Mapping the distribution of perfluoroalkyl substances in zebrafishes by liquid extraction surface analysis mass spectrometry.

Investigation on the distribution of persistent organic pollutants (POPs) in aquatic organisms is of great importance for exploring the biological toxicity and health risks of environmental pollutants. In this study, a liquid extraction surface analysis mass spectrometry (LESA-MS) method was developed for rapid and in situ analysis of the spatial distribution of perfluoroalkyl substances (PFASs) in zebrafish. By combining the high-precision automated moving platform of LESA device and the high-resolution MS, quantitative analysis of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in zebrafish tissue section were easily achieved.

Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry Analysis Sputum for Non-Invasive Lung Adenocarcinoma Detection.

Purpose: Increased use of low-dose spiral computed tomography (LDCT: low-dose computed tomography) screening has contributed to more frequent incidental detection of peripheral lung nodules, part of them were adenocarcinoma, which need to be further evaluated to establish a definitive diagnosis. Here, our primary objective was to evaluate the ambient mass spectrometry (AMS) sputum analysis as a non-invasive lung adenocarcinoma (LAC) diagnosis solution.

Patients And Methods: Neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) and collision induced dissociation (CID) were used to detect sputum metabolites from 143 spontaneous sputum samples.

Comparative study of alterations in phospholipid profiles upon liver cancer in humans and mice.

Comparative studies of molecular alterations upon cancer between mice and humans are of great importance in order to determine the relevance of research involving mouse cancer models to the development of diagnostic and therapeutic approaches in clinical practice as well as for the mechanistic studies of pathology in humans. Herein, using molecular fingerprinting by internal extractive electrospray ionization mass spectrometry (iEESI-MS), we identified 50 differential signals in mouse liver tissue and 62 differential signals in human liver tissue that undergo significant intensity alterations (variable importance in the project (VIP) >1.0) upon liver cancer, out of which only 27 were common in both mouse and human tissues.

Lab-on-Membrane Platform Coupled with Paper Spray Ionization for Analysis of Prostate-Specific Antigen in Clinical Settings.

The analysis of protein antigens as biomarkers in clinical samples is particularly helpful for the early diagnosis of diseases. However, this is difficult to accomplish owing to the presence of the antigens in trace amounts as well as the complexity of the matrixes in clinical samples. In this study, a lab-on-membrane platform that can be combined with paper spray ionization mass spectrometry was developed for the high-throughput sensitive detection of the prostate-specific antigen (PSA).

Recent advances of ambient mass spectrometry imaging for biological tissues: A review.

Ambient mass spectrometry imaging (AMSI) is a molecular imaging technique developed in recent years for in situ and real time visualization of the distribution of chemical compounds in biological tissues without the need of labeling or staining. With the development for more than one decade, AMSI becomes a powerful molecular imaging technique in variousfields such as forensics, metabolomics, cancer diagnosis, and drug monitoring. In this review, we describe the recent advances of AMSI for imaging biological tissues in details.

Direct Analysis of Human Sputum for Differentiating Non-small Cell Lung Cancer by Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry.

Human sputum, a typical highly viscous biosample, was directly characterized at the molecular level using neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) without multi-step sample pretreatment, in an attempt to provide a method for constructing the pattern recognition of rapid diagnosis of lung cancer. Under the optimal experiment conditions, glucose, amino acids, phosphoric lipids and other typical analytes in the sputum sample could be used to conduct qualitative or quantitative (in arginine) analysis. More interestingly, the full scan mass spectra from 50 patients of non-small cell lung cancer, recording the mass spectral fingerprints of sputum samples, were differentiated from the control group (50 healthy individuals) through principal component analysis (PCA).

Quantitative Determination of Bulk Molecular Concentrations of β-Agonists in Pork Tissue Samples by Direct Internal Extractive Electrospray Ionization-Mass Spectrometry.

Rapid quantitative determination of bulk molecular concentration in solid samples without sample pretreatment is demonstrated using the internal extractive electrospray ionization-mass spectrometry (iEESI-MS) analysis of six β-agonists, including salbutamol (Sal), clenbuterol (Cle), ractopamine (Rac), terbutaline (Ter), tulobuterol (Tul), brombuterol (Bro), in pork tissue samples. Single sample analysis only required 1 min. The linear range of detection was about 0.

Metabolic Effects of Clenbuterol and Salbutamol on Pork Meat Studied Using Internal Extractive Electrospray Ionization Mass Spectrometry.

Direct mass spectrometry analysis of metabolic effects of clenbuterol and salbutamol on pork quality at the molecular level is incredibly beneficial for food regulations, public health and the development of new anti-obesity drugs. With internal extractive electrospray ionization mass spectrometry (iEESI-MS), nutrients including creatine, amino acids, L-carnitine, vitamin B, carnosine and phosphatidylcholines in pork tissue were identified, without sample pretreatment, using collision-induced dissociation (CID) experiments and by comparison with authentic compounds. Furthermore, normal pork samples were clearly differentiated from pork samples with clenbuterol and salbutamol via principal component analysis (PCA).