[Caudal-related homeobox 2 gene expression and its effects on human gastric carcinoma cell line].

Objective: To investigate the effect of human caudal-related homeobox 2 (Cdx2) gene expression on human gastric carcinoma cell line MGC-803.

Methods: pCMV-Cdx2-HA eukaryotic expression plasmid was constructed by using DNA recombinant method. The MGC-803 cells were divided into 4 groups: non-transfected, transfected with pCMV-HA, transfected with pCMV-GAPDH-HA, transfected with pCMV-Cdx2-HA.

[Inhibitory effects of transcription factor E2F-1 siRNA on invasion and proliferation of gastric cancer cell line MGC803].

Background & Objective: As an important regulatory factor of cell cycle, transcription factor E2F-1 is closely related to tumorigenesis. This study was to investigate the effects of E2F-1 small interfering RNA (siRNA) on invasion and proliferation of human gastric cancer MGC803 cells.

Methods: E2F-1 siRNA vector containing short hairpin structure was transfected into MGC803 cells.