Publications by authors named "Yolande Dalpe"
Rhizophagus irregularis is the model species for arbuscular mycorrhizal fungi (AMF) research and the most widely propagated species for commercial plant biostimulants. Using asymbiotic and symbiotic cultivation systems initiated from single spores, advanced microscopy, Sanger sequencing of the glomalin gene, and PacBio sequencing of the partial 45S rRNA gene, we show that four strains of R. irregularis produce spores of two distinct morphotypes, one corresponding to the morphotype described in the R.
View Article and Find Full Text PDFSoil salinity constitutes a major abiotic stress that contributes to soil degradation and crop yield reduction. Using arbuscular mycorrhizal fungi (AMF) inoculation can help to alleviate these deleterious effects. Most researches on AMF application are dealing with ecological restoration, whereas little consideration has been given to agriculture and legume production.
View Article and Find Full Text PDF(1) Background: Soil degradation is an increasingly important problem in many parts of the world, particularly in arid and semiarid areas. Arbuscular mycorrhizal fungi (AMF) isolated from arid soils are recognized to be better adapted to these edaphoclimatic conditions than exogenous ones. Nevertheless, little is known about the importance of AMF inoculum sources on development in natural saline soils.
View Article and Find Full Text PDFArbuscular mycorrhizal fungi (AMF) are plant root symbionts that continuously carry thousands of nuclei in their spores and hyphae. This unique cellular biology raises fundamental questions regarding their nuclear dynamics. This review aims to address these by synthesizing current knowledge of nuclear content and behavior in these ubiquitous soil fungi.
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- - Arbuscular mycorrhizal fungi (AMF) form symbiotic relationships with most land plants, but coding information is mostly available only for a few well-studied species like Rhizoglomus and Gigaspora.
- - This study presents large-scale transcriptome data from eight lesser-known AMF species, revealing their functional diversity and confirming evolutionary connections among them.
- - The research shows that RNA-seq from low-input RNA is as reliable as traditional methods, paving the way for better understanding of fungal functions and phylogenetic relationships with minimal RNA samples.
Very few studies reported the potential of arbuscular mycorrhizal symbiosis to dissipate hydrocarbons in aged polluted soils. The present work aims to study the efficiency of arbuscular mycorrhizal colonized wheat plants in the dissipation of alkanes and polycyclic aromatic hydrocarbons (PAHs). Our results demonstrated that the inoculation of wheat with Rhizophagus irregularis allowed a better dissipation of PAHs and alkanes after 16 weeks of culture by comparison to non-inoculated condition.
View Article and Find Full Text PDFSoil salinization is an increasingly important problem in many parts of the world, particularly under arid and semi-arid areas. Unfortunately, the knowledge about restoration of salt affected ecosystems using mycorrhizae is limited. The current study aims to investigate the impact of salinity on the microbial richness of the halophytic plant Tamarix articulata rhizosphere.
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- Arbuscular mycorrhizal fungi (AMF) can enhance the ability of the plant Miscanthus × giganteus to remediate pollution in contaminated soils, which is both an ecological and economic solution for soil rehabilitation.
- The study focused on how AMF affects the plant's response to trace elements (TE) by analyzing fatty acid compositions and oxidative stress indicators in both roots and leaves under contaminated vs. control soil conditions.
- Results showed that while roots accumulated more TEs, leaves suffered greater damage, leading to increased oxidative stress, indicating that AMF inoculation can improve plant health and enhance its ability to cope with soil pollution.
Article Synopsis
- * Researchers studied the genetic makeup of three AMF isolates, focusing on genes related to mating (MATA-HMG), and found at least 76 such genes in a specific AMF species, Rhizophagus irregularis.
- * The discovery of a unique gene region in R. irregularis suggests that these fungi may have more genetic diversity and potential for hidden sexual reproduction than previously understood.
The use of microwave irradiation as a source of energy to clear and stain intra-radical arbuscular mycorrhizal fungi propagules has been tested on a variety of indigenous and cultivated herbaceous plants. The aim of the study was to evaluate the efficiency of microwave irradiation on root softening, fungi tissue staining, and preservation of DNA integrity for subsequent molecular analyses. The proposed methodology has been adapted from the standard procedures used to detect and quantify mycorrhizal root colonization levels.
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- In sexual reproduction, offspring inherit half of their genome from each parent, but the inheritance patterns in microorganisms, especially coenocytic organisms like Arbuscular Mycorrhizal Fungi (AMF), can be complex and not fully understood.
- A study using live confocal microscopy showed that the number of nuclei in AMF spores is linked to their size, with these spores capable of passing on hundreds of nuclei through new symbioses, indicating a unique method of reproduction.
- The research concludes that AMF spores contain nuclei from both migration and mitosis, suggesting that AMF may not experience a genetic bottleneck typical of other eukaryotic organisms, raising fascinating questions about their genetic inheritance.
An inorganic phosphate transporter gene sequence (852-bp section) allowed discrimination between 10 Glomus fungal species represented by 25 strains. It was particularly valuable in differentiating between morphologically similar species with nucleotide and amino acid sequence differences higher than 3%. This gene is proposed as a reliable barcode for the Glomeromycetes.
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- Researchers successfully grew a strain of Acaulospora rehmii in vitro using Ri T-DNA transformed carrot roots, allowing for detailed observation of its spore development and hyphal structures.* -
- The study noted that the sporogenous hyphae developed intercalarly and formed spherical structures known as sporiferous saccules, which eventually collapsed after spore maturation.* -
- The findings added new insights into the early developmental stages of Acaulospora, aligning with previous studies on other species while introducing unique characteristics of intercalary hyphal swellings.*
Article Synopsis
- - The City of Montreal studied the environmental impact of in-ground copper-treated baskets on root growth control for Norway maple trees, finding that the release of copper from the baskets decreased significantly over two years, dropping to just 2% of the initial amount applied.
- - Total copper content increased in both the soil around the baskets and the baskets themselves over the study period, but phosphorus application levels did not affect copper release.
- - Inoculation with the symbiotic fungus Glomus intraradices did increase copper metal release but not for copper hydroxide, and it did not effectively enhance root colonization, indicating that the basket conditions were not ideal for the fungus compared to trees grown in open fields.