DOT corrected fluorescence molecular tomography using targeted contrast agents for small animal tumor imaging.

Purpose: To demonstrate diffuse optical tomography (DOT) corrected fluorescence molecular tomography (FMT) for quantitatively imaging tumor-targeted contrast agents in a 4T1 mouse mammary tumor model.

Procedures: In the first set of experiments, we validated our DOT corrected FMT method using subcutaneously injected 4T1 cells pre-labeled with a near-infrared (NIR) Cy 5.5 dye labeled recombinant amino-terminal fragment (ATF) of the receptor binding domain of urokinase plasminogen activator (uPA), which binds to uPA receptor (uPAR) that is highly expressed in breast cancer tissues.

In vivo monitoring of stem cells in Drosophila pupae using the radiative transfer equation-based fluorescence molecular tomography.

Purpose: The aim of this study is to realize in vivo monitoring of DSRed-expressing cell distribution in Drosophila pupae using fluorescence molecular tomography (FMT).

Procedures: The radiative transfer equation (RTE)-based FMT reconstruction algorithm is implemented using finite element method for mesoscopic- or millimeter-scale imaging. The RTE algorithm is validated using both simulated and phantom experimental data.

An optical reflected device using a molecularly imprinted polymer film sensor.

A novel and highly selective optical sensor with molecularly imprinted polymer (MIP) film was fabricated and investigated. The optical sensor head employing a medium finesse molecularly imprinted polymer film has been fabricated and characterised. A blank polymer and formaldehyde imprinted polymer were using methacrylic acid as the functional monomer and the ethylene glycol dimethacrylate as a crosslinker.

DOT guided fluorescence molecular tomography of arbitrarily shaped objects.

The authors demonstrate an approach for truly quantitative three-dimensional fluorescence molecular tomography (FMT) of arbitrarily shaped objects where the distribution of optical properties is obtained using diffuse optical tomography (DOT). The method is tested and evaluated using phantom geometries mimicking mouse anatomic structures. The experiments were conducted using the authors' optical-fiber-free, multiangle transmission system.

Auto-analysis system for graphite morphology of grey cast iron.

The current method to classify graphite morphology types of grey cast iron is based on traditional subjective observation, and it cannot be used for quantitative analysis. Since microstructures have a great effect on the mechanical properties of grey cast iron and different types have totally different characters, six types of grey cast iron are discussed and an image-processing software subsystem that performs the classification and quantitative analysis automatically based on a kind of composed feature vector and artificial neural network (ANN) is described. There are three kinds of texture features: fractal dimension, roughness and two-dimension autoregression, which are used as an extracted feature input vector of ANN classifier.

Quantitative bioluminescence tomography guided by diffuse optical tomography.

We present for the first time experimental evidence that the quantitative accuracy of bioluminescence tomography (BLT) can be significantly improved by incorporating prior spatial distribution of optical properties of heterogeneous media obtained from diffuse optical tomography (DOT). A series of experiments were conducted using a CCD-based scanning system where millimeter-size bioluminescent targets were embedded in a 3 x 3 x 5 cm optically heterogeneous scattering medium. The results show that the BLT images with the recovered optical property distribution in place are considerably better reconstructed compared to that without such prior information, in terms of the location, size and source strength of the targets.

Diffuse optical tomography guided quantitative fluorescence molecular tomography.

We describe a method that combines fluorescence molecular tomography (FMT) with diffuse optical tomography (DOT), which allows us to study the impact of heterogeneous optical property distribution on FMT, an issue that has not been systemically studied. Both numerical simulations and phantom experiments were performed based on our finite-element reconstruction algorithms. The experiments were conducted using a noncontact optical fiber free, multiangle transmission system.

Biosensor for the determination of sorbitol based on molecularly imprinted electrosynthesized polymers.

Despite the increasing number of applications of biosensors in many fields, the construction of a steady biosensor remains still challenging. The high selectivity and stability of molecularly imprinted polymers for the template molecule make them ideal alternatives as recognition elements for sensors. In this work, the fabrication and characterization of biosensor based on molecularly imprinted electrosynthesized polymers is reported as the first case of imprinting sorbitol.