A Nonessential Sfp-Type Phosphopantetheinyl Transferase Contributes Significantly to the Pathogenicity of .

4'-Phosphopantetheinyl transferases (PPTases) play important roles in the posttranslational modifications of bacterial carrier proteins, which are involved in various metabolic pathways. Here, we found that and encoded a functional AcpS-type and Sfp-type PPTase, respectively, in GMI1000, and both are capable of modifying AcpP1, AcpP2, AcpP3, and AcpP5 proteins. is located on the megaplasmid, which does not affect strain growth and fatty acid synthesis but significantly contributes to the virulence of and preferentially participates in secondary metabolism.

The FabA-FabB Pathway Is Not Essential for Unsaturated Fatty Acid Synthesis but Modulates Diffusible Signal Factor Synthesis in pv. .

Most bacteria use type II fatty acid synthesis (FAS) systems for synthesizing fatty acids, of which the conserved FabA-FabB pathway is considered to be crucial for unsaturated fatty acid (UFA) synthesis in gram-negative bacteria. pv. , the phytopathogen of black rot disease in crucifers, produces higher quantities of UFAs under low-temperature conditions for increasing membrane fluidity.

HetI-Like Phosphopantetheinyl Transferase Posttranslationally Modifies Acyl Carrier Proteins in spp.

In spp., the biosynthesis of the yellow pigment xanthomonadin and fatty acids originates in the type II polyketide synthase (PKS II) and fatty acid synthase (FAS) pathways, respectively. The acyl carrier protein (ACP) is the central component of PKS II and FAS and requires posttranslational phosphopantetheinylation to initiate these pathways.

Liquiritigenin attenuates isoprenaline‑induced myocardial fibrosis in mice through the TGF‑β1/Smad2 and AKT/ERK signaling pathways.

Myocardial fibrosis is a pathological process characterized by excessive accumulation of extracellular matrix in myocardial interstitial spaces. Myocardial fibrosis is a fundamental process in ventricular remodeling and a primary contributor to the progression of heart failure. Liquiritigenin (LQ) is a flavanone compound with anti‑oxidative, anti‑carcinogenic, anti‑inflammatory and estrogenic properties.

Characterization of 3-Oxacyl-Acyl Carrier Protein Reductase Homolog Genes in PAO1.

Unlabelled: Bacterial 3-oxoacyl-ACP reductase (OAR) catalyzes the 3-oxoacyl-ACP reduction step in the fatty acid synthesis pathway. At least 12 genes in the genome are annotated as OAR-encoding genes. In this study, we characterized the functions of these genes with biochemical and genetic techniques.

miR‑15a‑3p affects the proliferation, migration and apoptosis of lens epithelial cells.

The present study investigated the effect of microRNA (miR)‑15a‑3p on the proliferation, migration and apoptosis of lens epithelial cells and its potential mechanism, in order to further elucidate the pathogenesis of age‑related cataracts (ARCs). The HLE‑B3 human lens epithelial cell line was transfected with miR‑15a‑3p mimic. Expression of the miR‑15a‑3p mimic was measured by fluorescence‑based reverse transcription‑quantitative polymerase chain reaction analysis.

Biological Functions of in Branched-Chain Fatty Acid Synthesis and Diffusible Signal Factor Family Production in .

In bacteria, the metabolism of branched-chain amino acids (BCAAs) is tightly associated with branched-chain fatty acids (BCFAs) synthetic pathways. Although previous studies have reported on BCFAs biosynthesis, more detailed associations between BCAAs metabolism and BCFAs biosynthesis remain to be addressed. In this study, we deleted the gene, which encodes ketol-acid reductoisomerase in the BCAAs synthetic pathway, from the pv.

Xanthomonas campestris FabH is required for branched-chain fatty acid and DSF-family quorum sensing signal biosynthesis.

Xanthomonas campestris pv. campestris (Xcc), a Gram-negative phytopathogenic bacterium, causes black rot disease of cruciferous vegetables. Although Xcc has a complex fatty acid profile comprised of straight-chain fatty acids and branched-chain fatty acids (BCFAs), and encodes a complete set of genes required for fatty acid synthesis, there is still little known about the mechanism of BCFA synthesis.

Mapping of Quantitative Trait Loci for Contents of Macro- and Microelements in Milled Rice (Oryza sativa L.).

Macro- and microelement contents are important traits for nutritional quality in rice. In this study, quantitative trait loci (QTLs) for the contents of seven mineral elements in milled rice were detected using recombinant inbred lines (RILs) of the indica rice cross Zhenshan 97/Milyang 46, followed by the validation and fine mapping of a QTL region on the short arm of chromosome 6. A total of 20 QTLs distributed on chromosomes 1, 3, 5, 6, 10, and 11 were detected in the RIL population.

[Clinical study of surgical treatment of ankylosis of temporomandibular joint].

Objective: To analyze the clinical feature of temporomandibular joint (TMJ) ankylosis and to investigate the advantage and disadvantage of all kind of surgery methods of TMJ ankylosis for supplying references to the treatment of TMJ ankylosis.

Methods: Aetiology, sex, age at time of treatment, clinical features, radiographic findings, surgical treatment was reviewed and analyzed.

Results: Trauma (66.

[The effect of gamma interferon gene transfer on airway inflammation in asthmatic mice].

Objective: To study the effect of airway gamma interferon (IFN-gamma) plasmid gene transfer on airway inflammation in asthmatic mice.

Methods: Forty C57BL/6 mice were randomly divided into four groups: a control group (group A), an asthmatic group (group B), a plasmid group (group C) and an IFN-gamma plasmid group (group D), 10 mice in each group. Except group A, other groups were sensitized with 0.