Human placental mesenchymal stem cells ameliorates premature ovarian insufficiency via modulating gut microbiota and suppressing the inflammation in rats.

The core objective of this study was to explore the effects and potential mechanism of human placental mesenchymal stem cells (PMSCs) in improving early-onset ovarian dysfunction (POI). Mesenchymal stem cells with multidirectional differentiation ability were isolated from human placenta tissue and a culture system of human PMSCs was constructed for this study. Subsequently, we successfully constructed POI rat models using cisplatin induction.

Electrochemical pH Sensor Incorporated Wearables for State-of-the-Art Wound Care.

Nonhealing chronic wounds pose severe physiological and psychological distress to patients, making them a significant concern for global public health. Effective wound management strategies assisted by smart wearable pH monitoring have the potential to substantially alleviate both social and economic burdens. The pH of the wound exudate serves as a valuable indicator for predicting infections and assessing the healing status of wounds.

MambaSAM: A Visual Mamba-Adapted SAM Framework for Medical Image Segmentation.

The Segment Anything Model (SAM) has shown exceptional versatility in segmentation tasks across various natural image scenarios. However, its application to medical image segmentation poses significant challenges due to the intricate anatomical details and domain-specific characteristics inherent in medical images. To address these challenges, we propose a novel VMamba adapter framework that integrates a lightweight, trainable Visual Mamba (VMamba) branch with the pre-trained SAM ViT encoder.

E-twenty-six-specific sequence variant 5 (ETV5) facilitates hepatocellular carcinoma progression and metastasis through enhancing polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC)-mediated immunosuppression.

Background: Despite the success of immune checkpoint blockade, a lack of understanding of the hepatocellular carcinoma (HCC) immune microenvironment impedes its development.

Objective: We aim to elucidate the essential function of E-twenty-six-specific sequence variant 5 (ETV5) in regulating the immune microenvironment in HCC.

Design: Humanised mouse models, murine orthotopic models and diethylnitrosamine/carbon tetrachloride (DEN/CCl)-induced HCC models were used to examine the function of ETV5.

Fully Integrated Centrifugal Microfluidics for Rapid Exosome Isolation, Glycan Analysis, and Point-of-Care Diagnosis.

Exosomes present in the circulatory system demonstrate considerable promise for the diagnosis and treatment of diseases. Nevertheless, the complex nature of blood samples and the prevalence of highly abundant proteins pose a significant obstacle to prompt and effective isolation and functional evaluation of exosomes from blood. Here, we present a fully integrated lab-on-a-disc equipped with two nanofilters, also termed iExoDisc, which facilitates automated isolation of exosomes from 400 μL blood samples within 45 min.

Effect of the recombinant amelogenin peptide TRAP on remineralization of early enamel caries: an in-situ study.

Background: The tyrosine-rich amelogenin peptide (TRAP) is the main enzymatic hydrolysis product of amelogenin during enamel development. The TRAP peptide has been shown to promote the remineralization of early enamel caries in vitro. The aim of this crossover blinded and randomized study was to evaluate the impact of recombinant amelogenin peptide TRAP on the remineralization of initial enamel caries lesions in situ.

Causal enhancement of cognitive reappraisal through synchronized dorsolateral and ventrolateral prefrontal cortex activity.

The dorsolateral prefrontal cortex (DLPFC) and ventrolateral prefrontal cortex (VLPFC) are central to cognitive reappraisal, yet the mechanisms underlying their collaboration remain unclear. This study uses transcranial alternating current stimulation (tACS) to modulate neural synchrony between the DLPFC and VLPFC in the theta band, aiming to elucidate the effects of synchronous versus asynchronous neural interaction on reappraisal. It also examines whether these effects are unique to reappraisal or extend to other emotion regulation strategies.

Freeze-Thaw Imaging for Microorganism Classification Assisted with Artificial Intelligence.

Fast and cost-effective microbial classification is crucial for clinical diagnosis, environmental monitoring, and food safety. However, traditional methods encounter challenges including intricate procedures, skilled personnel needs, and sophisticated instrumentations. Here, we propose a cost-effective microbe classification system, also termed freeze-thaw-induced floating pattern of AuNPs (FTFPA), coupled with artificial intelligence, which is capable of identifying microbes at a cost of $0.

Online Health Information Seeking and Cyberchondria Among Men at Risk of HIV Infection: A Moderated Mediation Model.

Objectives: This study investigates the relationship between HIV/AIDS-related online health information seeking (OHIS) and cyberchondria among Chinese men who have engaged in high-risk sexual behaviors. It proposes a moderated mediation model to explore the role of query escalation as a mediator and intolerance of uncertainty (IU) as a moderator in this relationship.

Method: A survey was conducted with 227 men from an online community focused on HIV/AIDS-related fear in China.

fNIRS neurofeedback facilitates emotion regulation: Exploring individual differences over the ventrolateral prefrontal cortex.

The ventrolateral prefrontal cortex (VLPFC) plays a pivotal role in emotion regulation, yet the effectiveness of neurofeedback (NF) training targeting the VLPFC remains uncertain, suggesting significant individual differences in outcomes. In this study, we aimed to clarify these differences by enrolling 90 participants, randomly assigned to either an experimental group or a sham group (n = 48/42). Participants in the experimental group underwent VLPFCNF training over eight sessions across two consecutive days, while those in the sham group received random signals from functional near-infrared spectroscopy (fNIRS).

Lactobacillus rhamnosus GG ameliorates atherosclerosis via suppression of oxidative stress and inflammation by reshaping the gut microbiota.

Objective: With growing awareness of probiotics' benefits, more studies are exploring their efficacy and mechanisms in reducing atherosclerosis (AS). This study aimed to investigate the potential therapeutic effects of Lactobacillus rhamnosus GG (LGG) on atherosclerotic mice and underlying mechanisms.

Design: ApoE mice were gavaged with a dose of 2 × 10 CFU LGG per mouse once daily, while both ApoE and C57BL/6J mice received normal saline as controls.

Data-Driven Theoretical Modeling of Centrifugal Step Emulsification and Its Application in Comprehensive Multiscale Analysis.

Tailored droplet generation is crucial for droplet microfluidics that involve samples of varying sizes. However, the absence of precise predictive models forces droplet platforms to rely on empiricism derived from extensive experiments, underscoring the need for comprehensive modeling analysis. To address this, a novel customized assembled centrifugal step emulsifier (CASE) is presented by incorporating a "jigsaw puzzles" design to efficiently acquire large-scale experimental data.

Long Non-Coding RNAs: Crucial Regulators in Alzheimer's Disease Pathogenesis and Prospects for Precision Medicine.

Long non-coding RNAs (LncRNAs) have emerged as pivotal regulators in the pathogenesis of Alzheimer's disease (AD), a progressive neurodegenerative disorder characterized by cognitive decline and memory loss. With the capacity to modulate gene expression at various levels, LncRNAs are implicated in multiple pathological mechanisms of AD, including amyloid-beta (Aβ) accumulation, tau protein phosphorylation, neuroinflammation, and neuronal apoptosis. Recent studies have highlighted the potential of LncRNAs as diagnostic biomarkers and therapeutic targets due to their differential expression patterns in AD patients.

Artificial intelligence enhanced microfluidic system for multiplexed point-of-care-testing of biological thiols.

Cysteamine (CA) serves as a cystine-depleting agent employed in the management of cystinosis and a range of other medical conditions. Monitoring blood CA levels at the point of care is imperative due to the risk of toxicity associated with elevated CA dosages. An additional significant challenge is presented by the intricate composition of human plasma and the presence of various interfering biological thiols, which possess similar structures or properties.

Decoding cortical folding patterns in marmosets using machine learning and large language model.

Macroscale neuroimaging results have revealed significant differences in the structural and functional connectivity patterns of gyri and sulci in the primate cerebral cortex. Despite these findings, understanding these differences at the molecular level has remained challenging. This study leverages a comprehensive dataset of whole-brain in situ hybridization (ISH) data from marmosets, with updates continuing through 2024, to systematically analyze cortical folding patterns.

Unveiling a Tunable Moiré Bandgap in Bilayer Graphene/hBN Device by Angle-Resolved Photoemission Spectroscopy.

Over the years, great efforts have been devoted in introducing a sizable and tunable band gap in graphene for its potential application in next-generation electronic devices. The primary challenge in modulating this gap has been the absence of a direct method for observing changes of the band gap in momentum space. In this study, advanced spatial- and angle-resolved photoemission spectroscopy technique is employed to directly visualize the gap formation in bilayer graphene, modulated by both displacement fields and moiré potentials.

Recent advances in centrifugal microfluidics for point-of-care testing.

Point-of-care testing (POCT) holds significant importance in the field of infectious disease prevention and control, as well as personalized precision medicine. The emerging microfluidics, capable of minimal reagent consumption, integration, and a high degree of automation, play a pivotal role in POCT. Centrifugal microfluidics, also termed lab-on-a-disc (LOAD), is a significant subfield of microfluidics that integrates crucial analytical steps onto a single chip, thereby optimizing the process and enabling high-throughput, automated analysis.

Novel oral compound Z526 mitigates cancer-associated cachexia via intervening NF-κB signaling and oxidative stress.

Cancer-associated cachexia (CAC) is a severe metabolic disorder syndrome mainly characterized by muscle and fat loss, which accounts for one-third of cancer-related deaths. No effective therapeutic approach that could fully reverse CAC is available. NF-κB signaling and oxidative stress play vital roles in both muscle atrophy and fat loss in CAC.

Heteropolyacid Ligands in Two-Dimensional Channels Enable Lithium Separation from Monovalent Cations.

Extracting lithium from salt lakes requires ion-selective membranes with customizable nanochannels. However, it remains a major challenge to separate alkali cations due to their same valences and similar ionic radius. Inspired by the K channel of KcsA K, significant progress has been made in adjusting nanochannel size to control the ion selectivity dominated by alkali cations dehydration.

Long non-coding RNAs are involved in the crosstalk between cancer-associated fibroblasts and tumor cells.

The proliferation of tumors is not merely self-regulated by the cancer cells but is also intrinsically connected to the tumor microenvironment (TME). Within this complex TME, cancer-associated fibroblasts (CAFs) are pivotal in the modulation of tumor onset and progression. Rich signaling interactions exist between CAFs and tumor cells, which are crucial for tumor regulation.

Spatially defined microenvironment for engineering organoids.

In the intricately defined spatial microenvironment, a single fertilized egg remarkably develops into a conserved and well-organized multicellular organism. This observation leads us to hypothesize that stem cells or other seed cell types have the potential to construct fully structured and functional tissues or organs, provided the spatial cues are appropriately configured. Current organoid technology, however, largely depends on spontaneous growth and self-organization, lacking systematic guided intervention.

Freeze-Thaw-Induced Patterning of Extracellular Vesicles with Artificial Intelligence for Breast Cancers Identifications.

Extracellular vesicles (EVs) play a crucial role in the occurrence and progression of cancer. The efficient isolation and analysis of EVs for early cancer diagnosis and prognosis have gained significant attention. In this study, for the first time, a rapid and visually detectable method termed freeze-thaw-induced floating patterns of gold nanoparticles (FTFPA) is proposed, which surpasses current state-of-the-art technologies by achieving a 100 fold improvement in the limit of detection of EVs.

Strong electron-phonon coupling in magic-angle twisted bilayer graphene.

The unusual properties of superconductivity in magic-angle twisted bilayer graphene (MATBG) have sparked considerable research interest. However, despite the dedication of intensive experimental efforts and the proposal of several possible pairing mechanisms, the origin of its superconductivity remains elusive. Here, by utilizing angle-resolved photoemission spectroscopy with micrometre spatial resolution, we reveal flat-band replicas in superconducting MATBG, where MATBG is unaligned with its hexagonal boron nitride substrate.

Alterations of gut microbiota and metabolome are associated with primary nephrotic syndrome in children.

Background: Primary nephrotic syndrome (PNS) is a common glomerular disease in children. Dysbiosis of gut microbiota acts as a cause of Treg abnormalities. However, the intestinal metabolic impact of PNS with children remains poorly understood.