Fiber-coupled 2 mL vacuum-gap Fabry-Perot reference cavity for portable laser stabilization.

Vacuum-gap Fabry-Perot cavities are indispensable for the realization of frequency-stable lasers, with applications across a diverse range of scientific and industrial pursuits. However, making these cavity-based laser stabilization systems compact, portable, and rugged enough for use outside of controlled laboratory conditions has proven difficult. Here, we present a fiber-coupled 1396 nm laser stabilization system requiring no free-space optics or alignment, built for a portable strontium optical lattice clock.

A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle.

Muscle fiber properties exert a significant influence on pork quality, with cross-sectional area (CSA) being a crucial parameter closely associated with various meat quality indicators, such as shear force. Effectively identifying and segmenting muscle fibers in a robust manner constitutes a vital initial step in determining CSA. This step is highly intricate and time-consuming, necessitating an accurate and automated analytical approach.

Photonic chip-based low-noise microwave oscillator.

Numerous modern technologies are reliant on the low-phase noise and exquisite timing stability of microwave signals. Substantial progress has been made in the field of microwave photonics, whereby low-noise microwave signals are generated by the down-conversion of ultrastable optical references using a frequency comb. Such systems, however, are constructed with bulk or fibre optics and are difficult to further reduce in size and power consumption.

Automatic Recognition and Quantification Feeding Behaviors of Nursery Pigs Using Improved YOLOV5 and Feeding Functional Area Proposals.

A novel method is proposed based on the improved YOLOV5 and feeding functional area proposals to identify the feeding behaviors of nursery piglets in a complex light and different posture environment. The method consists of three steps: first, the corner coordinates of the feeding functional area were set up by using the shape characteristics of the trough proposals and the ratio of the corner point to the image width and height to separate the irregular feeding area; second, a transformer module model was introduced based on YOLOV5 for highly accurate head detection; and third, the feeding behavior was recognized and counted by calculating the proportion of the head in the located feeding area. The pig head dataset was constructed, including 5040 training sets with 54,670 piglet head boxes, and 1200 test sets, and 25,330 piglet head boxes.

Posture Detection of Individual Pigs Based on Lightweight Convolution Neural Networks and Efficient Channel-Wise Attention.

In this paper, a lightweight channel-wise attention model is proposed for the real-time detection of five representative pig postures: standing, lying on the belly, lying on the side, sitting, and mounting. An optimized compressed block with symmetrical structure is proposed based on model structure and parameter statistics, and the efficient channel attention modules are considered as a channel-wise mechanism to improve the model architecture.The results show that the algorithm's average precision in detecting standing, lying on the belly, lying on the side, sitting, and mounting is 97.

MG53, A Novel Regulator of KChIP2 and I, Plays a Critical Role in Electrophysiological Remodeling in Cardiac Hypertrophy.

Background: KChIP2 (K channel interacting protein) is the auxiliary subunit of the fast transient outward K current ( I) in the heart, and insufficient KChIP2 expression induces I downregulation and arrhythmogenesis in cardiac hypertrophy. Studies have shown muscle-specific mitsugumin 53 (MG53) has promiscuity of function in the context of normal and diseased heart. This study investigates the possible roles of cardiac MG53 in regulation of KChIP2 expression and I, and the arrhythmogenic potential in hypertrophy.

High-throughput label-free molecular fingerprinting flow cytometry.

Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner.

Rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectroscopy with heterodyne detection.

High-speed Raman spectroscopy has become increasingly important for analyzing chemical dynamics in real time. To address the need, rapid-scan Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) spectroscopy has been developed to realize broadband CARS measurements at a scan rate of more than 20,000 scans/s. However, the detection sensitivity of FT-CARS spectroscopy is inherently low due to the limited number of photons detected during each scan.

Decreased KCNE2 Expression Participates in the Development of Cardiac Hypertrophy by Regulation of Calcineurin-NFAT (Nuclear Factor of Activated T Cells) and Mitogen-Activated Protein Kinase Pathways.

Background: KCNE2 is a promiscuous auxiliary subunit of voltage-gated cation channels. A recent work demonstrated that KCNE2 regulates L-type Ca channels. Given the important roles of altered Ca signaling in structural and functional remodeling in diseased hearts, this study investigated whether KCNE2 participates in the development of pathological hypertrophy.

C-reactive protein stimulates RAGE expression in human coronary artery endothelial cells in vitro via ROS generation and ERK/NF-κB activation.

Aim: The receptor for advanced glycation end-products (RAGE) plays an important role in development of atherosclerosis, and C-reactive protein (CRP) has been found to stimulate its expression in endothelial cells. In this study we investigated how CRP regulated the expression of RAGE in human coronary artery endothelial cells (HCAECs).

Methods: HCAECs were treated in vitro with CRP (50 μg/mL) in combination with a variety of inhibitors.