Bismuth Nano-Nest/TiCN Quantum Dot-Based Surface Plasmon Coupling Electrochemiluminescence Sensor for Ascites miRNA-421 Detection.

In this study, a novel surface plasmon-coupled electrochemiluminescence (SPC-ECL) biosensor was developed based on bismuth nano-nest and TiCN quantum dots (TiCN QDs). First, MXene derivative QDs (TiCN QDs) with excellent luminescence performance were prepared as the ECL luminescent. The N doping in TiCN QDs can effectively improve the luminescence performance and catalytic activity.

Accurate Capture and Identification of Exosomes: Nanoarchitecture of the MXene Heterostructure/Engineered Lipid Layer.

Recently, exosome detection has become an important breakthrough in clinical diagnosis. However, the effective capture and accurate identification of cancer exosomes in a complex biomatrix are still a tough task. Especially, the large size and non-conductivity of exosomes are not conducive to highly sensitive electrochemical or electrochemiluminescence (ECL) detection.

MoS QDs-MXene heterostructure-based ECL sensor for the detection of miRNA-135b in gastric cancer exosomes.

Exosomes play an important role in the proliferation, adhesion and migration of cancer cells. In this study, we have developed a novel electrochemiluminescence (ECL) sensor based on MoS QDs-MXene heterostructure and Au NPs@biomimetic lipid layer to detect exosomal miRNA. MoS QDs-MXene heterostructure had been prepared as the luminescence probe.

3D Plasmonic Nanostructure-Based Polarized ECL Sensor for Exosome Detection in Tumor Microenvironment.

Exosomes of cancer cells play an important role in the proliferation, adhesion, and migration of tumors. Especially, exosomes in the tumor microenvironment can reflect the proliferation of tumors directly, thus serving as ideal referenced markers of the possibility and grade of malignancy in neoplasms. However, the sensitive and accurate detection of exosomes remains challenging.

In situ synthesis of Cu nanoclusters/CeO nanorod as aggregated induced ECL probe for triple-negative breast cancer detection.

Cu nanoclusters (NCs) have attracted a lot of attention due to the excellent properties. However, the low luminescence and poor stability limited the Cu NC-based sensing research. In this work, Cu NCs were in situ synthesized on CeO nanorods.

The controllable assembly of Cu nanocluster-based aggregation induced ECL strategy for miRNA detection.

Copper nanoclusters (Cu NCs) were a new class of non-toxic and economical nanoprobe. However, the low luminescence performance and instability of Cu NCs limited the actual application. Herein, this work developed the novel controllable assembly of Cu NCs aggregation as the electrochemiluminescence (ECL) emitter.

Confined Gold Single Atoms-MXene Heterostructure-Based Electrochemiluminescence Functional Material and Its Sensing Application.

Herein, based on electronic metal-support interaction (EMSI), a gold single atom confined MXene (Au/MXene) heterostructure was developed as the highly efficient electrochemiluminescence (ECL) functional material, which greatly improved the electrochemical properties and broadened the sensing application of MXenes. Gold single atoms were confined into the vacancy defects of TiCT MXene, which could effectively avoid the masking of catalytic active sites. Meanwhile, electron transport could be accelerated with the assistance of titanium dioxide on the MXene nanosheets.

A novel nanosponge-hydrogel system-based electrochemiluminescence biosensor for uric acid detection.

In this work, a highly efficient electrochemiluminescence (ECL) biosensor was developed based on the nanosponge-hydrogel system for uric acid (UA) detection. First, the nanosponge consisted of polylactic acid glycolic acid (PLGA) nanoparticles immobilized with MoS quantum dots (QDs) and urate oxidase (UAO). The marked loading capability of PLGA nanoparticles enables loading many biomolecules and QDs for the specific recognition of UA.

A practical guide to scanning light-field microscopy with digital adaptive optics.

With the development of a wide variety of animal models in recent years, there is a rapidly growing demand for long-term, high-speed intravital fluorescence imaging to observe intercellular and intracellular interactions in their native states. Scanning light-field microscopy (sLFM) with digital adaptive optics provides a compact computational solution by imaging the entire volume in a tomographic way with orders-of-magnitude improvement in spatiotemporal resolution and reduction in phototoxicity, as compared to traditional intravital microscopy. Here, we present a step-by-step protocol for both hardware and software implementation of multicolor sLFM as an add-on to a normal wide-field fluorescence microscope by using off-the-shelf lenses and devices at low cost.

Construction of a Sensing Platform Based on DNA-Encoded Magnetic Beads and Copper Nanoclusters for Viral Gene Analysis with Target Recycling Amplification.

The rapid and accurate monitoring of viral genes plays an important role in the area of disease diagnosis, biomedical research, and food safety. Herein, we successfully designed a sensing system that combined the technologies of target DNA recycling amplification, magnetic separation, and in situ formation of fluorescent copper nanoclusters (CuNCs) for viral DNA analysis. In the presence of target viral DNA (tDNA), a large quantity of output DNA (oDNA) was produced from hairpin DNA (hDNA) through an exonuclease III-assisted target recycling amplification strategy.

Gold Nanorod Vertical Array-Based Electrochemiluminescence Polarization Assay for Triple-Negative Breast Cancer Detection.

In this work, a polarization-resolved electrochemiluminescence (ECL) sensor for microRNA-155 (miRNA-155) detection has been constructed based on the surface plasmon coupling effect. In the sensing system, nitrogen dots (N dots) were employed as ECL emitters. As a surface-enhanced structure, a gold nanorod vertical array was constructed on the electrode surface by the volatilization-induced self-assembly.

MXene-Derived Quantum Dot@Gold Nanobones Heterostructure-Based Electrochemiluminescence Sensor for Triple-Negative Breast Cancer Diagnosis.

MXene material has been gradually studied in recent years due to its fascinating characteristics. This work developed a novel MXene-derived quantum dots (MQDs) @gold nanobones (Au NBs) heterostructure as the electrochemiluminescence (ECL) sensor. First, MXene and MQDs were synthesized via the green preparation process, which avoided the harm of hydrofluoric acid to humans and the environment.

Polarization-Resolved Electrochemiluminescence Sensor Based on the Surface Plasmon Coupling Effect of a Au Nanotriangle-Patterned Structure.

This work focused on the construction of a nanomaterial-patterned structure for high-resolved ECL signal modulation. Due to the surface coupling effect, the different shapes and distribution states of surface plasmonic nanomaterials not only affect the luminescence intensity enhancement but also decide the electrochemiluminescence (ECL) polarization characteristics. Herein, tin disulfide quantum dots were synthesized via a solvothermal method as ECL emitters.

Luminous MoS nanosheet-based electrochemiluminescence biosensor with biomimetic vesicle for miRNA-210 detection.

In this work, a novel electrochemiluminescence (ECL) sensor has been developed to detect miRNA-210 in the serum of triple negative breast cancer (TNBC) patients. The luminous MoS nanosheets were synthesized via the solvothermal method and served as ECL emitters for the first time. As a result, the ECL properties of as-prepared MoS nanosheets were significantly improved.

Multiplex Electrochemiluminescence Polarization Assay Based on the Surface Plasmon Coupling Effect of Au NPs and Ag@Au NPs.

A novel multiplex electrochemiluminescence (ECL) polarization assay was developed to detect breast cancer-related genes BRCA1 and BRCA2 simultaneously based on the polarization characteristics of surface plasmon-coupled electrochemiluminescence (SPC-ECL). In this work, boron nitride quantum dots (BN QDs) were used as ECL emitters, and gold nanoparticles (Au NPs) and gold-coated silver nanoparticles (Ag@Au NPs) were employed as surface plasmon materials. The surface plasmon coupling resonance of different metal NPs not only enhanced the ECL intensity but also converted the isotropic emission into directional emission.

Rational Fabrication of a Smart Electrochemiluminescent Sensor: Synergistic Effect of a Self-Luminous Faraday Cage and Biomimetic Magnetic Vesicles.

A smart electrochemiluminescent (ECL) sensor has been designed in this work. The sensing system consisted of Ag NPs-TiAlC nanosheets (Ag-TACS) as the self-luminous Faraday cage and biomimetic magnetic vesicles as the functional substrate. By engineering the structure and properties of TiAlC nanosheets to induce the Faraday-cage effect, the outer Helmholtz plane (OHP) was extended to contribute to ECL enhancement.

AgPO NP@MoS nanosheet enhanced F, S-doped BN quantum dot electrochemiluminescence biosensor for K-ras tumor gene detection.

In this research, a novel AgPO NPs@MoS nanosheet-based electrochemiluminescence (ECL) sensing system was developed to provide an effective method for tumor gene detection. At first, fluorine, sulfur-doped BN quantum dot (F, S-BN QD) were prepared as ECL emitter. Sulfur dopant can provide more reactive sites in the ECL reaction.

Tunable plasmon-assisted electrochemiluminescence strategy for determination of the rapidly accelerated fibrosarcoma B-type (BRAF) gene using concave gold nanocubes.

A tunable plasmon-assisted electrochemiluminescence (ECL) strategy is reported using concave Au nanocubes (Au CBs) for rapidly accelerated fibrosarcoma B-type (BRAF) detection. Concave Au CBs exhibit a strong surface plasmon coupling (SPC) effect between its sharp apexes and edges. The high spectral overlap with graphite phase carbon nitride quantum dots (g-CN QDs) is achieved by tuning surface plasmon absorption peak of the concave Au CBs.

FeO NP@ZIF-8/MoS QD-based electrochemiluminescence with nanosurface energy transfer strategy for point-of-care determination of ATP.

Herein, FeO NP@ZIF-8/MoS QD-based electrochemiluminescence (ECL) biosensor with nanosurface energy transfer strategy was successfully developed for point-of-care determination of ATP. With the porous structure and poor electron transfer ability, FeO NP@ZIF-8 complex was first used as an excellent catalyst in ECL. The complex catalyzed the coreactant for more free radicals and hindered the quenching effect of FeO nanoparticles (NPs) on quantum dots (QDs).

Polarized-Electrochemiluminescence Biosensor Based on Surface Plasmon Coupling Strategy and Fluorine-Doped BN Quantum Dots.

The first polarized-electrochemiluminescence (ECL) biosensor is reported in this work. Surface plasmon coupling ECL (SPC-ECL) strategy is developed for the amplified polarization light of fluorine-doped BN quantum dot (F-BN QD) emitters. The generation of polarized-ECL is attributed to the characteristic of polarization-angle-dependent SPC effect.

A novel high efficient electrochemiluminescence sensor based on reductive Cu(I) particles catalyzed Zn-doped MoS QDs for HPV 16 DNA determination.

In this work, we explored a high efficient electrochemiluminescence (ECL) sensor based on the synergistic enhancement strategy of Zn-doped MoS quantum dots (QDs) and reductive Cu(I) particles. On the one hand, Zn-doped MoS QDs with sulfur vacancies were designed to improve the ECL activity of QDs. The regulated sulfur vacancies with zinc doping resulted in adsorption and coordination with transition metals of HO as coreactant.

A Visual FRET Immunofluorescent Biosensor for Ratiometric Parathyroid Hormone (1-84) Antigen Point-of-Care Detection.

Excessive secretion of PTH leads to disturbance of calcium and phosphorus metabolism in the body, which promotes bone, kidney, digestive system and nervous system diseases. Due to the short half-life of PTH, it becomes a difficult issue for PTH detection in the clinical diagnosis field. We explored a competitive immunofluorescent sensing mode based on FRET of two-color CdTe QDs for ratiometric PTH 1-84 antigen detection.

A visual electrochemiluminescence biosensor based on CuInZnS quantum dots for superoxide dismutase detection.

Superoxide dismutase (SOD), also known as liver protein, is a substance widely distributed in various biological cells. It has the function of catalyzing the disproportionation reaction of superoxide free radicals. SOD can form an antioxidant chain together with peroxidase, catalase, and other substances in the body of organisms, and thus, is one of the indispensable important substances in the body of organisms.

Distance-dependent plasmon-enhanced electrochemiluminescence biosensor based on MoS nanosheets.

Nonmetallic plasmonic MoS nanosheets were synthesized by hydrothermal top-down method. MoS nanosheets had shown strong surface plasmon coupling (SPC) light absorption in the visible and near-infrared region. Herein, the nonmetallic plasmonic MoS nanosheets were employed to enhance the electrochemiluminescence (ECL) signal of sulfur doped boron nitrogen QDs (S-BN QDs) in this work.

Wavelength-Dependent Surface Plasmon Coupling Electrochemiluminescence Biosensor Based on Sulfur-Doped Carbon Nitride Quantum Dots for K-RAS Gene Detection.

Although graphite phase carbon nitride quantum dots (GCN QDs) showed some advantages in the electrochemiluminescence (ECL) analytical research, the low ECL efficiency limited the potential sensing application. Herein, we synthesized sulfur-doped graphite phase carbon nitride quantum dots (S-GCN QDs) to fabricate a sandwich sensor based on amplified surface plasmon coupling ECL (SPC-ECL) mode. Sulfur doping can change the surface states of QDs effectively and produced new element vacancy.