mA demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3.

The clinical application of anthracyclines such as doxorubicin (DOX) is limited due to their cardiotoxicity. N6-methyladenosine (mA) plays an essential role in numerous biological processes. However, the roles of mA and mA demethylase ALKBH5 in DOX-induced cardiotoxicity (DIC) remain unclear.

Quantitative Kinetics of the Reaction between CHOO and HO in the Atmosphere.

Criegee intermediates (CIs) are generated from the ozonolysis of unsaturated hydrocarbons in the atmosphere. They have an important role in determining the implications of atmospheric bimolecular reactions with other atmospheric species. The reaction between CHOO and HO plays a crucial role in understanding how CIs impact the HO budget in the atmosphere.

[Analysis of the phase error non-linearity of FTS and discussion about Mertz method].

Fourier transform spectrometer is an important instrument in the remote sensing applications. There are phase error problems in the Fourier transform spectrometer signal processing procedure. In the present paper, the cause of phase error of Fourier transform spectrometers is shown firstly.

Rapid chip-based capillary electrophoretic mobility shift assay with negative pressure injection for the binding study of transcription factor Abf1 in Saccharomyces cerevisiae.

The study on the interactions of nucleic acids with transcription factor (TF) is critical to understand the gene expression at the molecular level. In the present study, a rapid chip-based capillary electrophoretic mobility shift assay with LIF detection has been developed on a PDMS-quartz chip. We used a simple negative pressure injection procedure to avoid the bias of electrokinetic injection and to allow loading of the high salt buffered sample.

Broad profiling of DNA-binding transcription factor activities improves regulatory network construction in adult mouse tissues.

Molecular systematics involves the description of the regulatory networks formed by the interconnections between active transcription factors and their target expressed genes. Here, we have determined the activities of 200 different transcription factors in six mouse tissues using an advanced mouse oligonucleotide array-based transcription factor assay (MOUSE OATFA). The transcription factor signatures from MOUSE OATFA were combined with public mRNA expression profiles to construct experimental transcriptional regulatory networks in each tissue.

Novel high-throughput profiling of human transcription factors and its use for systematic pathway mapping.

Transcription factors (TFs) are crucial components of regulatory networks that control gene transcription. Current TF assays are limited to the analysis of a single TF or require TF-specific antibodies. Here we report the Single Primer Amplification assisted Oligonucleotide Array-based Transcription Factor Assay (SPA-OATFA) which can directly analyze the binding activities of 240 human TFs simultaneously.

Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).

Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data.