[Effect of superantigen SEA on the CD3epsilon chain expression on cord blood mononuclear cells stimulated by K562 cells].

Aim: To explore the effect of staphylococcal enterotoxin A(SEA) on CD3(epsilon) chain expression on mononuclear cells in cord blood, which were stimulated by K562 cells.

Methods: The anti-CD3 antibodies(mAb), K562 cells, SEA or both SEA and K562 cells were cocultured with mononuclear cells (MNCs) from four normal human cord blood for 48 hours respectively, Real-time PCR with SYBR Green I technique was used to detect expressed level of CD(epsilon) on MNCs in cord blood and set up a blank control group. Relative changes in expression level of CD3(epsilon) chain were indicated by the 2(-deltadeltaCt); method between each group and the control group.