Publications by authors named "Yiyuan Lu"
The proteins encoded by Influenza A virus (IAV) evade the innate immune system through diverse strategies to facilitate their replication. However, the regulatory mechanisms remain not fully understood. In this study, we identified that the H9N2 PB1 protein suppressed the activities of the IFN-β, ISRE, and NF-κB promoters.
View Article and Find Full Text PDFPolystyrene microplastics (PS) and dibutyl phthalate (DBP) are emerging pollutants widely coexisting in agroecosystems. However, the efficacies of PS as carriers for DBP and their interactive mechanisms on crop safety remain scarce. Here, this study investigated the combined exposure effects and the interacting mechanisms of PS laden with DBP on choy sum (Brassica parachinensis L.
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- E3 ubiquitin ligases play a crucial role in regulating antiviral immune responses during viral infections, particularly in response to RNA viruses.
- The study found that ASB3, an E3 ligase, is upregulated when RNA viruses like influenza A virus are present, and its overexpression inhibits type I interferon responses.
- Animals without ASB3 showed lower susceptibility to viral infections, as ASB3 promotes the degradation of MAVS, which is essential for antiviral signaling, highlighting ASB3's role as a negative regulator of immune responses.
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- The E3 ubiquitin ligase ASB3 plays a key role in regulating inflammatory responses and is linked to inflammatory bowel disease (IBD), showing increased levels in IBD lesions and contributing to disease progression.
- Mice with high levels of ASB3 demonstrated resistance to colitis, which was associated with lower levels of proinflammatory factors like IL-1β and TNF-α, whereas this resistance could be reversed by disrupting gut microbiota.
- ASB3 facilitates the polyubiquitination of TRAF6, affecting its stability and promoting inflammation; thus, targeting ASB3 levels in intestinal cells could be a potential strategy for treating colitis.
In maintaining organismal homeostasis, gut immunity plays a crucial role. The coordination between the microbiota and the immune system through bidirectional interactions regulates the impact of microorganisms on the host. Our research focused on understanding the relationships between substantial changes in jejunal intestinal flora and metabolites and intestinal immunity during porcine epidemic diarrhea virus (PEDV) infection in piglets.
View Article and Find Full Text PDFPigs are the most suitable model to study various therapeutic strategies and drugs for human beings, although knowledge about cell type-specific transcriptomes and heterogeneity is poorly available. Through single-cell RNA sequencing and flow cytometry analysis of the types in the jejunum of pigs, we found that innate lymphoid cells (ILCs) existed in the lamina propria lymphocytes (LPLs) of the jejunum. Then, through flow sorting of live/dead-lineage (Lin)-CD45+ cells and single-cell RNA sequencing, we found that ILCs in the porcine jejunum were mainly ILC3s, with a small number of NK cells, ILC1s, and ILC2s.
View Article and Find Full Text PDFAfrican swine fever (ASF) is a devastating infectious disease of pigs caused by the African swine fever virus (ASFV), which poses a great danger to the global pig industry. Many viral proteins can suppress with interferon signaling to evade the host's innate immune responses. Therefore, the development of an effective vaccine against ASFV has been dampened.
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- Zbtb1 is a gene that plays a crucial role in T cell development and lymphocyte production within the mammalian zbtb gene family.
- Research using techniques like Hi-C, ATAC-seq, and RNA-seq on EL4 cells revealed significant alterations in chromatin interaction, compactness of TAD structures, and chromatin accessibility in cells lacking Zbtb1.
- The study identified 7,778 differentially expressed mRNAs and provided essential insights into how Zbtb1 influences T cell functions, laying the groundwork for future investigations into this gene.
Article Synopsis
- * They performed a joint analysis to explore regulatory relationships among various RNA types (including lncRNAs, mRNAs, microRNAs, and circRNAs) and validated the chromatin structure related to potential target genes through experimental techniques like Hi-C and ATAC-seq.
- * The study concluded that the Zbtb1 gene plays a multifaceted regulatory role in EL4 cells, significantly influencing the expression of specific genes like Brcal and Dennd5d, and
MicroRNA and circRNA Expression Analysis in a Zbtb1 Gene Knockout Monoclonal EL4 Cell Line.
Front Cell Infect Microbiol
July 2021
Zinc finger and BTB domain containing 1(Zbtb1) is a transcriptional suppressor protein, and a member of the mammalian Zbtb gene family. Previous studies have shown that Zbtb1 is essential for T-cell development. However, the role of Zbtb1 in T-cell lymphoma is undetermined.
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- - Lactic acid bacteria (LAB), specifically LGG ATCC 53103, were found to enhance the diversity and abundance of intestinal microbiota in BALB/c mice, indicated by improved indices such as OTU and Shannon, while the Simpson index decreased.
- - LGG not only affected gut microbiota but also regulated the immune system by altering the distribution of various T lymphocytes and boosting the production of Th1 and Th2 cytokines, leading to a balanced immune response.
- - The study revealed that LGG treatment increased IL-17 expression and influenced the levels of regulatory T cells (Treg), suggesting a role in maintaining the balance between Th-17 and Treg cells in the immune system.
Supported Ni catalysts on three mesoporous SiO supports (i.e., SBA-15, MCM-41, and HMS) were prepared using a solid-state reaction between Ni(NO) and organic template-occluded mesoporous SiO.
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