A length-band fluorescence-based paper analytical device for detecting dipicolinic acid ofloxacin complexation with Cu.

Dipicolinic acid (DPA) is a key biomarker of bacterial spores. In this study, we present a novel distance-based paper analytical device (d-PAD) for the fluorescence sensing of DPA. The detection mechanism relies on the complexation of ofloxacin (OFL) with Cu ions, where Cu quenches the fluorescence of OFL static quenching.

Highly sensitive plasmonic paper substrate fabricated via amphiphilic polymer self-assembly in microdroplet for detection of emerging pharmaceutical pollutants.

We report an innovative and facile approach to fabricating an ultrasensitive plasmonic paper substrate for surface-enhanced Raman spectroscopy (SERS). The approach exploits the self-assembling capability of poly(styrene-b-2-vinyl pyridine) block copolymers to form a thin film at the air-liquid interface within the single microdroplet scale for the first time and the subsequent in situ growth of silver nanoparticles (AgNPs). The concentration of the block copolymer was found to play an essential role in stabilizing the droplets during the mass transfer phase and formation of silver nanoparticles, thus influencing the SERS signals.

Smartphone-based paper strip assay for putrescine and spermidine detection using hybrid organic-inorganic perovskite with Eu complex.

A new method utilizing fluorescent ratiometry is proposed for detecting putrescine and spermidine. The method involves the use of a fluorescent probe comprising a 2D halide perovskite synthesized from octadecylamine-iodine and PbI a grinding-sonicating technique, along with a Eu-complex. Upon excitation at 290 nm, the probe fluoresces at two distinguishable wavelengths.

Triplex glycan quantification by metabolic labeling with isotopically labeled glucose in yeast.

Mass spectrometry-based approaches encompass a powerful collection of tools for the analysis biological molecules, including glycans and glycoconjugates. Unlike most traditional bioanalytical methods focusing on these molecules, mass spectrometry is especially suited for multiplexing, by utilizing stable-isotope labeling. Indeed, stable isotope-based multiplexing can be regarded as the gold-standard approach in reducing noise and uncertainty in quantitative mass spectrometry and quantitative analyses generally.

Novel molecularly imprinted nanogel modified microfluidic paper-based SERS substrate for simultaneous detection of bisphenol A and bisphenol S traces in plastics.

Paper-based surface-enhanced Raman scattering (SERS) optical nanoprobes provide ultrasensitive analyte detection; however, they lack selectivity, making them difficult to use in real-world sample analysis without a pretreatment process. This work describes the design of a microfluidic paper-based SERS substrate based on molecularly imprinted nanogels decorated with silver nanoparticles to simultaneously detect bisphenol A (BPA) and bisphenol S (BPS) traces in plastic toys and receipts. The synthesized nanogels have two characteristics that boost SERS performance: molecularly imprinted cavities that allow for selective adsorption and a wrinkled surface that creates uniformly distributed hot spots.

Silver-based SERS substrates fabricated using a 3D printed microfluidic device.

The detection of harmful chemicals in the environment and for food safety is a crucial requirement. While traditional techniques such as GC-MS and HPLC provide high sensitivity, they are expensive, time-consuming, and require skilled labor. Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical tool for detecting ultralow concentrations of chemical compounds and biomolecules.

Straightforward smartphone assay for quantifying tannic acid in beverages based on colour change of Eu/polyethyleneimine complex.

Tannic acid (TA)-a natural product-is a polyphenol derivative that occurs in certain kinds of beverages. A large amount of TA could give rise to an unpleasant flavour and could negatively affect the human body by causing stomach irritation, abdominal pain, nausea, vomiting, and even death. Thus, the need exists for a simple TA detection procedure that meets specific criteria such as on-site analysis, portability, and affordability.

Turn Off-On Fluorescent CO Gas Detection Based on Amine-Functionalized Imidazole-Based Poly(ionic liquid).

Poly(ionic liquids) (PILs) have been widely used for CO capture because their characteristics resemble those of an ionic liquid, yet they have properties typically associated with polymers. We studied the application of the amine-functionalized poly(vinylimidazole)-based PIL (PVIm-NH) as a chemosensor. The PVIm-NH was successfully prepared by a facile and low-cost method and was characterized by several analytical techniques: proton nuclear magnetic resonance (H NMR), Fourier transform infrared (FT-IR) spectroscopy, gel permeation chromatography (GPC), and spectrofluorometry.

Upconversion Nanomaterials in Bioimaging and Biosensor Applications and Their Biological Response.

In recent decades, upconversion nanomaterials (UCNMs) have attracted considerable research interest because of their unique optical properties, such as large anti-Stokes shifts, sharp emissions, non-photobleaching, and long lifetime. These unique properties make them ideal candidates for unified applications in biomedical fields, including drug delivery, bioimaging, biosensing, and photodynamic therapy for specific cancers. This review describes the general mechanisms of upconversion, synthesis methods, and potential applications in biology and their biological responses.

Self-Assembly of Polystyrene--poly(2-vinylpyridine)/Chloroauric Acid at the Liquid/Liquid Interface.

The self-assembly of polystyrene--poly(2-vinylpyridine) at the liquid/liquid interface has been systematically investigated to develop a series of primary morphologies of the aggregates. The block copolymers self-assembled into large areas of nanodot arrays, parallel nanostrands, layered films, parallel nanobelts, honeycomb monolayers, and foams by reacting with chloroauric acid, depending on the molecular structure of the block copolymers and the amount of chloroauric acid. The formation of the first four ordered structures resulted from interfacial adsorption and self-assembly, and nucleation and epitaxial growth.

Magnetic visible-light activated photocatalyst ZnFeO/BiVO/g-CN for decomposition of antibiotic lomefloxacin: Photocatalytic mechanism, degradation pathway, and toxicity assessment.

Magnetic ZnFeO/BiVO/g-CN (ZBC) composites were prepared via a facile hydrothermal and calcination method for the degradation of a representative antibiotics lomefloxacin (LFX) under visible light irradiation. The optimal photocatalyst ZBC-10 with a ZnFeO:BiVO:g-CN mass ratio of 1:8:10 performed 96.1% removal of LFX after 105 min of illumination.

Water-stable perovskite-loaded nanogels containing antioxidant property for highly sensitive and selective detection of roxithromycin in animal-derived food products.

Luminescent inorganic lead halide perovskite nanoparticles lack stability in aqueous solutions, limiting their application to optical sensors. Here, hybrid CsPbBr-loaded MIP nanogels were developed with enhanced stability in aqueous media. Multifunctional MIP nanogels with antioxidant function and hydrophobic cavities were synthesized from HEMA derivatives in the presence of roxithromycin as a template.

Simple fluorescence optosensing probe for spermine based on ciprofloxacin-Tb3+ complexation.

We developed a facile detection method of spermine based on the fluorescence (FL) quenching of the ciprofloxacin-Tb3+ complex, which shows astrong green emission. Ciprofloxacin (CP) makes efficient bondings to Tb3+ ion as a linker molecule through carboxylic and ketone groups to form a kind of lanthanide coordination polymer. The addition of spermine that competes with Tb3+ ions for the interaction with CP due to its positive charge brings about weakened coordination linkage of CP and Tb3+.

Large-Area Assembly of Metal-Organic Layered Ultrathin Films at the Liquid/Liquid Interface.

Two-dimensional functional metal-organic frameworks and coordination polymers have attracted much attention and have been successfully prepared in solutions and at interfaces through the coordination of ligands to metal ions. However, the preparation of large-area ultrathin ordered films is still a challenge. Here, a modified liquid/liquid interfacial epitaxial growth method has been developed.

Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.

Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions. In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans. In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).

Selective dual detection of Hg and TATP based on amphiphilic conjugated polythiophene-quantum dot hybrid materials.

The design of multifunctional sensors based on biocompatible hybrid materials consisting of conjugated polythiophene-quantum dots for multiple environmental pollutants is a promising strategy for the development of new monitoring technologies. Herein, we present a new approach for the "on-off-on" sensing of Hg and triacetone triperoxide (TATP) based on amphiphilic polythiophene-coated CdTe QDs (PQDs, PLQY ∼78%). The emission of the PQDs is quenched by Hg ions via electron transfer interactions.

Preparation, Properties, and Microbial Impact of Tungsten (VI) Oxide and Zinc (II) Oxide Nanoparticles Enriched Polyethylene Sebacate Nanocomposites.

Nanoparticles of tungsten oxide (WO) and zinc oxide (ZnO) enriched polyethylene sebacate (PES) nanocomposites were prepared through the coprecipitation process and condensation polymerization reaction. The obtained nano-sized particles of WO and ZnO, PES, and nanocomposites (WO-PES NC and ZnO-PES NC) were investigated. The average molecular weight of the cured PES was measured by employing the gel permeation chromatography (GPC) technique.

Recent Advances in Nanomicelles Delivery Systems.

The efficient and selective delivery of therapeutic drugs to the target site remains the main obstacle in the development of new drugs and therapeutic interventions. Up until today, nanomicelles have shown their prospective as nanocarriers for drug delivery owing to their small size, good biocompatibility, and capacity to effectively entrap lipophilic drugs in their core. Nanomicelles are formed via self-assembly in aqueous media of amphiphilic molecules into well-organized supramolecular structures.

Selective optosensing of iron(III) ions in HeLa cells using NaYF:Yb/Tm upconversion nanoparticles coated with polyepinephrine.

Novel polyepinephrine-modified NaYF:Yb,Tm upconversion luminescent nanoparticles (UCNP@PEP) were prepared via the self-polymerization of epinephrine on the surfaces of the UCNPs for selective sensing of Fe inside a cell and for intracellular imaging. The proposed UCNP@PEP probe is a strong blue light emitter (λ = 474 nm) upon exposure to an excitation wavelength of 980 nm. The probe was used for detecting Fe owing to the complexation reaction between UCNP@PEP and Fe, resulting in reduced upconversion luminescence (UCL) intensity.

Surface and morphology analyses, and voltammetry studies for electrochemical determination of cerium(iii) using a graphene nanobud-modified-carbon felt electrode in acidic buffer solution (pH 4.0 ± 0.05).

Trace determination of radioactive waste, especially Ce, by electrochemical methods has rarely been attempted. Ce is (i) a fluorescence quencher, (ii) an antiferromagnet, and (iii) a superconductor, and it has been incorporated into fast scintillators, LED phosphors, and fluorescent lamps. Although Ce has been utilized in many industries due to its specific properties, it causes severe health problems to human beings because of its toxicity.

Solvent-resistant microfluidic paper-based analytical device/spray mass spectrometry for quantitative analysis of C -ceramide biomarker.

We developed a highly efficient and low-cost organic solvents-resistant microfluidic paper-based analytical device (μPAD) coupled with paper spray mass spectrometry (PS-MS) for quantitative determination of C -ceramide as a prognostic biomarker for several diseases. Several models of μPAD patterns have been examined to select the most resistant and efficient microchannel barriers, which can provide continuous spray at ionization zone and prevent "coffee ring" effect. Moreover, the developed μPAD has enabled the analysis of low concentration of C -ceramide because of the maximum supply of deposited analyte through microchannel.

Novel "turn on-off" paper sensor based on nonionic conjugated polythiophene-coated CdTe QDs for efficient visual detection of cholinesterase activity.

An increasing number of patients are living with Alzheimer's disease (AD); thus, the need for a method to detect AD early and sensitively has become urgent, and the demand for an intelligent analytical platform is growing year by year. Abnormal levels of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are known to be indicative of AD. In this work, a novel conjugated polythiophene (CP) compound was successfully combined with CdTe quantum dots (QDs) to improve their selectivity and sensitivity.

Dual emission nonionic molecular imprinting conjugated polythiophenes-based paper devices and their nanofibers for point-of-care biomarkers detection.

Enzyme-based assays have been extensively used for the early diagnosis of disease-related biomarkers. However, these assays are time-consuming, resource-intensive, and infrastructure-dependent, which renders them unsuitable and impractical for use in resource-constrained areas. Thus, there is a strong demand for a biocompatible and potentially generalizable sensor that can rapidly detect cancer biomarkers at ultralow concentration.