[Comparison of ELISA diagnostic kits used in China for hepatitis C virus IgG antibody detection].

Objective: To compare the commercial diagnostic reagent available in China for hepatitis C virus ( HCV) IgG antibody detection in order to provide some useful information for HCV prevention.

Methods: The HCV-IgG detection reagents produced by six different Enterprises named A, B, C, D, E and F were chosen and applied to detect 160 HCV specious sera samples. HCV-IgG reagent from ABBOTT was adopted as gold-standard and the samples in gray zone were determined by RIBA method finally.

[Hepatitis C viruses infection situation in the human population of six provinces in China].

Objective: To investigate the seroprevalence of hepatitis C viruse infection in the human population in six regions of Beijing, Heilongjiang, Shandong, Ningxia, Gansu and Sichuan in China.

Methods: ELISA was used for detecting anti-HCV IgG of the serum samples. All sample were collected in 2006-2008 in six areas.

[Hepatitis viruses infection situation in human population of the Gansu province].

Objective: To investigate the seroprevalence of hepatitis viruses in human population of Ganso province.

Methods: ELISA was used for detecting anti-HAY IgG, HBsAg/HBsAb, anti-HCV IgG and anti-HEV IgG of the serum samples. All sample were collected in four areas of KL, LT, HN and ZhL of Gansu province in 2008.

[Antibody detection of hepatitis E virus in some human population, swine and chicken in Beijing in China].

Objective: To investigate the seroprevalence of HEV infection in human population, swine and chicken in Beijing region.

Methods: EIA was used for detecting anti-HEV IgG of the serum samples. All samples were collected in 2006-2007 in Beijing areas.

[Antibody detection and sequencing analysis of hepatitis E virus in human population, swine and chicken in Sichuan region in China].

Objective: To investigate the seroprevalence of HEV infection and genotype.

Methods: ELISA were used for detecting anti-HEV IgG of the serum samples, the nested reverse transcriptase PCR (RT-nPCR) was used for detecting HEV RNA in patient serum and swine bile samples. All samples were collected in 2005-2007 in some districts in Sichuan province.

[Hepatitis viruses infection situation in Mianyang of the Sichuan province].

Objective: To investigate the seroprevalence of hepatitis viruses in Mianyang of the Sichuan province.

Methods: EIISA was used for detecting anti-HAV IgG, HBsAg/HBsAb, anti-HCV IgG and anti-HEV IgG of the serum samples. All sample were collected in Mianyang areas in 2007.

[Evaluation of the ELISA diagnostic kits for hepatitis E virus antibody in the reference serum, the suspect patients of hepatitis E and normal persons' sera].

Objective: To evaluate the quality of the ELISA diagnostic kits for detecting hepatitis E virus (HEV) specific IgG antibody.

Methods: Five diagnostic kits (WT, GD, HM, GeneLabs and KH) for anti-HEV IgG were assayed by detecting HEV IgG in the HEV diagnostic reference sera from 24 positive cases and 30 negative cases. 42 cases clinical suspect patients with hepatitis E from Ditan hospital in Beijing in 1994 to 1995 and 230 normal persons' sera from Chengdu city, Sichuan province in September in 2005.

[Expression of the hepatitis Delta antigen in prokaryotic cell and evaluation of its application as an EIA diagnostic reagent].

Background: To construct the pRSETB-HDAg recombinant expression plasmid and to obtain soluble hepatitis D virus antigen with high biological and antigenic activity.

Methods: HDAg gene fragment was inserted into fusion expression pRSET B vector that includes T7 promoter and a polyhistidine tag. The recombinant plasmid was transformed into host bacterium BL21 after induction with IPTG.

[Antigencity identification of recombinant hepatitis E virus ORF2 protein expressed in Pichia pastoris].

Background: To determine the antigenicity of recombinant hepatitis E virus ORF2 (rHEV ORF2) protein expressed in Pichia pastoris (P. pastoris).

Methods: By using the rHEV ORF2 protein from E.

Detection of anti-MHAV and anti-MHEV IgM in patients with sporadic acute hepatitis in Beijing between 1995-2000.

Objective: To investigate the age range, liver function damage and prognosis of patients with sporadic acute hepatitis A and E in Beijing.

Methods: Enzyme immunoassay (EIA) was used to detect anti-HAV and anti-HEV immunoglobulin M (IgM). Serum samples were collected from the patients with sporadic acute viral hepatitis in Beijing from January 1995 to June 2000.