Clinical and pathological characteristics and prognosis of 132 cases of rectal neuroendocrine tumors.

Background: Neuroendocrine tumors (NETs) frequently occur in the gastrointestinal tract, lung, and pancreas, and the rectum and appendix are the sites with the highest incidence. Epidemiology statistics show that an estimated 8000 people every year in the United States are diagnosed with NETs occurring in the gastrointestinal tract, including the stomach, intestine, appendix, colon, and rectum. The pathological changes and clinical symptoms of NETs are not specific, and therefore they are frequently misdiagnosed.

High-resolution colonic manometry and its clinical application in patients with colonic dysmotility: A review.

The detailed process and mechanism of colonic motility are still unclear, and colonic motility disorders are associated with numerous clinical diseases. Colonic manometry is considered to the most direct means of evaluating colonic peristalsis. Colonic manometry has been studied for more than 30 years; however, the long duration of the examination, high risk of catheterization, huge amount of real-time data, strict catheter sterilization, and high cost of disposable equipment restrict its wide application in clinical practice.

Naso-intestinal tube is more effective in treating postoperative ileus than naso-gastric tube in elderly colorectal cancer patients.

Objective: The objective of this study was to compare the effect of naso-intestinal tube and naso-gastric tube in relieving postoperative ileus in elderly patients with colorectal cancer.

Methods: Patients (n = 46) with ileus symptom following radical surgery for treating colorectal cancer were placed with either naso-intestinal tube at duodenum or conventional naso-gastric tube. Then, their waist perimeter, intra abdominal pressure, maximum diameter in bowls, length of time to pass flatus or passage of bowel movement or to return to diet, length of hospital stay, daily drainage, serum levels of lactic acid, hemoglobin, and creatinine as well were compared.

Construction of Antigenized Antibodies Expressing an Immunodominant Epitope AA32-45 of the Hepatitis C Virus Core Protein.

By using site-directed mutagenesis, we created a unique Xho I site in the CDR3 of the heavy-chain variable domain (V(H)). Two antibody molecules, one carrying one or and the other two repeats of an immunodominant epitope AA32-45 (GVYLLPRRGPRLGV) of the hepatitis C virus core protein in CDR3 of V(H) were engineered and designated Ig-E1, Ig-E2 respectively. We found that both antigenized antibodies lost the HBsAg-binding ability and the insertion of one repeat of GVYLLPRRGPRLGV epitope into the CDR3 of the V(H) domain did not appreciably affect the H chain to assemble with L chain to form a stable H(2)L(2) tetramer.

Separate Expression and Characterization of the Heavy- and the Light-chain of A HBsAg Specific Murine-human Chimeric Antibody in Insect Cells.

Both the heavy- and light-chain of a murine-human chimeric antibody with specificity for hepatitis B virus surface antigen have been expressed separately in a baculovirus expression system. The heavy- and light-chain variable region (VH and VL) genes encoding the marine-monoclonal antibody OH3 were combined with human gamma3 and kappa constant region genes to construct the murine-human chimeric genes, respectively. The transfer vectors containing the chimeric genes were cotransfected into Sf9 cells with linearized virus DNA and recombinant viruses were isolated by dot hybridization, PCR and Southern blot analysis.

High Expression in Insect Cells of A Functional Chimeric Antibody with Specificity for HBsAg.

We have constructed a double-recombinant virus BacHL4.2 containing both the chimeric heavy- and light-chain cDNAs from monoclonal antibody of the HBsAg gene. Both murine-human chimeric antibody heavy- and light-chain were expressed in Sf9 cells infected with a double-recombinant virus BacHL4.

The Cloning and Construction of a Murine-human Chimeric Antibody with Specificity for HBsAg and the Expression of the Chimeric Heavy Chain in E. coli.

The genes encoding the heavy and light chain variable regions (V(H) and V(K)) of the murine monoclonal antibody with the specificity for hepatitis B virus surface antigen (HBsAg) have been clone by using RT-PCR. They were combined to the Human C(gamma)(3) and C(K) genes respectively to construct the murine human chimeric antibody genes The chimeric heavy chain was expressed in E. coli, and characterized by SDS-PAGE, Western-blot, indirect ELISA Assay to demonstrate the expression product has the HBsAg-binding ability.