Melting Away Pain: Decay of Thermal Nociceptor Transduction during Heat-Induced Irreversible Desensitization of Ion Channels.

Thermal transient receptor potential channels play a key role in thermal sensation. Although predictive models exist for temperature-dependent transduction through these channels and for the associated sensations of pain, the ability to predict irreversible desensitization has been lacking. We explored the role of irreversible ion channel desensitization in pain sensation and hypothesized that desensitization of ion channels would follow the kinetics similar to the denaturation of catalytic enzymes.

Ox-LDL induces dysfunction of endothelial progenitor cells via activation of NF-κB.

Dyslipidemia increases the risks for atherosclerosis in part by impairing endothelial integrity. Endothelial progenitor cells (EPCs) are thought to contribute to endothelial recovery after arterial injury. Oxidized low-density lipoprotein (ox-LDL) can induce EPC dysfunction, but the underlying mechanism is not well understood.

Factors associated with decision time for patients with ST-segment elevation acute myocardial infarction.

Increased delay in visiting a hospital for patients with ST-segment elevation myocardial infarction (STEMI) is often associated with poor outcomes. The factors associated with the decision time were analyzed by comparing the characteristics of patients with delays longer or shorter than the median of 60 min. Pre-hospital delay tended to be longer for patients living in suburban areas compared to those in urban areas (P=0.

[The change of transient receptor potential vanilloid-1 and interleukin-1α in periodontal tissue during experimental tooth movement].

Purpose: To investigate the expression of TRPV1 and IL-1α in Sprague-Dawley rats' periodontal ligament (PLD) during experimental tooth movement of different orthodontic force and duration, and explore the role of TRPV1 and IL-1α in orthodontic pain.

Methods: 66 Sprague-Dawley rats were divided into control group (n=6), sham operation group (n=6) and experimental group (n=54) randomly. Orthodontic force was applied on right first maxillary molar in rats and the changes of TRPV1 and IL-1α expression were detected by real-time PCR at 4 h, 8 h, 1 d (three subgroups were added according to different forces: 1 d-30 g, 1 d-50 g, 1 d-80 g ), 3 d, 5 d, 7 d, 14 d after tooth movement.

[The change of calcitonin gene- related peptide in periodontal tissue during orthodontic tooth movement].

Purpose: To establish a Sprague-Dawley rat model of tooth movement, detect the expression of calcitonin gene-related peptide (calcitonin gene related peptide, CGRP) in Sprague-Dawley rats' periodontal tissues during tooth movement and explore the role of the peripheral mechanisms of pain in orthodontics.

Methods: 60 Sprague-Dawley rats were randomly divided into experimental group (n=54) and control group (n=6). Force was applied on right maxillary first molar in rats and the changes of CGRP expression were detected by real-time PCR 4 h, 8 h, 1 d, 3 d, 5 d, 7 d, 14 d after tooth movement.

[Construction and identification of BIRC5 shRNA lentiviral expression vector].

Aim: Design and synthesis complementary DNA sequences of 3 pairs of short hairpin structure and a pair of negative control sequence by RNA interference technique and construct and identify a lentiviral interference vector with human BIRC5 gene as target gene.

Methods: The designed and synthesised Single-Stranded primer were annealed to Double-Stranded oligo sequences and subcloned into linear pMAGic lentiviral plasmid vector digested by enzyme Age I and EcoR I. Screening positive clone after transformed into DH5α competent cells and identified by PCR amplification and DNA sequencing.

H(1) and H(2) receptors in the locus ceruleus are involved in the intracerebroventricular histamine-induced carotid sinus baroreceptor reflex resetting in rats.

Objective To investigate the role of H(1) and H(2) receptors in the locus ceruleus (LC) in carotid sinus baroreceptor reflex (CSR) resetting induced by intracerebroventricular (i.c.v.

[Clinical and experimental retrospective analysis on acute leukemia with trisomy 4 cell].

Objective: To explore the clinical and experimental features of acute leukemia (AL) with trisomy 4.

Methods: A retrospective analysis on the clinical and laboratory data of 21 cases of AL with trisomy 4 was performed. Chromosomes were prepared using direct method and/or short-term (24 h) cultures of bone marrow cells.

[Clinical and experimental study of 7 cases of acute lymphoblastic leukemia with dic(7;9) (pll;pll)].

Objective: To investigate the laboratory and clinical features of 7 cases of acute lymphoblastic leukemia (ALL) with dic(7;9) (pll;pll).

Methods: Cytogenetic examination of bone marrow cells was performed by direct method or short-term culture method. R banding technique was used for karyotype analysis.