Hydrogen attenuates ischaemia-reperfusion injury in skeletal muscles post-limb replantation by activating the NRF2/HO-1 signalling pathway to reduce BAX expression.

Background: Ischaemia-reperfusion injury (IRI) is a critical complication post-limb replantation. The oxidative stress and cellular apoptosis due to IRI considerably hinder the healing process. This study aimed to investigate the modulatory effects of pre-perfusion with hydrogen-rich heparin sodium on the nuclear factor erythroid 2-related factor 2 (NRF2)/haeme oxygenase-1 (HO-1) pathway and its potential mechanisms in mitigating skeletal muscle IRI post-limb replantation.

Multiphysical field and multiobjective mathematical modeling of grain-oilseed storage: Current status and future trends.

Storage is an important process involved in the postharvest treatment of grain-oilseed and is necessary for maintaining high quality and ensuring the long-term supply of these commodities in the food industry. Proper storage practices help prevent spoilage, maintain nutritional value, and preserve marketable quality. It is of great interest for storage to investigate flow, heat and mass transfer processes, and quality change for optimizing the operation parameters and ensuring the quality of grain-oilseed.

[Effects of water and nitrogen management on photosynthetic matter production and yield of wheat].

The shortage of water resources and the irrational application of nitrogen fertilizer restrict the synergistic enhancement of yield and water- and fertilizer-use efficiencies of wheat in the Huang-Huai-Hai region. In this study, we conducted an experiment following two-factor split zone design with three irrigation levels and four nitrogen application rates. The relative water content of the 0-40 cm soil layer was supplemented to 65% (W), 75% (W), and 85% (W) of field water capacity at the jointing and anthesis stages of wheat.

[Effects of phosphorus application rate on distribution of C assimilates and spike formation in different til-lers of wheat with supplementary irrigation].

To clarify the appropriate rate of phosphorus application and physiological mechanism for promoting wheat tillering and efficient utilization of phosphorus fertilizer with supplementary irrigation, we used 'Jimai 22' wheat variety as the test material, to set up three phosphorus application treatments, including low (90 kg PO·hm, P), medium (135 kg PO·hm, P), and high (180 kg PO·hm, P) application rates, with no phosphorus application as the control (P). We increased the relative soil water content of each treatment at join-ting stage and anthesis stage to 70%, and measured the area of tiller node, the content of endogenous hormones, the number of tillers in each tiller position, photosynthetic parameters, the distribution of C assimilates in each stem and tiller, as well as the grain yield and partial productivity of phosphate fertilizer. The results showed that compared with P and P treatments, P significantly increased the area of tiller node and the trans-zeatin (tZ), the photosynthetic parameters of the uppermost expanded leaves of the main stem, the total tillers per plant, and the distribution of C assimilates in each tiller.

[Effects of N, P, and K application rates on distribution of C assimilates, starch accumulation in grains and fertilizer utilization of wheat].

Clarifying the appropriate application rates of N, P, and K fertilizers and the physiological mechanisms of wheat under water-saving recharge irrigation in the North China Plain would provide a theoretical basis for formulating reasonable fertilization plans for high-yield and high-efficiency wheat production. We established four treatments with different amounts of nitrogen (N), phosphorus (PO), and potassium (KO) application: 0, 0, and 0 kg·hm (F), 180, 75, and 60 kg·hm (F), 225, 120, and 105 kg·hm (F), and 270, 165, and 150 kg·hm (F). During the jointing and anthesis stages of wheat, the relative water content of each treatment in the 0-40 cm soil layer was replenished to 70%, to investigate the differences in wheat flag leaf photosynthetic characteristics, distribution of C assimilates, grain starch accumulation, and fertilizer utilization.

[Effects of long-term positioning tillage onC assimilate distribution and grain yield formation in wheat].

To provide a theoretical basis and technical support for the high-yield and high-efficiency production of wheat, we examined the effects of different tillage patterns on wheat grain yield of Jimai 22 and the physiological mechanisms in an experiment with three treatments: 14 years in rotary tillage (R), minimal and no tillage (S), and minimal and no tillage with a 2-year subsoiling interval (SS). We assessed the light interception by wheat plant canopy, the distribution of photosynthate transport, and grain yield for the three cultivation modes. The results showed that leaf area index was significantly higher for SS treatment than the other treatments at 14-28 days after anthesis.

[Inhibition of Autophagy Augments the Anticancer Activity of KPT-330 in Mantle Cell Lymphoma Cells].

Objective: To investigate the influence of novel CRM1 inhibitor KPT-330 on the autophagy of mantle cell lymphoma (MCL) cells, and effect of KPT-330 on the prolifiration of MCL cells in the presence or absence of autophagy inhibitor.

Methods: CCK-8 assay was used to detect the effect of KPT-330 on MCL cell lines Z-138， Jeko-1， Granta-519， Rec-1. The effect of KPT-330 on autophagy features were determined by detecting acidic vesicular organelles (AVO) by MDC staining under fluorescence microscope and detecting protein expression of LC3B-II assessed by Western blot.

Effects of nitrogen application rate on the accumulation of C assimilates after flowering and water-nitrogen use efficiency of wheat under supplemental irrigation based on soil moisture.

This study aimed to explore nitrogen fertilizer management measures to synergistically improve wheat yields and water and nitrogen use efficiency under supplemental irrigation based on soil moisture in the Huang Huai winter wheat area. Wheat variety "Yannong 1212" was used as the test material. There were three nitrogen application levels, 150 kg·hm (N), 210 kg·hm (N), and 270 kg·hm (the conventional nitrogen application rate in the Huang Huai winter wheat area, N), with the relative soil water content of 0-40 cm of each treatment was supplemented to 70% at the jointing and flowering stages.

[Value of intestinal regional oxygen saturation and C-reactive protein in the diagnosis of necrotizing enterocolitis in preterm infants].

Objectives: To study the clinical value of intestinal regional oxygen saturation (rSO) and C-reactive protein (CRP) in the diagnosis of necrotizing enterocolitis (NEC) in preterm infants.

Methods: A prospective observational study was conducted among the preterm infants who were hospitalized in Children's Hospital Affiliated to Anhui Medical University, from October 2020 to December 2021, with 22 infants in the NEC group and 35 infants in the non-NEC group. Intestinal rSO was monitored 24 hours after a confirmed diagnosis of NEC in the NEC group, and serum CRP levels were measured before anti-infection therapy.

Inhibitors Targeting Multiple Janus Kinases From Mediate Inhibition and Apoptosis Against Gastric Cancer Cells the Estrogen Pathway.

Janus kinases (JAKs) play a key role in subtly regulating proliferation, apoptosis, and differentiation of cancer cells, and their inhibitors are actively sought as new drug leads. By developing JAKs based affinity ultrafiltration method coupled with LC/Q-TOF-MS in order to discover selective JAKs inhibitors from total quaternary alkaloids (QAs) from , peak 19 (Berberine) and peak 21 (Chelerythrine) were revealed to exhibit notable selectivity on JAK1, JAK2, and JAK3 over Tyk2. In addition, Chelerythrine showed stronger inhibitory activity than the positive control (Cerdulatinib) on gastric cancer cells (AGS), while Berberine, with weaker inhibition.

Small activating RNA activation of ATOH1 promotes regeneration of human inner ear hair cells.

The loss of inner ear hair cells leads to irreversible acoustic injury in mammals, and regeneration of inner ear hair cells to restore hearing loss is challenging. is a key gene in the development and regeneration of hair cells. Small activating RNAs (saRNAs) can target a gene to specifically upregulate its expression.

Predictors of anatomical and functional outcomes following tympanoplasty: A retrospective study of 413 procedures.

Objectives: To identify the predictors of anatomical and functional outcomes following tympanoplasty.

Study Design: A retrospective cohort study.

Methods: Patients with chronic suppurative otitis media (CSOM) who underwent a tympanoplasty at Peking Union Medical College Hospital from January 1, 2015 to December 31, 2019 were retrospectively included.

Stimulation of ROS Generation by Extract of Leading to G/G Cell Cycle Arrest and Antiproliferation in A549 Cells.

Sprague (WU) is a traditional medicinal plant used for the treatment of various diseases, including cancer, in Africa. This study aimed to evaluate the anti-non-small cell lung cancer (NSCLC) activities of WU against A549 cells and to reveal potential molecular mechanisms. The cytotoxicity of various WU extracts was evaluated with HeLa (cervical cancer), HepG2 (liver cancer), HT-29 (colorectal cancer), and A549 (non-small cell lung cancer) cells by means of Sulforhodamine B (SRB) assay.

Acyl Quinic Acid Derivatives Screened Out from by SOD-Affinity Ultrafiltration LC-MS and Their Antioxidative and Hepatoprotective Activities.

Linn. has been utilized both in the food industry and as a traditional medicine for various ailments, while the responsible chemical components and action mechanisms of its antioxidative and hepatoprotective activities remain unclear. In this work, at least 17 quinic acid derivatives as potential ligands for the superoxide dismutase (SOD) enzyme from   L.

Targeted deletion of Atoh8 results in severe hearing loss in mice.

Atoh8, also named Math6, is a bHLH gene reported to have important functions in the developing nervous system, pancreas and kidney. However, the expression pattern and function of Atoh8 in the inner ear are still unclear. To study the function of Atoh8 in the developing mouse inner ear, we performed targeted deletion of Atoh8 by intercrossing Atoh8 mice.

Identification of Anti-Inflammatory and Anti-Proliferative Neolignanamides from Employing Multi-Target Affinity Ultrafiltration and LC-MS.

Previous reports have illustrated that the incidence and mortality of cancer are increasing year by year worldwide. In addition, the occurrence, development, recurrence and metastasis of cancer are closely related to inflammation, which is a kind of defensive response of human body to various stimuli. As an important medicinal plant in Africa, has been reported to have certain anti-inflammatory and anti-proliferative activities, but its specific components and mechanisms of action remain elusive.

New Lignanamides with Antioxidant and Anti-Inflammatory Activities Screened Out and Identified from Combining Affinity Ultrafiltration LC-MS with SOD and XOD Enzymes.

, also known as "green heart," is widely used for the treatment of various diseases as a traditional ethnomedicinal plant in local communities in Africa. In this work, 9 and 12 potential superoxide dismutase (SOD) and xanthine oxidase (XOD) ligands from were quickly screened out by combining SOD and XOD affinity ultrafiltration with LC-MS, respectively. In this way, four new lignanamides (compounds -) and one new macrocyclic glycoside (compound ), along with three known compounds (compounds , , and ), were isolated and identified firstly in this species.

Comparison of the gut microbiota of short-term and long-term medical workers and non-medical controls: a cross-sectional analysis.

Objectives: The hospital environment has been implicated in the enrichment and exchange of pathogens and antibiotic resistance, but its potential in shaping the symbiotic microbial community of hospital staff is unclear. This study was designed to evaluate the alteration of the gut microbiome in medical workers compared to non-medical controls.

Methods: A prospective cross-sectional cohort study was conducted in the intensive care unit (ICU) and other departments of a centre in north-eastern China.

In Vitro Antibacterial and Antiproliferative Potential of Mattf. (Asteraceae) and Identification of Potential Bioactive Compounds.

Many species belonging to the genus are widely used in traditional medicine to treat infectious diseases and cancers. The present study aimed to evaluate the antibacterial and antiproliferative properties of Mattf. (Asteraceae).

Transmission of COVID-19 in the terminal stages of the incubation period: A familial cluster.

We report a familial cluster of 2019 novel coronavirus disease (COVID-19) to assess its potential transmission during the incubation period. The first patient in this familial cluster was identified during the presymptomatic period, as a close contact of a confirmed patient. Five family members had close contact with this first patient during his incubation period, with four of them confirmed positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in the subsequent sampling tests.

Single-port laparoscopy-assisted vaginal repair of a cesarean scar defect: a single-center retrospective study.

Background: The incidence of uterine cesarean scar defect (niche) is high, and some patients require surgery. Single-port laparoscopy can reduce post-operative pain, and provide better cosmetic effects. This study was performed to evaluate the safety and superiority of single-port laparoscopy-assisted vaginal repair of uterine cesarean scar defect (niche) in women after cesarean section.

[Light energy utilization and distribution characteristics of flag leaf C assimilate in different spike-type wheat varieties and their responses to supplementary irrigation].

To clarify the differences in light energy utilization and distribution characteristics of flag leaf C assimilate in different spike-type wheat varieties and their responses to supplementary irrigation, we set three water treatments in a field experiment, including no irrigation during growth duration of wheat (W), water-saving irrigation (W, irrigating at jointing and anthesis of wheat to keep the relative moisture of 0-40 cm soil to 65% and 70%), full irrigation (W, irrigating at jointing and anthesis of wheat to keep the relative moisture of 0-40 cm soil to 85% and 90%) with the medium-spike wheat cultivars Jimai 22 and Qingnong 2, large-spike wheat cultivars Shannong 23 and Shannong 30 as test materials. The effects of different water treatments on canopy light energy utilization and C assimilate distribution characteristics of two spike-type wheat varieties were examined. The results showed that leaf area index, canopy light interception rate, and light energy utilization rate of the two varieties at 2, 11, 20 and 31 days after anthesis were significantly higher than W treatment, but there was no significant change in each index when the irrigation increased to W treatment.