Nanozyme based colorimetric detection of biogenic gaseous HS using Ag@Au core/shell nanoplates with peroxidase-like activity.

A highly sensitive and facile colorimetric assay is introduced for detecting biogenic gaseous HS using peroxidase (POD)-like catalytic activity of silver core/gold shell nanoplates (Ag@Au NPls). HS can react with Ag@Au NPls to form AgS or AuS on their surface, which can reduce POD-like activity of Ag@Au NPls and consequently decrease the absorbance at 650 nm due to oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (HO). For in situ and multiple detection of HS, we utilized a microplate cover with 24 polydimethylsiloxane inner wells where Ag@Au NPls reacted with HS gas followed by treatment with TMB/HO.

Simple and Sensitive Detection of Bacterial Hydrogen Sulfide Production Using a Paper-Based Colorimetric Assay.

Hydrogen sulfide (HS) is known to participate in bacteria-induced inflammatory response in periodontal diseases. Therefore, it is necessary to quantify HS produced by oral bacteria for diagnosis and treatment of oral diseases including halitosis and periodontal disease. In this study, we introduce a paper-based colorimetric assay for detecting bacterial HS utilizing silver/Nafion/polyvinylpyrrolidone membrane and a 96-well microplate.

Cytotoxicity evaluation of sodium lauryl sulfate in a paper-based 3D cell culture system.

Because three-dimensional (3D) cell culture is more similar to cell microenvironments than two-dimensional (2D) cell culture, various 3D cell culture systems have been developed. Recently, paper has been used as a promising material for 3D cell culture and tissue models due to its flexibility, ease of manufacture, low cost, and widespread accessibility. In this study, we fabricated a paper-based 3D cell culture platform consisting of a hydrophilic region for cell attachment and a hydrophobic region printed with wax.

Rapid and simple colorimetric detection of hydrogen sulfide using an etching-resistant effect on silver nanoprisms.

A fast and sensitive colorimetric paper sensor has been developed using silver nanoprisms (Ag NPRs) with an edge length of ~50 nm for the detection of free HS gas. We prepared two types of Ag NPRs-coated HS sensing papers: a multi-zone patterned paper for passive (diffusion mode), and a single-zone patterned paper for pumped mode of HS gas. The change in color intensity was quantitatively analyzed of Ag NPRs-coated paper after KCl treatment depending on the concentration of HS gas, from yellow to purplish brown.

Targeting hydrogen sulphide signaling in breast cancer.

Introduction: Hydrogen sulphide (HS) has been established as a key member of the gasotransmitters family that recently showed a pivotal role in various pathological conditions including cancer.

Objectives: This study investigated the role of HS in breast cancer (BC) pathogenesis, on BC immune recognition capacity and the consequence of targeting HS using non-coding RNAs.

Methods: Eighty BC patients have been recruited for the study.

Characterization of H2S releasing properties of various H2S donors utilizing microplate cover-based colorimetric assay.

In this study, we characterized the potential HS-releasing properties of seven different HS donors, including sodium sulfide (NaS), sodium hydrosulfide (NaHS), diallyl disulfide (DADS), diallyl trisulfide (DATS), sodium thiosulfate (NaSO), morpholin-4-ium 4-methoxyphenyl-morpholino-phosphinodithioate (GYY4137), and Lawesson's reagent, in three assay solutions, phosphate buffered saline (PBS, pH 7.4), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffered saline (HBS, pH 7.6), and cell growth media (GM), utilizing our microplate cover-based colorimetric assay.

Paper-Based Surface-Enhanced Raman Spectroscopy for Diagnosing Prenatal Diseases in Women.

We report the development of a surface-enhanced Raman spectroscopy sensor chip by decorating gold nanoparticles (AuNPs) on ZnO nanorod (ZnO NR) arrays vertically grown on cellulose paper (C). We show that these chips can enhance the Raman signal by 1.25 × 10 with an excellent reproducibility of <6%.

Hydrogen sulfide donor, NaHS, stimulates ANP secretion via the K channel and the NOS/sGC pathway in rat atria.

Hydrogen sulfide (HS) is normally produced from l-cysteine in mammalian tissues and related to the pathogenesis of cardiovascular diseases. The aim of this study is to investigate the effects of HS donor on atrial natriuretic peptide (ANP) secretion and define its mechanism using normal and isoproterenol (ISP)-treated rats. Several HS donors were perfused into isolated beating rat atria, and atrial pressure (AP) and ANP secretion were measured.

A label-free cellulose SERS biosensor chip with improvement of nanoparticle-enhanced LSPR effects for early diagnosis of subarachnoid hemorrhage-induced complications.

It is very difficult to predict some complications after subarachnoid hemorrhage (SAH), despite rapid advances in medical science. Herein, we introduce a label-free cellulose surface-enhanced Raman spectroscopy (SERS) biosensor chip with pH-functionalized, gold nanoparticle (AuNP)-enhanced localized surface plasmon resonance (LSPR) effects for identification of SAH-induced cerebral vasospasm and hydrocephalus caused by cerebrospinal fluid (CSF). The SERS biosensor chip was implemented by the synthesis reaction of the AuNPs, which were charged positively through pH level adjustment, onto a negatively-charged cellulose substrate with ξ = -30.

Colorimetric detection of endogenous hydrogen sulfide production in living cells.

Hydrogen sulfide (HS) has received great attention as a third gaseous signal transmitter, following nitric oxide and carbon monoxide. In particular, HS plays an important role in the regulation of cancer cell biology. Therefore, the detection of endogenous HS concentrations within biological systems can be helpful to understand the role of gasotransmitters in pathophysiology.