Multi-pass, single-molecule nanopore reading of long protein strands.

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal. Here, we establish a method for the long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array.

[Spatial and Temporal Evolution and Prediction of Carbon Storage in Kunming City Based on InVEST and CA-Markov Model].

Land use/cover change is an important driving factor for carbon stock changes in terrestrial ecosystems and affects the carbon cycle of the whole ecosystem. Taking Kunming City as a case study, based on the modified carbon density coefficient, this study analyzed the spatio-temporal characteristics of carbon storage changes in the terrestrial ecosystem under different land use scenarios from 2000 to 2020 and "three-line" constraints by coupling the carbon storage module of the InVEST model and CA-Markov model. The results showed that:① cultivated land, forest land, and grassland were the main types of land use in Kunming City, and land use transfer also occurred among the three types.

Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity.

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal. Here, we establish a method for long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array.