Combination Effects of Plant Extracts Rich in Tannins and Saponins as Feed Additives for Mitigating in Vitro Ruminal Methane and Ammonia Formation.

The objective of this experiment was to test the effects of combining plant extracts rich in tannins and saponins at varying proportions on in vitro ruminal methane and ammonia formation. Tannins were extracted from leaves and saponins from fruits with various solvents. The extracts obtained with the most efficient solvents (tannins: 75% water and 25% methanol; saponins: pure methanol) were then used in vitro.