Immunomodulation of Polysaccharides In Vivo Based on Microbiome and Metabolomics Approaches.

is a medicinal herb that can be added to food to provide therapeutic effects and reduce the burden of medications. Herein, the immunomodulatory effects of polysaccharides (GPs) were verified and illustrated by intervening immunocompromised rats treated with different doses of GPs, which were reflected for adjusting the composition and structure of the intestinal microbiota and altering the metabolic profile. The immunomodulatory effects of GPs were exerted by regulating the intestinal microenvironment.

Early venous filling is associated with unfavorable outcomes in acute ischemic stroke with large vessel occlusion after mechanical thrombectomy: a real-world analysis.

Background: The presence of early venous filling (EVF) post-mechanical thrombectomy (MT) in acute ischemic stroke (AIS) patients has been observed, yet its prognostic value for clinical outcomes remains underexplored. This study aimed to assess the correlation between EVF and poor clinical outcomes in AIS patients who underwent MT.

Materials And Methods: This retrospective analysis included AIS patients with large vessel occlusions treated with MT at the First Affiliated Hospital of Xi'an Jiaotong University from January 2018 to June 2023.

Nomogram-Based Prediction of 3-Month Unfavorable Outcome and Early Neurological Deterioration After Endovascular Thrombectomy in Acute Ischemic Stroke.

Background: Some acute ischemic stroke (AIS) patients due to large-vessel occlusion, who underwent endovascular thrombectomy (EVT), continue to experience unfavorable outcomes. Furthermore, the impact of internal carotid artery (ICA) tortuosity remains uncertain. This study aimed to determine the value of ICA tortuosity and clinical features in predicting 3-month unfavorable outcome and early neurological deterioration (END) after EVT in AIS patients through nomograms.

Responsive hydrogel modulator with self-regulated polyphenol release for accelerating diabetic wound healing precise immunoregulation.

Nonhealing chronic wounds are intractable clinical complications of diabetes and are characterized by high protease activity, severe oxidative stress and sustained inflammatory response. In this case, the development of functional hydrogel dressings to modulate the immune microenvironment is a well-known strategy, where the precise stimuli-responsive and spatiotemporally controlled release of bioactive molecules remains a huge challenge. Herein, we developed responsive hydrogels with self-regulated bioactive molecule release based on the protease activity in diabetic wound sites, to serve as a smart immune microenvironment modulator for accelerating wound healing.

An integrated modeling, verification, and code generation for uncrewed aerial systems: less cost and more efficiency.

Uncrewed Aerial Systems (UASs) are widely implemented in safety-critical fields such as industrial production, military operations, and disaster relief. Due to the diversity and complexity of implementation scenarios, UASs have become increasingly intricate. The challenge of designing and implementing highly reliable UASs while effectively controlling development costs and improving efficiency has been a pressing issue faced by academia and industry.

CMR assessment of epicardial edipose tissue in relation to myocardial inflammation and fibrosis in patients with new-onset atrial arrhythmias after STEMI.

Background: Previous studies have shown that epicardial edipose tissue(EAT) appears to be associated with myocardial inflammation and fibrosis, but this is not clear in patients with new-onset atrial arrhythmias after STEMI. The present study focused on using CMR to assess the association of epicardial fat with myocardial inflammation and fibrosis and its predictive value in patients with new-onset atrial arrhythmias after STEMI.

Methods: This was a single-centre, retrospective study.

Autonomous Self-Healing Magnetoelectric I-Skin from Self-Bonded Deep Eutectic Polymer.

Next-generation ionic skin (i-skin) should be self-healing and self-powered, promoting its development toward lightweight, miniaturization, compact, and portable designs. Previously reported self-powered i-skin mostly either lack the ability to self-repair damaged parts or only have self-healing capabilities some components, falling short of achieving complete device self-healability. In this work, a self-bonding strategy is presented to obtain an all-polymerizable deep eutectic solvent (PDES) magnetoelectric i-skin (MIS) that simultaneously achieves self-powering and full-device autonomous self-healability.

Plasticity of cell death pathways ensures GSDMD activation during Yersinia pseudotuberculosis infection.

Macrophages express pattern recognition and cytokine receptors that mediate proinflammatory signal transduction pathways to combat microbial infection. To retaliate against such responses, pathogenic microorganisms have evolved multiple strategies to impede innate immune signaling. Recent studies demonstrated that YopJ suppression of TAK1 signaling during Yersinia pseudotuberculosis infection promotes the assembly of a RIPK1-dependent death-inducing complex that enables caspase-8 to directly cleave and activate gasdermin D (GSDMD).

Optimising Luteinising Hormone Levels on Trigger Day for Improved Ovarian Response and Pregnancy Outcomes in Gonadotropin-Releasing Hormone Antagonist Protocols: A Retrospective Cohort Study.

Objective: To determine the optimal luteinising hormone (LH) level on the trigger day and its impact on pregnancy outcomes in gonadotropin-releasing hormone (GnRH) antagonist protocols using a data-driven approach.

Design: Retrospective cohort study.

Setting: Third Affiliated Hospital of Guangzhou Medical University.

Visual detection of ciprofloxacin using a multi-emission ratiometric fluorescence sensor based on the molecularly imprinted polymers doped with terbium(III) ions.

Ciprofloxacin (CIP) is a commonly used antibiotic, but its abuse may cause bacterial resistance, posing a high risk to the environment and human health. Herein, based on the molecular imprinting technology, this study proposed a ratiometric fluorescence sensor employing the "post-doping" strategy, which aims to be rapid, selective, and visually easy-to-use for CIP detection to address antibiotic residues and environmental risks. Specifically, by exploiting the "antenna effect" of lanthanide metal ions (Ln), terbium (III) (Tb) chosen as a fluorescence-assisted functional monomer as well as the red emitting CdTe quantum dots (QDs) as the internal reference signal were introduced into multi-emission Tb-CdTe@SiO@MIPs (TbMIPs).

Multi-Modal CLIP-Informed Protein Editing.

Proteins govern most biological functions essential for life, and achieving controllable protein editing has made great advances in probing natural systems, creating therapeutic conjugates, and generating novel protein constructs. Recently, machine learning-assisted protein editing (MLPE) has shown promise in accelerating optimization cycles and reducing experimental workloads. However, current methods struggle with the vast combinatorial space of potential protein edits and cannot explicitly conduct protein editing using biotext instructions, limiting their interactivity with human feedback.

Efficient Selection of the 2,4-Dichlorophenoxyacetic Acid Nanobody Gene from the Phage Library Constructed with Sorted Specific Cells and Expression in Plants to Confer Herbicide Resistance.

Immunomodulation in biotechnological processes requires an adequate level of specific, high-affinity recombinant antibodies expressed in the affected cells. Here, we report a new strategy to obtain a sensitive nanobody against the 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide. Unlike the previous methods that used total peripheral blood lymphocytes, specific peripheral lymphocytes stained with a fluorescein isothiocyanate-labeled 2,4-D coating antigen were isolated via fluorescence-activated cell sorting and used for phage display library construction.

Enhanced adhesive hydrogel for emergency hemostasis by balancing adhesion and cohesion.

Hydrogel adhesives have broad application prospects in biomedical fields such as tissue engineering and emergency rescue. However, due to the high water content and defective network structure, their inherent mechanical strength is low, which seriously hinders their application. In the present work, enhanced adhesive hydrogels could be synthesized by balancing cohesion and adhesion forces.

Nonfluorescent Near-Infrared Surface-Enhanced Resonance Raman Nanoprobes with Ultrahigh Brightness and Synergistic Photothermal Effect.

Near-infrared (NIR) surface-enhanced resonance Raman (SERRS) nanoprobes have found wide applications in biomedicine; however, almost all of these nanoprobes are fluorescent because the resonant Raman dyes used cannot be fully quenched onto the underlying plasmonic nanoparticles. Therefore, suppressing the fluorescence backgrounds in resonant Raman spectroscopy imaging is extremely important. In this work, we use a black hole quencher, IQ1, as a Raman dye to develop absolutely nonfluorescent NIR resonant SERRS NPs.

The effect of vitamin D supplementation on antibiotic use: a meta-analysis based on randomized controlled trials.

Objective: This meta-analysis explores the impact of vitamin D supplementation on antibiotic utilization.

Methods: We systematically searched for relevant randomized controlled trials (RCTs) in PubMed, Web of Science, EMBASE, and Science Direct from inception to April 2024. These trials compared antibiotic use rates between groups receiving vitamin D supplements and placebo.

Magnetic biochar prepared from a spent mushroom substrate as an adsorbent for the analysis of pyrethroids in environmental water samples.

In this study, a spent mushroom substrate (SMS) from Lentinus edodes cultivation was used to prepare biochar (BC) and magnetic biochar (MBC) at high temperatures. The magnetic field strength of MBC was proved via VSM analysis. The results of the nitrogen adsorption‒desorption isotherms of BC and MBC showed that MBC exhibited stronger adsorption, and SEM was performed to compare the microstructures of BC and MBC.

Effectiveness of different pharmacological or non-pharmacological interventions on preventing coughing during extubation: a protocol for a systematic review and network meta-analysis.

Introduction: Coughing during extubation can lead to several postoperative complications, including surgical site bleeding, intracranial hypertension and high intraocular pressure. Currently, various pharmacological and non-pharmacological interventions are employed in clinical practice to reduce coughing during extubation. However, it is unclear which of these interventions has the best preventive effect and the fewest adverse events.

Nonsurgical endodontic retreatment of C-shaped maxillary molars: case reports and review of literature.

The root canal systems of maxillary first molar (MFM) and maxillary second molar (MSM) variations represent a clinical challenge for endodontists, especially the prevalence of fused C-shaped roots. Having a thorough knowledge of root canal configuration is an extremely important point for a successful root canal treatment to avoid missing extra canals. The aim of this article was to present 2 cases of maxillary molar with an unusual C-shaped configuration diagnosed during root canal retreatment/treatment and conduct a literature review of the MFM and MSM anatomy.

Highly Monodisperse Stable Gold Nanorod Powder for Optical Sensor.

Gold nanorods (GNRs) as plasmonic metal nanoparticles are valuable for optical applications due to their tunable plasmonic properties. However, conventional colloidal GNRs face significant optical instability during storage, which limits their practical use. In this work, we developed a highly dispersible GNR powder using an octadecyl trimethylammonium bromide (CTAB)-assisted freeze-drying method, preserving the optical and chemical sensing properties of GNRs for over 4 months.

Predicting Pancreatic Cancer in New-Onset Diabetes Cohort Using a Novel Model With Integrated Clinical and Genetic Indicators: A Large-Scale Prospective Cohort Study.

Introduction: Individuals who develop new-onset diabetes have been identified as a high-risk cohort for pancreatic cancer (PC), exhibiting an incidence rate nearly 8 times higher than the general population. Hence, the targeted screening of this specific cohort presents a promising opportunity for early pancreatic cancer detection. We aimed to develop and validate a novel model capable of identifying high-risk individuals among those with new-onset diabetes.

An inverted U-shaped relationship between work-family conflict and job burnout among village clinic doctors: the moderating effect of information and communication technology use.

Background: Village clinic doctors are an integral part of the rural healthcare workforce. With the advancement of science and technology and the coronavirus disease 2019 (COVID-19) pandemic, the prevalence of remote work and the use of information and communication technologies (ICT) have increased significantly. However, these developments have also had an impact on the work family balance and mental well-being of village clinic doctors.

[Establishment of BCL-2 Inhibitors-Resistant B-cell Acute Lymphoblastic Leukemia Cell Lines and Study on Their Resistance Mechanisms].

Objective: RS4;11 cell line was used to establish BCL-2 inhibitor-resistant cell lines of B-cell acute lymphoblastic leukemia (B-ALL) and explore the possible mechanisms of drug resistance.

Methods: RS4;11 cell line was continuously induced and cultured by low and ascending concentrations of BCL-2 inhibitors navitoclax and venetoclax to construct navitoclax-resistant cell line RS4;11/Nav and venetoclax-resistant cell line RS4;11/Ven. The cell viability was detected by MTT assay, and the cell apoptosis was detected by flow cytometry.