Effects of high stocking density on the growth performance, intestinal health and bile salts composition of broiler chickens.

Introduction: The intestinal dysfunction plays an important role in the decreased growth performance of broiler chickens under high stocking density. Gut microbiota plays an important role in maintaining intestinal health. However, the modulation pathway of gut microbiota by regulating the intestinal barrier and histomorphology remains unknown.

The stems of Syringa oblata Lindl. exert cardioprotective effects against acute myocardial ischemia by inhibiting the TLR4/MyD88/NF-κB and NLRP3 inflammasome signaling pathways in mice.

Ethnopharmacological Relevance: The stripped roots and stems of Syringa oblata Lindl. (SO), a Mongolian and Tibetan folk medicinal plant, are renowned for their traditional use against "Khii", pain relief, and heat clearing. It is used to treat cardiovascular diseases (CVDs), upset, insomnia, and other symptoms and is commonly used as a substitute for another plant known as S.

Anti-inflammatory lignans from peeled stems of Syringa pinnatifolia.

Three new lignans namely alashanenols H-J (1-3) and one dimeric lignan namely bisyringol A (4) together with 15 known analogues (5-19) were isolated from the peeled stems of Syringa pinnatifolia Hemsl. with the aid of an in vitro anti-inflammation guided fractionation. The structures were elucidated by analysis of spectroscopic data, including IR, UV, MS, and NMR, and their absolute configurations were resolved by X-ray single crystal diffraction analysis and comparison of experimental and calculated C NMR and ECD data.

Hendersines J-M: Isoquinoline alkaloids from Corydalis hendersonii Hemsl. with cardiomyocyte protective and NO production inhibitory effects.

Six isoquinoline alkaloids were identified from the alkaloid-rich fraction of Corydalis hendersonii Hemsl, including five previously undescribed isoquinoline alkaloids hendersines J-M (1a, 1b, and 2-4) and isobicuculline (5), a compound reported for the first time from a natural source. Their structures were elucidated based on spectroscopic analysis of HR-ESI-MS, 1D and 2D NMR, X-ray diffraction, and ECD. Compounds 1a and 1b represent a pair of rare three-nitrogen isoquinoline alkaloid enantiomers, while 2 and 3 are isoquinoline alkaloids featuring a benzo-fused N-heterocycle.

TMEM232 is required for the formation of sperm flagellum and male fertility in mice.

Asthenoteratozoospermia is a major cause of male infertility. Thus far, the identified related genes can explain only a small share of asthenoteratozoospermia cases, suggesting the involvement of other genes. The transmembrane protein TMEM232 is highly expressed in mouse testes.

GSE1 promotes the proliferation and migration of lung adenocarcinoma cells by downregulating KLF6 expression.

Lung cancer is one of the most prevalent human cancers with a high lethality rate worldwide. In this study, we demonstrated that GSE1 (genetic suppressor element 1) expression is aberrantly upregulated in lung adenocarcinoma and that GSE1 depletion inhibits the proliferation and migration of both A549 and H1299 cells. Immunoprecipitation assays demonstrated that GSE1 interacts with histone deacetylase 1 (HDAC1) and other BRAF-HDAC complex (BHC) components in cells.

Unraveling the Impact of the PROCA1 Mutation in Male Infertility: Incorporating Whole Exome Sequencing in Teratozoospermia Patients and Analyzing Proca1 Knockout Mice.

In the world, about 15% of couples are infertile, and nearly half of all infertility was caused by men. A large number of genetic mutations are thought to affect spermatogenesis by regulating acrosome formation. Here, we identified three patients harbouring the protein interacting with cyclin A1 (PROCA1) mutation by whole exome sequencing (WES) and Sanger sequencing among patients with predominantly acrosome-deficient teratozoospermia.

Isolation and functional identification of immune cells in hemolymph of blood clams Tegillarca granosa.

Blood clam Tegillarca granosa is a type of economically cultivated bivalve mollusk with red blood, and it primarily relies on hemocytes in its hemolymph for immune defense. However, there are currently no reports on the isolation and identification of immune cells in T. granosa, which hinders our understanding of their immune defense.

Probiotic Nissle 1917 protect chicks from damage caused by serovar Enteritidis colonization.

As a foodborne pathogen of global importance, serovar Enteritidis ( Enteritidis) is a threat to public health that is mainly spread by poultry products. Intestinal Enterobacteriaceae can inhibit the colonization of Enteritidis and are regarded as a potential antibiotic substitute. We investigated, in chicks, the anti- Enteritidis effects of () Nissle 1917, the most well-known probiotic member of Enterobacteriaceae.

Proper phosphorylation of septin 12 regulates septin 4 and soluble adenylyl cyclase expression to induce sperm capacitation.

Septin-based ring complexes maintain the sperm annulus. Defective annular structures are observed in the sperm of Sept12- and Sept4-null mice. In addition, sperm capacitation, a process required for proper fertilization, is inhibited in Sept4-null mice, implying that the sperm annulus might play a role in controlling sperm capacitation.

The mediating role of resilience in the relationship between social support and quality of life among patients after radical cystectomy: A structural equation model analysis.

Aim: This study aimed to examine the relationship between social support and quality of life in urostomy patients and identify the mediating role of resilience in that relationship.

Design: A cross-sectional design.

Methods: Participants included 232 patients who were recruited from a tertiary hospital in Beijing during March 2020 and August 2020.

Making human pancreatic islet organoids: Progresses on the cell origins, biomaterials and three-dimensional technologies.

Diabetes is one of the most socially challenging health concerns. Even though islet transplantation has shown promise for insulin-dependent diabetes, there is still no effective method for curing diabetes due to the severe shortage of transplantable donors. In recent years, organoid technology has attracted lots of attention as organoid can mirror the human organ to the maximum extent thus bridging the gap between cellular- and tissue/organ-level biological models.

The role of self-efficacy in mediating between professional identity and self-reported competence among nursing students in the internship period: A quantitative study.

Aim: This study explored the relationship between self-efficacy, professional identity and competence among nursing students and analyzed the mediating role of self-efficacy in the relationship between professional identity and competence.

Background: Increasing attention has been paid to the cultivation of competence among nursing students; however, few studies to date have analyzed its related factors and examined their relationship.

Design: A quantitative study with a descriptive design was performed in this study, adopting an online survey with convenience and snowball sampling.

Evaluation of a Lecithin Supplementation on Growth Performance, Meat Quality, Lipid Metabolism, and Cecum Microbiota of Broilers.

The present study was conducted to evaluate the effects of lecithin on the performance, meat quality, lipid metabolism, and cecum microbiota of broilers. One hundred and ninety-two one-day-old AA broilers with similar body weights (38 ± 1.0 g) were randomly assigned to two groups with six replicates of sixteen birds each and were supplemented with 0 and 1 g/kg of lecithin for forty-two days.

Protein Kinase A-Mediated Septin7 Phosphorylation Disrupts Septin Filaments and Ciliogenesis.

Septins are GTP-binding proteins that form heteromeric filaments for proper cell growth and migration. Among the septins, septin7 (SEPT7) is an important component of all septin filaments. Here we show that protein kinase A (PKA) phosphorylates SEPT7 at Thr197, thus disrupting septin filament dynamics and ciliogenesis.

Correction to 'Cytotoxicity studies of FeO nanoparticles in chicken macrophage cells'.

[This corrects the article DOI: 10.1098/rsos.191561.

Cytotoxicity studies of FeO nanoparticles in chicken macrophage cells.

Magnetic FeO nanoparticles (FeO-NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of FeO-NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of FeO-NPs (60, 120 and 250 nm) were created, and the FeO-NPs were added to the cells at different doses according to the experimental group.

The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.

The connecting pieces of the sperm neck link the flagellum and the sperm head, and they are important for initiating flagellar beating. The connecting pieces are important building blocks for the sperm neck; however, the mechanism of connecting piece assembly is poorly understood. In the present study, we explored the role of septins in sperm motility and found that Sept12D197N knock-in (KI) mice produce acephalic and immotile spermatozoa.

Interacts With Autophagy to Offense or Defense.

Autophagy is an important component of the innate immune system in mammals. Low levels of basic autophagy are sustained in normal cells, to help with the clearance of aging organelles and misfolded proteins, thus maintaining their structural and functional stability. However, when cells are faced with challenges, such as starvation or pathogenic infection, their level of autophagy increases significantly.

FeO Nanoparticles Attenuated Infection in Chicken Liver Through Reactive Oxygen and Autophagy via PI3K/Akt/mTOR Signaling.

Recently nanomaterials have received substantial attention in biotechnology areas for their innovative properties in physical and chemical function. One of the most arrestive properties of nanomaterials that has been reported is their bacteriostatic activity. Our previous research found that FeO magnetic nanoparticles (FeO-NPs) could effectively reduce the viability of intracellular Enteritidis in chicken cells.

Effects of dietary N-carbamylglutamate supplementation on growth performance, tissue development and blood parameters of yellow-feather broilers.

The effects of N-carbamylglutamate (NCG) on the growth performance, tissue development, and blood parameters of broilers are unknown. In this study, 2 linked experiments were conducted to investigate the effects of 4 graded dietary levels and 3 dietary stages of NCG in a Chinese indigenous yellow-feather broiler breed during 2 growth phases: 1 to 18 d and 19 to 36 d. The dietary levels of NCG were 0.

Iron oxide nanozyme suppresses intracellular Enteritidis growth and alleviates infection .

Enteritidis ( Enteritidis) is a globally significant zoonotic foodborne pathogen which has led to large numbers of deaths in humans and caused economic losses in animal husbandry. Enteritidis invades host cells and survives within the cells, causing resistance to antibiotic treatment. Effective methods of elimination and eradication of intracellular Enteritidis are still very limited.

Regulation of septin phosphorylation: SEPT12 phosphorylation in sperm septin assembly.

The sperm annulus, a septin-based ring structure, is important for reproductive physiology. It is composed of SEPT12-based septin core complex followed by assembling as octameric filament. In clinical examinations, mutations of Septin12 result in male infertility, immotile sperm, as well as sperm with defective annuli.