[Investigation of the variation of Cry j 2 concentration in pollen among sugi (Cryptomeria japonica d. Don) trees using a newly established extraction method].

Background: Selection of low-allergen trees is under consideration for the reduction of Sugi (Cryptomeria japonica) pollinosis. The extraction method of Cry j 2 was established and then the variation of Cry j 2 concentrations in pollen among Sugi trees was investigated.

Methods: The extraction efficiency of Cry j 2 was compared among buffers with different pH, ionic species and NaCl concentration.