Complete mitochondrial genome of Sebastes taczanowskii (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea.

Sebastes taczanowskii is a subarctic species in the north-western Pacific Ocean. To obtain mitochondrial DNA sequences for phylogeny, the complete mitogenome (16 452 bp in length) of S. taczanowskii was constructed using next-generation sequencing.

Complete mitochondrial genome of Sebastes steindachneri (Scorpaenidae: Scorpaeniformes) from the East Sea, Korea.

Sebastes is a diverse genus of fish in the family Scorpaenidae. The larvae and adults in this genus have similar morphological characteristics. To obtain basic information for their identification, the mitogenome of S.

Complete mitochondrial genome of Sebastes owstoni (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea.

Sebastes owstoni is a popular fishery species in the north-western Pacific Ocean. To obtain basic information on its mitochondrial DNA (mtDNA), the complete mitogenome of S. owstoni was subjected to the next-generation sequencing.

Complete mitochondrial genome of Sebastes longispinis (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea.

Sebastes longispinis is considered to be a valid species despite its incomplete mitochondrial lineage. To obtain basic information of this species, the complete mitochondrial DNA sequence of S. longispinis was determined by next-generation sequencing.

Complete mitochondrial genome of Sebastes hubbsi (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea.

Hybrids of Sebastes hubbsi, considered a valid species, have been found in the ocean. To examine the basic characteristics of mitochondrial DNA, we analysed the complete mitogenome of S. hubbsis using next-generation sequencing.

Complete mitochondrial genome of Sebastes vulpes (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea.

Sebastes vulpes is considered a valid species despite the presence of extensive hybrids. To obtain the basic genetic intraspecific structure, we analyzed the complete mitogenome of S. vulpes using next-generation sequencing.

The complete mitochondrial genome of Sebastes pachycephalus (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea.

Due to variations in coloration as well as other morphological features, the identification of Sebastes pachycephalus has been problematic. The complete mitogenome of S. pachycephalus was acquired using next-generation sequencing.

The complete mitochondrial genome of the oblong rockfish Sebastes oblongus (Scorpaenidae, Scorpaeniformes).

The complete mitogenome of the oblong rockfish Sebastes oblongus was constructed using next-generation sequencing. The full genome was 16,396 bp in length, including two rRNA, 22 tRNA, one control region and 13 protein-coding genes (PCGs). The genome consisted of 28.

The complete mitochondrial genome of the dark-banded rockfish Sebastes inermis (Scorpaenidae, Scorpaeniformes).

The complete mitochondrial genome of the dark-banded rockfish Sebastes inermis was determined using next-generation sequencing, including Sanger sequencing. The full genome was 16,504 bp in length, including 2 rRNA, 22 tRNA and 13 protein-coding genes (PCGs), as well as the control region. The genome was 27.

The complete mitochondrial genome of the Hwanghae Rockfish Sebastes koreanus (Scorpaenidae, Scorpaeniformes).

The complete mitochondrial genome sequence of Sebastes koreanus was determined by next-generation sequencing. The total genome was 16,499 bp in length, comprising two rRNA, 22 tRNA and 13 protein-coding genes (PCGs), as well as the mtDNA control region. The genome constitutes 27.

Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii).

To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate γ-linolenic acid (18:3 n-6) to di-homo-γ-linolenic acid (20:3 n-6).

Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli).

Delta 6-fatty acid desaturase (D6DES) is used in the synthesis of polyunsaturated fatty acids (PUFAs) from microorganisms to higher animals, including arachidonic acid (ARA) and eicosapentaenoic acid (EPA). A 1,338 bp full-length cDNA encoding D6DES was cloned from Acanthopagrus schlegeli (AsD6DES) through degenerate- and RACE-PCR methods. A recombinant vector expressing AsD6DES (pYES-AsD6DES) was subsequently constructed and transformed into Saccharomyces cerevisiae to test the enzymatic activity of AsD6DES towards the production of n-6 and n-3 fatty acids.