A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain.

Accumulating evidence supports the notion that microglia play versatile roles in different chronic pain conditions. However, therapeutic strategies of chronic pain by targeting microglia remain largely overlooked. This study seeks to develop a miRNA-loaded nano-delivery system by targeting microglia, which could provide a decent and long-lasting analgesia for chronic pain.

Epigenetic combined with transcriptomic analysis of the m6A methylome after spared nerve injury-induced neuropathic pain in mice.

Epigenetic changes in the spinal cord play a key role in the initiation and maintenance of nerve injury-induced neuropathic pain. N6-methyladenosine (m6A) is one of the most abundant internal RNA modifications and plays an essential function in gene regulation in many diseases. However, the global m6A modification status of mRNA in the spinal cord at different stages after neuropathic pain is unknown.

Celastrol-encapsulated microspheres prepared by microfluidic electrospray for alleviating inflammatory pain.

Inflammatory pain is induced by trauma, infection, chemical stimulation, etc. It causes severe physical and psychological agony to patients. Celastrol has powerful anti-inflammatory property and has achieved good results in various inflammation-related diseases.

miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice.

Background: Inflammation often leads to the occurrence of chronic pain, and many miRNAs have been shown to play a key role in the development of inflammatory pain. However, whether miR-26a-5p relieves pain induced by inflammation and its possible mechanism are still unclear.

Methods: The complete Freund's adjuvant (CFA)-induced inflammatory pain mouse model was employed.

Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model.

Background: Mesenchymal stem cell (MSCs)-derived small Extracellular Vesicles (sEVs) are considered as a new cell-free therapy for pain caused by nerve injury, but whether human placental mesenchymal stem cell-derived sEVs relieve pain in sciatic nerve injury and its possible mechanism are still unclear. In this study, we investigated the roles of hPMSCs-derived sEVs and related mechanisms in neuropathic pain.

Methods: The spared nerve injury (SNI) mouse model was employed.

Effect of Celastrol on LncRNAs and mRNAs Profiles of Cerebral Ischemia-Reperfusion Injury in Transient Middle Cerebral Artery Occlusion Mice Model.

Celastrol plays a significant role in cerebral ischemia-reperfusion injury. Although previous studies have confirmed that celastrol post-treatment has a protective effect on ischemic stroke, the therapeutic effect of celastrol on ischemic stroke and the underlying molecular mechanism remain unclear. In the present study, focal transient cerebral ischemia was induced by transient middle cerebral artery occlusion (tMCAO) in mice and celastrol was administered immediately after reperfusion.

Experimental study on remediation of low permeability Cu-Zn contaminated clay by vacuum enhanced leaching combined with EDTA and hydrochloric acid.

To improve the washing efficiency for low permeable clay, the method of vacuum enhanced washing technology was proposed. In this study, ethylene diamine tetraacetic acid (EDTA) and hydrochloric acid (HCl) as eluents, the experiments of remediation of Cu-Zn contaminated cohesive soil by traditional soil column leaching and vacuum enhanced leaching was carried out. Results show that compared with traditional leaching method, the vacuum enhanced leaching method can significantly shorten the leaching time and prominently improve the removal rate of heavy metals Cu-Zn.

Seaweed farms provide refugia from ocean acidification.

Seaweed farming has been proposed as a strategy for adaptation to ocean acidification, but evidence is largely lacking. Changes of pH and carbon system parameters in surface waters of three seaweed farms along a latitudinal range in China were compared, on the weeks preceding harvesting, with those of the surrounding seawaters. Results confirmed that seaweed farming is efficient in buffering acidification, with Saccharina japonica showing the highest capacity of 0.

Experimental study on treating landfill sludge by preconditioning combined with vacuum preloading: Effects of freeze-thaw and FeCl preconditioning.

The deep dewatering of landfill sludge (LS) mainly uses the methods of chemical preconditioning and mechanical dewatering, which is easy to cause environmental pollution and is not conducive to the subsequent recycling treatment of sludge. To find a more environment-friendly and efficient method for LS's deep dewatering and volume reduction, an in-situ sludge treatment method combining freeze-thaw (F/T) preconditioning and vacuum preloading was proposed. Firstly, the F/T test of LS was carried out to explore the optimum freezing temperature.

Insight into conditioning landfill sludge with ferric chloride and a Fenton reagent: Effects on the consolidation properties and advanced dewatering.

The landfill sludge in storage reservoirs needs to be dewatered and disposed of for environmental and engineering purposes. The key factors are the high organic matter content and low permeability. Chemical conditioning is considered an efficient method for adjusting the properties of sludge.