Enhanced resilience to oxygen exposure and toxicity of chlorinated solvents in immobilized Dehalococcoides mccartyi.

Members of Dehalococcoides mccartyi (Dhc) are strictly anaerobic and play crucial roles in the restoration of many industrial sites impacted by chlorinated solvents, such as tetrachloroethene (PCE) and trichloroethene (TCE). In situ bioremediation with Dhc involves intricate procedures intended to minimize oxygen intrusion, and achieving optimal dechlorination performance in aquifers near the dense non-aqueous phase liquids source zone is challenging. Here, we respectively embedded Dhc strain 195 and the biomass of a Dhc-containing, PCE-dechlorinating consortium in poly(vinyl alcohol)-alginate hydrogel beads.

Sequential Reductive Dechlorination of Triclosan by Sediment Microbiota Harboring Organohalide-Respiring .

Aquatic ecosystems represent a prominent reservoir of xenobiotic compounds, including triclosan (TCS), a broad-spectrum biocide extensively used in pharmaceuticals and personal care products. As a biogeochemical hotspot, the potential of aquatic sediments for the degradation of TCS remains largely unexplored. Here, we demonstrated anaerobic biotransformation of TCS in a batch microcosm established with freshwater sediment.

sp. nov., a formate-oxidizing, organohalide-respiring bacterium isolated from grape pomace.

A strictly anaerobic, organohalide-respiring bacterium, designated strain GP, was characterized using a polyphasic approach. GP is Gram-stain-negative, non-spore-forming and non-motile. Cells are irregular cocci ranging between 0.

Organohalide Respiration with Diclofenac by .

Diclofenac (DCF) is a pharmaceutically active contaminant frequently found in aquatic ecosystems. The transformation pathways and microbiology involved in the biodegradation of DCF, particularly under anoxic conditions, remain poorly understood. Here, we demonstrated microbially mediated reductive dechlorination of DCF in anaerobic enrichment culture derived from contaminated river sediment.

Dehalogenation of Chlorinated Ethenes to Ethene by a Novel Isolate, " Dehalogenimonas etheniformans".

Dehalococcoides mccartyi strains harboring vinyl chloride (VC) reductive dehalogenase (RDase) genes are keystone bacteria for VC detoxification in groundwater aquifers, and bioremediation monitoring regimens focus on D. mccartyi biomarkers. We isolated a novel anaerobic bacterium, " Dehalogenimonas etheniformans" strain GP, capable of respiratory dechlorination of VC to ethene.

[Advances of using Dehalogenimonas in anaerobic degradation of chlorinated compounds and bioremediation of contaminated sites].

The genus Dehalogenimonas (Dhgm) is a recently discovered taxonomic group within the class Dehalococcoidia of the phylum Chloroflexi. To date, Dhgm consists of three formally described species including Dehalogenimonas lykanthroporepellens, Dehalogenimonas alkenigignens and Dehalogenimonas formicexedens. All isolates of these three Dhgm species are obligate organohalide-respiring bacteria.

Genome Sequence of " Dehalogenimonas etheniformans" Strain GP, a Vinyl Chloride-Respiring Anaerobe.

" Dehalogenimonas etheniformans" strain GP couples growth with the reductive dechlorination of vinyl chloride and several polychlorinated ethenes. The genome sequence comprises a circular 2.07-Mb chromosome with a G+C content of 51.