PCR showed that the expression of the COX-2 protein was high in PC-3, but absent in IF11, IA8, LNCaP and C4-2 (P < 0.

Effects of prostate manipulation on serum total and free prostate specific antigen, and free-to-total prostate specific antigen ratio.

Objective: To evaluate the effects of the different types of manipulation on prostate total specific antigen (tPSA), free prostate specific antigen (fPSA), and free-to-total prostate specific antigen (f/tPSA).

Methods: A total of 160 males were enrolled from January 2006 to December 2009 in the Urology Department, Beijing Anzhen Hospital affiliated to the Capital Medical University, Beijing, China. Of these patients, 23 had digital rectal examination (DRE), 21 had urethral catheterization, 28 had rigid cystoscopy, 35 had prostate biopsy, 35 underwent transurethral resection of the prostate (TURP), and 18 underwent suprapubic prostatectomy.

[Role of beta-catenin signaling pathway in EMT of human prostate cancer induced by HIF-1alpha].

Objective: Epithelial-mesenchymal transition (EMT) is an important process in tumor development. Several studies suggest that the beta-catenin signal pathway may play an important role in EMT. However, there is no direct evidence showing that this pathway actually determines the EMT induced by exogenous signal.

[Epididymal cyst in von Hippel-Lindau syndrome: clinicopathologic analysis of 3 cases].

Objective: To investigate the clinicopathologic features of epididymal cyst in von Hippel-Lindau (VHL) syndrome.

Methods: We reviewed the clinical data of 3 epididymal cyst patients treated by surgery, and detected the expressions of HIF-1alpha, VEGF, alpha-SMA and CD34 in the epididymal tissue samples by the immunohistochemistry SP method.

Results: All the 3 patients underwent surgical removal of the epididymal cyst.

[Expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and their significance].

Objective: To observe the expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and explore their relationship with the metastasis of human prostate cancer.

Methods: The expressions and distribution of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines (IF11 and IA8) were detected by Western blot and immunofluorescent staining.

Results: The expression of E-cadherin was high in LNCaP, but absent in IF11 and IA8, while beta-catenin was expressed highly in IF11 and LA8, but lowly in LNCaP.

Eighty-six cases of laparoscopic vaginoplasty using an ileal segment.

Background: The surgical management of the absence of the vagina is a complex problem and constitutes a significant technical challenge. As the laparoscopy has been an important tool for the treatment of uterovaginal anomalies, we evaluated the feasibility of laparoscopic vaginoplasty using an ileal segment retrospectively.

Methods: Totally 86 patients who underwent laparoscopic vaginoplasty using an ileal segment in Beijing Anzhen Hospital during February 2004 to July 2007 were enrolled in this study.

Laparoscopic vaginal reconstruction using an ileal segment.

Objective: To evaluate the feasibility of laparoscopic vaginal reconstruction using an ileal segment in patients with vaginal atresia, male-female transsexual patients, or patients who had vaginal cancer.

Methods: A retrospective review of the records of 80 patients who underwent laparoscopic vaginoplasty between 2004 and 2007 at Beijing Anzhen Hospital, China.

Results: Nineteen (23.

[Identification of Wnt/beta-catenin signal pathway in human prostate cancer cell lines and its significance].

Objective: To determine the Wnt/beta-catenin signal pathway in different human prostate cancer cell lines and explore its role in epithelial-mesenchymal transition (EMT) in human prostate cancer.

Methods: We detected the expressions of beta-catenin, t-GSK3beta and p-GSK3beta in several prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B, IF11, IA8, PC-3 and DU145) with different characteristics of epithelial-mesenchymal transition (EMT) by Western blotting.

Results: There were remarkable differences in the expressions of beta-catenin and p-GSK3beta among the cell lines, with beta-catenin and p-GSK3beta highly expressed in LNCaP, C4, C4-2, C4-2B, IF11 and IA8, lowly expressed in PC-3 and DU145, but no difference was observed in the expressions of t-GSK3beta in all the cell lines.

[Finasteride: an effective therapeutic for benign prostatic hyperplasia related gross hematuria in patients receiving anticoagulant].

Objective: To study the effect of finasteride on benign prostatic hyperplasia (BPH) related gross hematuria in patients receiving anticoagulant.

Methods: A total of 105 patients with BPH related gross hematuria were divided into an anticoagulant group (n = 81), treated with combined therapy of anticoagulants and finasteride, and a control group (n = 24), given finasteride only at 5 mg daily. The therapeutic effects were compared by a 6-month follow-up.

[Loss of heterozygosity on chromosome 10 is associated with bone metastasis of human prostate cancer].

Objective: To study the cytogenetic mechanism of bone metastasis of human prostate cancer (PCa).

Methods: We analyzed chromosome variation by comparative genomic hybridization in 18 patients with prostate cancer to determine the chromosome variants associated with bone metastasis, and focused on 7 microsatellite sites on chromosome 10 for the detection of the loss of heterozygosity (LOH) by PCR-based microsatellite polymorphism analysis.

Results: In the 11 samples with bone metastasis, the variation rate of chromosome 10 was 90.

[Over-expression of HIF-1alpha induces EMT of human prostate cancer cells].

Objective: To determine whether human prostate cancer cell lines undergo epithelial-mesenchymal transition (EMT) and become more invasive when induced by HIF-1alpha, and to explore the underlying molecular mechanism.

Methods: The cell line LNCaP, appropriate for the HIF-1alpha induction test, was screened out from 4 different EMT-negative prostate cell lines through vimentin gene detection by RT-PCR. The recombinant plasmid pCDNA3.

Resveratrol reestablishes spermatogenesis after testicular injury in rats caused by 2, 5-hexanedione.

Background: Environmental toxins can destroy the physiological process of spermatogenesis and even lead to male infertility. Resveratrol (RES) is a natural phytoalexin with a wide range of biological activities. Some recent researches have demonstrated that RES can increase sperm output and protect sperm from apoptosis caused by physical damage.

[Expression of coxsackie B virus-adenovirus receptor in prostate cancer Du145 and LNCaP cell lines and its significance].

Objective: To observe the expression of Coxsackie B virus-adenovirus receptor (CAR) in two prostate cancer cell lines with different metastatic potentials.

Methods: The expressions of CAR in two prostate cancer cell lines (Du145 and LNCaP) with different metastatic potentials were detected by Western blotting. The Transwell polycarbonate filter was used to analyze the invasive potency.

Role of Wnt/beta-catenin signaling pathway in epithelial-mesenchymal transition of human prostate cancer induced by hypoxia-inducible factor-1alpha.

Objectives: Epithelial-mesenchymal transition (EMT) is an important process in tumor development, and several studies suggest that the Wnt/beta-catenin signal pathway may play an important role in EMT. However, there is no direct evidence showing that the Wnt/beta-catenin pathway actually determines the EMT induced by an exogenous signal. Our previous research has successfully proved that overexpression of hypoxia-inducible factor-1alpha (HIF-1alpha) could induce EMT in LNCaP cells, but not in PC-3.