KAT7 serves as an oncogenic gene and regulates CCL3 expression via STAT1 signaling in osteosarcoma.

Osteosarcoma, considered as the primary cause of malignant bone tumors in children, necessitates novel therapeutic strategies to enhance overall survival rates. KAT7, a histone acetyltransferase, exerts pivotal functions in gene transcription and immune modulation. In light of this, our study identified a significant upregulation of KAT7 in the mRNA and protein levels in human osteosarcoma, boosting cell proliferation in vivo and in vitro.

Modifying soil bacterial communities in saline mudflats with organic acids and substrates.

Aims: The high salinity of soil, nutrient scarcity, and poor aggregate structure limit the exploitation and utilization of coastal mudflat resources and the sustainable development of saline soil agriculture. In this paper, the effects of applying exogenous organic acids combined with biological substrate on the composition and diversity of soil bacterial community were studied in moderately saline mudflats in Jiangsu Province.

Methods: A combination of three exogenous organic acids (humic acid, fulvic acid, and citric acid) and four biological substrates (cottonseed hull, cow manure, grass charcoal, and pine needle) was set up set up on a coastal saline mudflat planted with a salt-tolerant forage grass, sweet sorghum.

Synthesis and Characterization of Photo-Cross-Linkable Silk Fibroin Methacryloyl Hydrogel for Biomedical Applications.

Photo-cross-linkable hydrogels have recently gained increased interest in the field of biomedical applications. In this study, silk fibroin was derivatized with methacrylic anhydride (MA) to obtain silk fibroin methacryloyl (SFMA), forming hydrogel under UV light exposure in 1 min. The SFMA sol-gel transition did not involve significant structural change at the early stage.

MATN3 delivered by exosome from synovial mesenchymal stem cells relieves knee osteoarthritis: Evidence from and studies.

Background: Synovial mesenchymal stem cell (SMSC) exerts chondroprotective effects in osteoarthritis (OA) clinical models. However, the regulatory potentials of SMSC-derived exosomes (SMSC-Exo) in OA still need to be discovered, which attracted our attention.

Methods: The destabilization of the medial meniscus surgery was performed on the knee joints of a mouse OA model, followed by injection of SMSC-Exo.

Hounsfield Unit for Evaluating Bone Mineral Density and Strength: Variations in Measurement Methods.

Objective: To assess the consistency and accuracy of various measurements of the Hounsfield unit (HU) in lumbar vertebrae.

Methods: The study reviewed lumbar spine computed tomography images of 60 postmenopausal women aged >50 years. A total of 240 vertebrae were measured and analyzed for the variations of HU values in different sections and regions.

Hp0521 inhibited the virulence of 26,695 strain via regulating CagA expression.

is the number of cag pathogenicity island (cagPAI) family in (), which encoded Cag2 protein. The aim of this study was to investigate the role of on the strain. We constructed the recombinant prokaryotic expression plasmid pET-32a- and pET-32a-.

Biomass composite with exogenous organic acid addition supports the growth of sweet sorghum ( '') by reducing salinity and increasing nutrient levels in coastal saline-alkaline soil.

Introduction: In coastal saline lands, organic matter is scarce and saline stress is high. Exploring the promotion effect of intervention with organic acid from biological materials on soil improvement and thus forage output and determining the related mechanism are beneficial to the potential cultivation and resourceful, high-value utilization of coastal mudflats as back-up arable land.

Method: Three exogenous organic acids [humic acid (H), fulvic acid (F), and citric acid (C)] were combined with four kinds of biomass materials [cottonseed hull (CH), cow manure (CM), grass charcoal (GC), and pine needle (PN)] and applied to about 0.

Vertebral bone quality score provides preoperative bone density assessment for patients undergoing lumbar spine surgery: a retrospective study.

Objective: The novel MRI-based vertebral bone quality (VBQ) score has been described as an opportunistic screening tool for osteoporosis, but the stability and practical value of this score deserve further investigation. The purpose of this study was to assess whether preoperative VBQ scores could assist in identifying reduced bone mineral density (BMD) or osteoporosis and evaluating the consistency between MRI systems with different field strengths.

Methods: The VBQ scores of the patients who underwent surgery for lumbar disc herniation and the single-level VBQ scores of each L1-4 vertebral body were measured and calculated with preoperative lumbar MRI noncontrast T1-weighted phases.

Analysis of the Curative Effect of Curved Angle Vertebroplasty in the Treatment of Osteoporotic Vertebral Compression Fracture.

Objective: To evaluate the clinical efficacy of Percutaneous curved vertebroplasty (PCVP) for osteoporotic vertebral compression fracture of the thoracolumbar spine.

Methods: Retrospective analysis of 113 patients with osteoporotic vertebral compressive fractures (OVCFs) in our hospital from January 2017 to January 2020, a total of 120 diseased vertebrae, were divided into PCVP group (35 cases, 37 sections) and bilateral PVP(BVP) group (78 cases, 83 sections). To compare the distribution of baseline clinical data, pain relief (Visual Analog Scale, VAS), ODI (Oswestry Dability Index, ODI), operation time, intraoperative fluoroscopy, postoperative vertebral body re-fracture, and comparison of bone, and to compare the volume of cement penetration and the leakage rate of bone cement, etc.

Pyridine-linked covalent triazine frameworks with bidirectional electron donor-acceptor for efficient organic pollution removal.

Simultaneous regulation of adsorption and photocatalytic performance of covalent triazine frameworks (CTFs) to achieve efficient control of organic pollution in water is a promising strategy, but remains a formidable challenge. Herein, pyridine linkers were innovatively introduced into pristine CTF (p-CTF) and the bidirectional electron donor-acceptor (EDA) system of contaminant-to-pyridine and pyridine-to-triazine was constructed inside. Experimental results combined with theoretical calculations revealed that pyridine units with π-deficient properties performed as electron acceptors and electron donors in the adsorption and photocatalytic processes, respectively.

Systematic Identification and Expression Analysis of the Sorghum Gene Family Reveals Several New Members Encoding High-Affinity Phosphate Transporters.

Sorghum () is known to have a more robust capability of phosphorus uptake than many other cereal plants, which could be attributed to its phosphate transporter 1 (Pht1) that has a high phosphorus affinity. There are eleven genes in the sorghum genome, nine of which are expressed in sorghum roots or shoots in response to phosphorus deficiency (low-P). The molecular features of these nine genes were investigated by gene expression analysis, subcellular localization, and a yeast mutant complementation growth assay.

biocompatibility study of EDC/NHS cross-linked silk fibroin scaffold with olfactory ensheathing cells.

In this paper, we investigated silk fibroin (SF) cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) and its biocompatibility with olfactory ensheathing cells (OECs). After cross-linked with different concentrations of EDC/NHS solutions, SF scaffolds were analyzed by different techniques such as scanning electron microscopy, Fourier transform infrared spectra, x-ray diffraction, tensile machine and water contact angle assay. As to their structures, we found 4.

The deer play in Wuqinxi and four-point hand-knee kneeling positions for training core muscle function and spinal mobility.

The four-point kneeling exercise is a core stabilization exercise that provides the spine with dynamic stability and neuromuscular control. In the traditional Chinese exercise Wuqinxi, deer play is performed in a hand-foot kneeling (HFK) position, which is remarkably similar to the four-point hand-knee kneeling (HKK) position. However, the differences in spinal function promotion between these two positions are poorly understood.

Mechanical analysis of deep tissue injury during sitting in patients with spinal cord injury via parametric finite element model.

Spinal cord injury patients are prone to develop deep tissue injury because of long-term mechanical load. However, there is a lack of statistical research on the influence of tissue characteristics on the internal mechanical state of soft tissue. This study aimed to investigate the influence of tissue characteristics on the internal mechanical state of buttock in spinal cord injury patients.

Shade Delayed Flowering Phenology and Decreased Reproductive Growth of L.

Alfalfa ( L.) is an important forage in intercropping or rotation ecosystem, and shading is the principal limiting factor for its growth under the crop or forest. Agronomic studies showed that shading would systematically reduce the biomass of alfalfa.

lncRNA LINC00284 promotes nucleus pulposus cell proliferation and ECM synthesis via regulation of the miR‑205‑3p/Wnt/β‑catenin axis.

Intervertebral disc degeneration (IDD) is a leading cause of degenerative spinal disease. Long non‑coding RNA (lncRNA) LINC00284 is overexpressed in multiple types of cancer and promotes cancer cell proliferation and inhibits apoptosis; however, its role in human IDD and nucleus pulposus (NP) remain unclear. In the present study, intervertebral disc (IVD) tissues were collected from IDD patients for detection of LINC00284 expression using reverse transcription‑quantitative PCR, the binding effect between miR‑205‑3p and LINC00284 was validated by dual‑luciferase reporter assay.

Synergism Between Water Management and Phosphorus Supply Enhances the Nodulation and Root Growth and Development of Chinese Milk Vetch ( L.).

The response of root development and nodule formation of the manure crop Chinese milk vetch to different levels of soil moisture and phosphorous (P) fertilizer remains unclear. In this study, a pot experiment was performed to trace the root growth and nodule formation of Chinese milk vetch at the seedling, branching and full-flowering stages, under various soil moisture gradients [25% (W1), 50% (W2), 75% (W3), and 100% (W4) of the maximum field water-holding capacity] and P levels [0 (P0), 30 (P1), 60 (P2), and 90 (P3) kg hm]. The root/shoot ratio, root vitality, number of nodules, nodule weight, and nitrogenase activity were affected remarkably by soil moisture or the level of added P across the whole stage.

Identification of Candidate Forage Yield Genes in Sorghum ( L.) Using Integrated Genome-Wide Association Studies and RNA-Seq.

Genetic dissection of forage yield traits is critical to the development of sorghum as a forage crop. In the present study, association mapping was performed with 85,585 SNP markers on four forage yield traits, namely plant height (PH), tiller number (TN), stem diameter (SD), and fresh weight per plant (FW) among 245 sorghum accessions evaluated in four environments. A total of 338 SNPs or quantitative trait nucleotides (QTNs) were associated with the four traits, and 21 of these QTNs were detected in at least two environments, including four QTNs for PH, ten for TN, six for SD, and one for FW.

Implantation of nanofibrous silk scaffolds seeded with bone marrow stromal cells promotes spinal cord regeneration (6686 words).

Spinal cord injury (SCI) is a common pathology often resulting in permanent loss of sensory, motor, and autonomic function. Numerous studies in which stem cells have been transplanted in biomaterial scaffolds into animals have demonstrated their considerable potential for recovery from SCI. In the present study, a three-dimensional porous silk fibroin (SF) scaffold with a mean pore size of approximately 383 μm and nanofibrous structure was fabricated, the silk scaffold enabling the enhanced attachment and proliferation of bone marrow stromal cells (BMSCs).