Nicking enzyme assisted amplification combined with CRISPR-Cas12a system for one-pot sensitive detection of APE1.

Apurinic/apyrimidinic endonuclease 1 (APE1) is a critical enzyme in the base excision repair (BER) pathway, essential for preserving cellular equilibrium. Variations in APE1 activity within blood or tissues can provide significant insights for clinical cancer screening and disease diagnosis. Consequently, the detection of APE1 activity is critical for clinical diagnostics.

Estimates and projections in the economic impacts of 15 dietary risk factors for 204 countries and territories from 2020 to 2050: a health-augmented macroeconomic modelling study.

Background: Suboptimal diet results in significant health and economic burdens. However, the global economic costs of dietary risks remain unclear.

Objective: This study aimed to estimate the macroeconomic burden of 15 dietary risk factors in 204 countries and territories from 2020-2050.

Low-intensity pulsed ultrasound relieved the diabetic peripheral neuropathy in mice via anti-oxidative stress mechanism.

Diabetic peripheral neuropathy (DPN), as one of the most prevalent complications of diabetes, leads to significant pain and financial burden to patients. Currently, there was no effective treatment for DPN since the glucose control was just a prevention and the drug therapy only relieved the DPN pain. As a non-invasive physical therapy, low-intensity pulsed ultrasound (LIPUS) is utilized in the musculoskeletal and nerve injuries therapy.

One-step sensitive detection of UDG activity based on nicking enzyme-assisted signal amplification coupled with APE1 and triggered reporter.

Background: UDG (Uracil-DNA glycosylase) is a pivotal enzyme in the base excision repair (BER) mechanism, and it is widely distributed across most organisms. Its primary function is to identify and excise uracil bases from DNA, thereby facilitating the repair of DNA and the creation of apurinic/apyrimidinic (AP) sites. Furthermore, abnormal expression or dysregulation of UDG activity has been closely associated with ageing, cancer, and other diseases such as immunodeficiency and lymphoma.

One-stage Surgery for Intracardiac Leiomyomatosis.

Introduction: Intracardiac leiomyomatosis is a rare, histologically benign, but biologically aggressive tumor developed from uterus. This study aimed to summarize our experience with one-stage surgery for intracardiac leiomyomatosis.

Methods: We retrospectively reviewed seven patients who underwent surgical treatment for intracardiac leiomyomatosis between May 2016 and November 2021.

Dextran sodium sulfate-induced colitis-like gut permeability and dysbiosis in honeybees.

The gut microbiota has been linked to the pathophysiology of inflammatory bowel disease (IBD). Current animal models are limited in the generation of germ-free animals to evaluate the roles of gut bacteria in intestinal health. Here, we used the honeybee (Apis mellifera) as a model animal to develop colitis-like gut permeability induced by dextran sodium sulfate (DSS) treatment.

Genome assembly of Hawaiian flower thrips Thrips hawaiiensis (Thysanoptera: Thripidae).

The Hawaiian flower thrips, Thrips hawaiiensis, is a common flower inhabiting pest of various horticultural plant species. It damages flowers and fruits by puncturing. T.

A pentatomomorpha-specific salivary protein activates plant immunity and is critical for insect feeding.

The stinkbug , notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant for its benefit. Here, we show that RpSP1, a salivary protein specific to Pentatomomorpha, triggers plant defense responses in multiple plant species. RpSP1 interacts with and stabilizes a HSP40 family protein GmSPIP1 and is dependent on GmSPIP1 to induce cell death.

An orphan viral genome with unclear evolutionary status sheds light on a distinct lineage of flavi-like viruses infecting plants.

Advancements in high-throughput sequencing and associated bioinformatics methods have significantly expanded the RNA virus repertoire, including novel viruses with highly divergent genomes encoding "orphan" proteins that apparently lack homologous sequences. This absence of homologs in routine sequence similarity search complicates their taxonomic classification and raises a fundamental question: Do these orphan viral genomes represent viruses? In 2022, an orphan viral genome encoding a large polyprotein was identified in alfalfa () and thrips (), and named Snake River alfalfa virus (SRAV). SRAV was initially proposed as an uncommon flavi-like virus identified in a plant host distantly related to family .

Identification of novel xanthine oxidase inhibitory peptides from Takifugu obscurus: Peptidomic analysis, molecular docking, and dynamics simulation.

Hyperuricemia, caused by abnormal purine metabolism, is commonly treated with xanthine oxidase (XOD) inhibitors, uricosuric, and dietary adjustments. Recently, marine-derived bioactive peptides have gained attention as potential functional food ingredients due to their therapeutic potential. Takifugu obscurus, an economically significant offshore fish rich in crude proteins was explored in this study as a source of XOD inhibitory peptides.

Identification and Genome Sequencing of Novel Virulent Strains of pv. Causing Rice Bacterial Blight in Zhejiang, China.

pv. () is the causative agent of rice bacterial blight (RBB), resulting in substantial harvest losses and posing a challenge to maintaining a stable global supply. In this study, strains isolated from Shaoxing, Quzhou, and Taizhou, where RBB occurred most frequently in Zhejiang Province in 2019, were selected as the subjects of research.

Association of flap transfer versus excision with secondary healing on patient satisfaction in nasal mass surgery: A cohort study.

Background: Nasal cutaneous tumors impact appearance and function, affecting the patients' well-being. This study compares the outcomes of second intention healing, where wounds heal naturally, with flap transfer in nasal defect repair after tumor resection to evaluate its viability as an alternative.

Methods: In this multi-center study, 80 patients who underwent second intention healing and 70 patients who received flap transfer after nasal tumor resection between July 2018 and June 2023 were included.

Trends in prevalence and multimorbidity of metabolic, cardiovascular, and chronic kidney diseases among US adults with depression from 2005 to 2020.

Background: Comorbid depression and cardiometabolic diseases are prevalent and increase risk of mortality. However, trends in the prevalence and multimorbidity of cardiometabolic diseases in depression are unclear.

Methods: Data of adults aged ≥20 years with depression from the National Health and Nutrition Examination Survey 2005-2020 were analyzed.

A child with factor V deficiency with a novel F5 gene mutation misdiagnosed as a left iliac fossa abscess: A case report.

Rationale: Congenital factor V deficiency is classified as a rare bleeding disorder that is expressed in an autosomal recessive manner and generally occurs in 1 in a million people. This disorder is accompanied by a variety of clinical manifestations, which can easily lead to misdiagnosis. This is the first report to identify the factor V gene variant c.

Ubiquitination in the T Cell Metabolism-Based Immunotherapy in Diseases.

Metabolism refers to the exchange of matter and energy between the organism and the environment and the self-renewal process of matter and energy in the organism. Metabolic activities in cells provide them with energy and various substrates required for development. Naive T cells differentiate into effector T cells and memory T cells after activation, and this process is accompanied by reprogramming of metabolism-related gene expression.

Global Disease Burden Attributable to High Body Mass Index in Young Adults From 1990 to 2019, With Projections to 2050: A Systematic Analysis for the Global Burden of Disease Study 2019.

Aims: The global historical and projected health impacts of the escalating burden of obesity on young adults, who are particularly susceptible to weight gain during transitional life stages, remain insufficiently understood.

Materials And Methods: Using data from the Global Burden of Disease Study 2019, we analysed the disability-adjusted life-years (DALYs) and deaths attributable to high body mass index (BMI) among young adults aged 20-44 years globally and by age, sex, year, location and disease between 1990 and 2019. Future projections until 2050 were further assessed.

N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice.

T cell expansion has a crucial function in both autoimmune and chronic inflammatory diseases, with cycling T cells contributing to the pathogenesis of autoimmune diseases by causing uncontrolled immune responses and tissue damage. Yet the regulatory mechanisms governing T cell expansion remain incompletely understood. Here we show that the enzyme N-acetyltransferase 10 (NAT10) regulates T cell activation and proliferation upon antigen stimulation.

The diagnostic utility of CT attenuation values in detecting calcification within costal cartilage.

Background: To establish and verify diagnostic criteria for the identification of costal cartilage calcification based on computed tomography (CT) attenuation value.

Methods: 360 chest CT slices of 120 patients were reviewed and annotated retrospectively and receiver operating characteristic curve was used to evaluate the diagnostic ability of CT attenuation value. Another 20 slices containing calcification were randomly selected and annotated by 4 doctors for further validation.

The effect of head orientation on vestibular signal-based modulation of paraspinal muscle activity during walking.

Background: Vestibulospinal reflexes play a role in maintaining the upright posture of the trunk. Head orientation has been shown to modify the vestibulospinal reflexes during standing. This study investigated how vestibular signals affect paraspinal muscle activity during walking, and whether head orientation changes these effects.

The potential of arts therapies in Parkinson's disease rehabilitation: A comprehensive review.

Background And Purpose: Parkinson's disease (PD) causes a decline in motor function, cognitive decline, and impacts the mental health of patients. Due to the high cost and side effects of conventional treatments, the medical community has begun to explore safer and more cost-effective alternative therapies. In this context, arts therapies have gained increasing attention as innovative treatments.

Endogenous nege-like viral elements in arthropod genomes reveal virus-host coevolution and ancient history of two plant virus families.

Unlabelled: Negevirus is a recently proposed taxon of arthropod-infecting virus, which is associated with plant viruses of two families ( and ). Nevertheless, the evolutionary history of negevirus-host and its relationship with plant viruses remain poorly understood. Endogenous nege-like viral elements (ENVEs) are ancient nege-like viral sequences integrated into the arthropod genomes, which can serve as the molecular fossil records of previous viral infection.