Fast Imaging of Mitochondrial Thioredoxin Reductase Using a Styrylpyridinium-Based Two-Photon Ratiometric Fluorescent Probe.

Thioredoxin reductase (TrxR) is a pivotal antioxidant enzyme, but there remains a challenge for its fast imaging. This work describes the combination of a hydroxyl styrylpyridinium scaffold as the push-pull fluorophore with a carbonate-bridged 1,2-dithiolane unit as the reaction site to develop a fast mitochondrial TrxR2 probe, . It manifested a plethora of excellent properties including a rapid specific response (12 min), large Stokes shift (170 nm), ratiometric two-photon imaging, favorable binding with TrxR ( = 12.

Developing Push-Pull Hydroxylphenylpolyenylpyridinium Chromophores as Ratiometric Two-Photon Fluorescent Probes for Cellular and Intravital Imaging of Mitochondrial NQO1.

This work highlights the use of push-pull hydroxylphenylpolyenylpyridinium fluorophores coupled with trimethyl lock quinone to engineer the ratiometric two-photon probes for cellular and intravital imaging of mitochondrial NAD(P)H:quinone oxidoreductase 1 (NQO1), a critical antioxidant enzyme responsible for detoxifying quinones. As a typical representative, showed favorable binding with NQO1 with a Michaelis constant of 12.74 μM and exhibited a suite of superior properties, including rapid response (4 min), large Stokes shift (162 nm), ultralow detection limit (0.

[Sources, Distribution of Main Controlling Factors, and Potential Ecological Risk Assessment for Heavy Metals in the Surface Sediment of Hainan Island North Bay, South China].

The distribution characteristics of the content of As, Cr, Cu, Hg, Pb, Zn, and Cd were researched based on the geochemical analysis of 159 surface sediment samples in Haikou Bay, Puqian Bay, Dongzhai Harbor, and Mulan Bay Mathematical statistics methods were used to interpret the sources of heavy metals, and the main controlling factors for heavy metal distribution were confirmed, having been analyzed qualitatively and semi-quantitatively. The results showed that the average contents of As, Cd, Cr, Cu, Hg, Pb, and Zn were 8.40, 0.