Anticancer effect of miR-96 inhibitor in bladder cancer cell lines.

The present study aimed to investigate the role of microRNA-96 (miR-96) in the proliferation, invasion and apoptosis of bladder cancer cell lines, and the associated mechanisms. The expression of miR-96 and human ether-à--related (HERG1) potassium channel in the normal uroepithelium SV-HUC-1 cell line, and bladder cancer T24 and 5637 cell lines were examined using reverse transcription-polymerase chain reaction or/and western blotting. Transfection with miR-96 inhibitor or scrambled control (SC) was used to study the biological activities of miR-96 in bladder cancer cell lines.

Effect of Retroperitoneal Lavage with Normal Saline Containing Adrenaline on Carbon Dioxide Absorption in Patients Undergoing Retroperitoneal Laparoscopic Surgery.

Objective: To determine the effect of lavage with adrenaline solution on CO2 absorption during retroperitoneal laparoscopic surgery.

Materials And Methods: Sixty patients scheduled to undergo retroperitoneal laparoscopic surgery were divided into an AD group (lavage with normal saline containing adrenaline [1:500,000], n = 30) and an NS group (lavage with normal saline only, n = 30). After the establishment of artificial pneumoperitoneum and before the start of the operation, the retroperitoneal space was irrigated with 300 mL of normal saline with or without adrenaline, depending on the group.

Experimental study of therapy of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel for the treatment of stress urinary incontinence.

Objective: To explore the myoblast formation around the urethra and increase in urethral resistance of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel injection therapy and effect on LPP in SUI rat model.

Methods: Isolation, cultivation, and identification of SD rat bone marrow mesenchymal stem cell were performed. 5-Azacytidine was introduced to induce muscle-like cells.

[Study of fluorescence spectra of blattela germanica allergen, derenatured and renatured Bla g 2].

Recombinant proteins extracted from inclusion body remain in denaturation status. Renaturation in vitro after initial purification is a key step of downstream processing. A common method of renaturation of recombinant proteins is the dilution method.

[Cloning, expression, purification and identification of Der f6 gene and its immunological characteristics from the dust house mite].

Objective: To construct, purify and characterize a recombinant expression plasmid containing Der f6 gene of Dermatophagoides farinae.

Methods: A pair of primers was designed according to the known sequence of Der f6 gene. The live mites identified and cultured locally were picked and the total RNA was extracted.

Establishing an animal model of unstable atherosclerotic plaques.

Background: Atherosclerotic plaque rupture and coronary thrombosis are the main causes of acute coronary syndromes. However, there is no animal model of unstable atherosclerotic plaques. The presence of the p53 gene in advanced atherosclerotic plaques and the sensitivity to p53-induced apoptosis of smooth muscle cells isolated from these plaques prompted us to build an animal model of unstable atherosclerotic plaques using p53 gene transfer.

[Induction of atherosclerotic plaque instability in rabbits after transfection of human wild-type p53 gene].

Objective: To develop an animal model of unstable atherosclerotic plaques.

Methods: Sixty-four New Zealand white rabbits were randomly divided into two groups: group A (n = 54, undergoing balloon-induced abdominal aortic wall injury and then fed on a diet of 1% cholesterol) and group B (n = 10, fed on a diet of 1% cholesterol only). At the end of the eighth week, the rabbits in group A were randomly divided into two subgroups: group A1 (n = 27) and group A2 (n = 27).