Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits.

Objective: To explore the effect of acupotomy intervention on autophagy of chondrocytes in rabbits with knee osteoarthritis (KOA), and to determine the possible mechanisms of acupotomy to alleviate cartilage degeneration.

Methods: The modified Videman method was used to construct a KOA rabbit model. After modeling, 40 rabbits were randomly divided into 4 groups by a random number table: control; KOA (model); KOA + acupotomy (acupotomy), and KOA + sham acupotomy (sham), 10 in each group.

[Effect of acupotomy on mitophagy mediated by PINK1/Parkin pathway in cartilage of rabbits with knee osteoarthritis].

Objective: To observe the effect of acupotomy on mitophagy mediated by PINK1/Parkin pathway in cartilage of rabbits with knee osteoarthritis (KOA), so as to explore its mechanism in inhibiting cartilage damage.

Methods: Twenty-one New Zealand rabbits were randomly divided into normal, model, and acupotomy groups, with 7 rabbits in each group. The KOA rabbit model was established by using the Videman method.

[Effect of needle-knife on chondrocyte apoptosis of knee joint in rabbits with knee osteoarthritis based on CircSERPINE2-miR-1271-5P-ERG axis].

Objective: To observe the effect of needle-knife on the chondrocyte apoptosis of knee joint in rabbits with knee osteoarthritis (KOA) based on the CircSERPINE2-miR-1271-5P-E26 specific transformation-related gene (ERG) axis, and to explore the mechanism of needle-knife for KOA.

Methods: Thirty-six New Zealand white rabbits were randomly divided into a normal group, a model group, a needle-knife group and a sham needle-knife group, 9 rabbits in each group. The rabbits in the model group, the needle-knife group and the sham needle-knife group were treated with modified Videman method to prepare KOA model.

[A 10-year retrospective study of pathogens and antimicrobial resistance in neonatal sepsis].

Objectives: To investigate the changes in the pathogen spectrum and antimicrobial resistance over time in neonatal sepsis.

Methods: The medical data were collected from the neonates who were diagnosed with sepsis in the Second Xiangya Hospital of Central South University from January 2010 to December 2019. The incidence rate of sepsis, the pathogen spectrum, and the characteristics of antimicrobial resistance were analyzed.

[Repair impact of vibration exercise with different frequencies on articular cartilage of rats with early knee osteoarthritis and its JNK/NF-κB, SOX9 mechanisms].

To investigate the repair effect and JNK/NF-κB,SOX9 mechanisms of vibration exercise with different frequencies on articular cartilage in rats with early knee osteoarthritis. Forty-eight adult male SD rats were randomly divided into six groups(=8):model control group(MC),high frequency vibration group 1 (GP,60 Hz),high frequency vibration 2 group (GP,40 Hz),medium frequency vibration group (ZP,20 Hz),minor frequency group(DP,10 Hz)and normal control group(NC). Except for NC group,the rats in each group were made into early knee osteoarthritis model after six weeks of knee joint cavity injection of papain solution and 2% mixture l-cysteine on the 1,4 and 7 day.

[Effect of needle knife on mTOR/Atg/ULK1/Beclin-1 axis and chondrocyte autophagy in rats with knee osteoarthritis].

Objective: To observe the effect of needle knife on chondrocyte autophagy and expressions of autophagy-related protein and mammalian target of rapamycin (mTOR) in rats with knee osteoarthritis (KOA), and to explore the possible mechanism of needle knife for KOA.

Methods: A total of 42 SD rats were randomly divided into a normal group, a model group and a needle knife group, 14 rats in each group. Except for the normal group, the other two groups were injected with the mixture of papain and L-cysteine into the left hind knee joint to establish the KOA model.

Etiology, antimicrobial resistance, and risk factors of neonatal sepsis in China: a systematic review and meta-analysis from data of 30 years.

Objective: To evaluate the regional etiology, antimicrobial resistance (AMR) pattern, and risk factors in neonates with sepsis in China.

Methods: We performed a systematic review and meta-analysis by searching Medline, Embase, Scopus, and Web of Science in December 2020. Studies of neonatal sepsis from China published between 2011 and 2020 were included.

Mechanism of Action of Acupotomy in Inhibiting Chondrocyte Apoptosis in Rabbits with KOA through the PI3K/Akt Signaling Pathway.

Objective: We examined the effects of acupotomy on the PI3K/Akt signaling pathway to elucidate the mechanism of action of acupotomy on articular chondrocyte apoptosis among rabbits with knee osteoarthritis (KOA).

Methods: New Zealand rabbits were randomly assigned to a healthy control group, placebo group, acupotomy group, and drug group, with 10 rabbits in each group. Changes in chondrocytes were examined by hematoxylin and eosin staining, and articular chondrocyte apoptosis was measured by electron microscopy and immunofluorescence.

Role of the PERK-eIF2-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis.

Needle knife therapy, a form of acupuncture and moxibustion, has been widely used in the clinical treatment of knee osteoarthritis (KOA). However, the mechanism is not clear. Therefore, we studied the mechanisms of action of needle knife intervention on KOA in rabbits, with the PERK-eIF2-CHOP pathway as a starting point, in order to determine the mechanism underlying knee joint chondrocyte apoptosis.

[Effects of conservation tillage on soil water condition and winter wheat yield in farmland].

Conservation tillage is one of the most important agricultural management measures on soil water conservation and crop yield increments. Based on long-term experiment during 2011 to 2016, effects of different tillage treatments, including conventional tillage, no-tillage and subsoiling treatments, on soil water condition, crop yield and water use efficiency were analyzed. The results showed that the average and relative soil water conservation rate was 7.

Investigation and control of a Plasmodium falciparum malaria outbreak in Shan Special Region II of Myanmar along the China-Myanmar Border from June to December 2014.

Background: From 2007 to 2013, intensive control measures reduced malaria burden by 90 % along the China-Myanmar border. However, despite these measures a P. falciparum malaria outbreak was reported in the Shan Special Region II of Myanmar in June of 2014.

Genetic polymorphisms of the TNF-α-308G/A are associated with metabolic syndrome in asthmatic patients from Hebei province, China.

Asthma is a complex inflammatory disease involving the critical actions of several important cytokines. Epidemiological data show that obesity could increase the risk of asthma, and insulin resistance, or metabolic syndrome are an important risk factor for obesity asthma. Some studies identified that upstream of the transcription start site within the TNF-α gene promoter region-308 polymorphism was associated insulin resistance or metabolic disorders, while this site was closely related to asthma.

MicroRNA-410 promotes cell proliferation by targeting BRD7 in non-small cell lung cancer.

miR-410 acts as an oncogene or tumor suppressor gene in some malignancies. However, its role in NSCLC is still unknown. In this study, we showed that the expression of miR-410 was up-regulated in both human NSCLC tissues and cells.

[Relationship between serum adiponectin and bone mineral density in preterm infants].

Objective: To examine serum adiponectin level in preterm infants and to evaluate the relationship between serum adiponectin and bone mineral density in preterm infants.

Methods: Seventy-two appropriate-for-gestational-age neonates were classified into three groups according to their gestational ages: early preterm (31-33(+6) weeks, 13 cases), late preterm (34-36(+6) weeks, 16 cases), and full-term (37-42 weeks, 43 cases). Venous blood was collected at one week of their life to measure serum adiponectin concentration.

[Mutational analysis of MYO1E in Chinese children with familial steroid-resistant nephrotic syndrome].

Objective: Steroid-resistant nephrotic syndrome (SRNS) with MYO1E mutations has been identified as autosomal recessive focal segmental glomerulosclerosis (FSGS). To date, only two homozygous mutations in the MYO1E gene were reported in three families with FSGS. This study aimed to examine mutations in the MYO1E gene in children with familial SRNS in the Han Chinese ethnic group.

[First flush effects of storm events of Baoxiang River in Lake Dianchi Watershed].

To understand riverine process of non-point source effectively, first flush effects of storm events were investigated at Baoxiang River of Lake Dianchi Watershed. Three sampling stations were selected along Baoxiang River for observing the flow rate and pollutant concentrations of the first three storm events from June 2009 to August 2009. Net discharged volume, net discharged loading, and net event mean concentration (EMC(n)) were proposed with their calculation methods.

Exogenous hydrogen sulfide protects against traumatic hemorrhagic shock via attenuation of oxidative stress.

Objective: This study was designed to investigate the protective effects of exogenous hydrogen sulfide (H(2)S) on trauma-hemorrhagic shock (T-H).

Materials And Methods: Forty-eight male Sprague-Dawley rats were anesthetized, while 32 were subjected to both midline laparotomy and hemorrhagic shock (35-40 mmHg for 90 min) by bleeding them from the femoral artery. One hour later, resuscitation was initiated with Ringer lactate.

[Effects of ground cover and water-retaining agent on winter wheat growth and precipitation utilization].

An investigation was made at a hilly upland in western Henan Province to understand the effects of water-retaining agent (0, 45, and 60 kg x hm(-2)), straw mulching (3000 and 6000 kg x hm(-2)), and plastic mulching (thickness < 0.005 mm) on winter wheat growth, soil moisture and nutrition conditions, and precipitation use. All the three measures promoted winter wheat growth, enhanced grain yield and precipitation use efficiency, and improved soil moisture and nutritional regimes.

[Responses of winter wheat photosynthetic characteristics and chlorophyll content to water-retaining agent and N fertilizer].

The effects of water-retaining agent (60 kg x hm(-2)) and nitrogen fertilizer (0, 225, and 450 kg x hm(-2)) on the leaf photosynthetic characteristics, chlorophyll content, and water utilization of winter wheat at jointing and grain-filling stages were studied under field conditions. In all treatments, the net photosynthetic rate, stomata conductance, intercellular CO2 concentration, water use efficiency, and chlorophyll content were greater at grain-filling stage than at jointing stage. Under nitrogen fertilization but without water-retaining agent application, the water use efficiency (WUE) of single leaf at jointing stage increased with increasing nitrogen fertilization rate, while the net photosynthetic rate, stomata conductance, intercellular CO2 concentration, and transpiration rate decreased after an initial increase.

[Effects of different application rates of water-retaining agent on root physiological characteristics of winter wheat at its different growth stages].

A field experiment was conducted at the Yuzhou Experimental Base of Henan Province to study the effects of different application rates (0, 30, 60, and 90 kg x hm(-2)) of water-retaining agent (WRA) on the root physiological characteristics, biomass, and grain yield of two winter wheat cultivars Zhengmai-9694 and Aikang-58, aimed to probe into the action mechanisms of WRA on the root system of winter wheat at its different growth stages. The application of WRA decreased the root membrane permeability and soluble sugar content, and increased the root vigor. After the application of WRA, the Zhengmai-9694 at its different growth stages had a greater decrement of root membrane permeability, compared with Aikang-58.

Analysis of spatial and temporal water pollution patterns in Lake Dianchi using multivariate statistical methods.

Various multivariate statistical methods including cluster analysis (CA), discriminant analysis (DA), factor analysis (FA), and principal component analysis (PCA) were used to explain the spatial and temporal patterns of surface water pollution in Lake Dianchi. The dataset, obtained during the period 2003-2007 from the Kunming Environmental Monitoring Center, consisted of 12 variables surveyed monthly at eight sites. The CA grouped the 12 months into two groups, August-September and the remainder, and divided the lake into two regions based on their different physicochemical properties and pollution levels.

The effect of ketamine on N-methyl-D-aspartate receptor subunit expression in neonatal rats.

Background And Objective: Ketamine has been widely used in paediatric anaesthesia but its influence on development in infants and toddlers still remains unclear. In order to elucidate the influence of ketamine on brain development in neonatal rats, semiquantitative reverse transcriptase PCR, quantitative reverse transcriptase PCR and immunohistochemistry assays were performed to detect the expression of N-methyl-D-aspartate receptor subtypes expression.

Methods: Seven-day-old rats were divided into two random groups.

[Soil infiltration capacity under different vegetations in southern Ningxia Loess hilly region].

A new apparatus for measuring the run off-on-out under simulated rainfall conditions was used to study the soil infiltration capacity under different rainfall intensities and vegetations in loess hilly region of southern Ningxia, with the relationships between soil water-stable aggregate content and soil stable infiltration rate under different vegetations analyzed. The results showed that the regression equations between rainfall duration and soil infiltration rate under different vegetations all followed y = a + be(-cx), with R2 ranged from 0.9678 to 0.