Chromophorylation of a Novel Cyanobacteriochrome GAF Domain from and Its Response to Copper Ions.

Cyanobacteriochromes (CBCRs) are phytochrome-related photoreceptor proteins in cyanobacteria and cover a wide spectral range from ultraviolet to far-red. A single GAF domain that they contain can bind bilin(s) autocatalytically via heterologous recombination and then fluoresce, with potential applications as biomarkers and biosensors. Here, we report that a novel red/green CBCR GAF domain, SPI1085g2 from , covalently binds both phycocyanobilin (PCB) and phycoerythrobilin (PEB).

Fluorescence Properties of a Novel Cyanobacteriochrome GAF Domain from that Exhibits Moderate Dark Reversion.

Cyanobacteriochromes (CBCRs) are biliproteins for photoreception that are present in cyanobacteria. These proteins possess one or more unique cGMP-specific phosphodiesterase/adenylate cyclase/FhlA (GAF) domains that can covalently bind the linear tetrapyrrole (bilin). Light absorption triggers the photoisomerization of bilin between the 15Z and 15E photostates.

[Responses of CO2 fluxes to light intensity and temperature in rice paddy field].

CO2 fluxes in rice paddy ecosystem in subtropical hilly region were measured continuously using eddy covariance technique. The objectives were to investigate the responses of CO2 fluxes to light intensity and temperature in the paddy ecosystem. Results showed a rectangular hyperbolic light-response function could be used to describe the relationship of CO2 flux and photosynthetic photon flux density (PPFD).

[Seasonal characteristics of CO2 fluxes from the paddy ecosystem in subtropical region].

CO2 fluxes from paddy ecosystem in subtropical hilly region were measured continuously using eddy covariance technique. Based on data rejecting, correcting and filling, the daily and annual CO2 fluxes were calculated from the instantaneous values, respectively. The objectives were to investigate the variation of CO2 fluxes on seasonal temporal scale, analyze the relationship between CO2 fluxes and environmental factors, and to quantify the annual net ecosystem exchange (NEE) from the paddy ecosystem.

[Estimation of CO2 fluxes from rice paddies based on transparent chamber measurement].

Closed chamber provide a valuable tool for measuring CO2 exchange fluxes. In general, the change rate of CO2 concentration is assumed to be constant in the short measurement time and a linear regression method is used to estimate the CO2 fluxes. However, due to the physical and physiological effects, the change rate of CO2 is not always constant.