Microglial adenosine A receptor in the paraventricular thalamic nucleus regulates pain sensation and analgesic effects independent of opioid and cannabinoid receptors.

Introduction: The paraventricular thalamic nucleus (PVT) is recognized for its critical role in pain regulation, yet the precise molecular mechanisms involved remain poorly understood. Here, we demonstrated an essential role of the microglial adenosine A receptor (AR) in the PVT in regulating pain sensation and non-opioid analgesia.

Method And Results: Specifically, AR was predominantly expressed in ionized calcium binding adapter molecule 1 (Iba1)-positive microglia cells within the PVT, with expression levels remaining unchanged in mice experiencing persistent inflammatory pain induced by complete Freund's adjuvant (CFA).

Exogenous Amyloid Fibrils Can Cause Significant Upregulation of Neurodegenerative Disease Proteins.

Neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease, are associated with the formation of amyloid fibrils. In familial cases, the mutant causative genes accentuate disease progression through overexpression or misfolding of amyloidogenic proteins. Besides, considerable amyloidosis cases arise from external factors, but their origin and mechanisms are not yet fully understood.

A structural rationale for reversible vs irreversible amyloid fibril formation from a single protein.

Reversible and irreversible amyloids are two diverging cases of protein (mis)folding associated with the cross-β motif in the protein folding and aggregation energy landscape. Yet, the molecular origins responsible for the formation of reversible vs irreversible amyloids have remained unknown. Here we provide evidence at the atomic level of distinct folding motifs for irreversible and reversible amyloids derived from a single protein sequence: human lysozyme.

Dynamic phase-differencing profilometry with number-theoretical phase unwrapping and interleaved projection.

High-speed 3D measurement is receiving increasing attention. However, simultaneously achieving high computational efficiency, algorithmic robustness, and reconstructing ratio is challenging. Therefore, a dynamic phase-differencing profilometry (DPDP) is proposed.

An evolutionarily conserved mechanism controls reversible amyloids of pyruvate kinase via pH-sensing regions.

Amyloids are known as irreversible aggregates associated with neurodegenerative diseases. However, recent evidence shows that a subset of amyloids can form reversibly and fulfill essential cellular functions. Yet, the molecular mechanisms regulating functional amyloids and distinguishing them from pathological aggregates remain unclear.

A multihole nozzle controls recrystallization of high-moisture extruded maize starches: Effect of cooling die temperature.

Hot extrusion is utilized for starch modification due to its high mechanical input and product output. Amylose recrystallization commences and primarily depends on intermolecular interactions after conventional extrusion. Hence, the design of a new component based on the existed extrusion system was aimed at facilitating molecular aggregation, potentially accelerating starch recrystallization.

Orthogonal Spatial Binary Coding Method for High-Speed 3D Measurement.

Temporal phase unwrapping based on single auxiliary binary coded pattern has been proven to be effective for high-speed 3D measurement. However, in traditional spatial binary coding, it often leads to an imbalance between the number of periodic divisions and codewords. To meet this challenge, a large codewords orthogonal spatial binary coding method is proposed in this paper.

NSD family proteins: Rising stars as therapeutic targets.

Epigenetic modifications, including DNA methylation and histone post-translational modifications, intricately regulate gene expression patterns by influencing DNA accessibility and chromatin structure in higher organisms. These modifications are heritable, are independent of primary DNA sequences, undergo dynamic changes during development and differentiation, and are frequently disrupted in human diseases. The reversibility of epigenetic modifications makes them promising targets for therapeutic intervention and drugs targeting epigenetic regulators (e.

Carboxyl graphene modified PEDOT:PSS organic electrochemical transistor for in situ detection of cancer cell morphology.

Circulating tumor cells in human peripheral blood play an important role in cancer metastasis. In addition to the size-based and antibody-based capture and separation of cancer cells, their electrical characterization is important for rare cell detection, which can prove fatal in point-of-care testing. Herein, an organic electrochemical transistor (OECT) biosensor made of solution-gated carboxyl graphene mixed with PEDOT:PSS for the detection of cancer cells is reported.

High-moisture extrusion of curdlan: Texture and structure.

High-moisture extrusion is a promising thermomechanical technology extensively employed in manufacturing fibrous meat analogues from plant-based proteins, garnering considerable research attention. However, polysaccharide-based extrusion has been rarely explored. The present study investigates the effects of varying extruder barrel temperatures (130 °C-200 °C) on the texture and structure of curdlan extrudates, and highlights the formation mechanism.

Fabrication of PEDOT:PSS-based solution gated organic electrochemical transistor array for cancer cells detection.

Organic electrochemical transistor (OECT) was applied in chemical and biological sensing. In this work, we developed a simple and repeatable method to fabricate OECT array, which had been successfully used to detect cancer cells. PEDPT:PSS conductive film between source and drain electrodes were patterned through photolithography, which can achieve uniform devices with same electrical characterization.

Absolute phase retrieval based on fringe amplitude encoding without any additional auxiliary pattern.

An absolute phase retrieval method based on fringe amplitude encoding is proposed. Different from the conventional intensity coding methods which are based on time division multiplexing with multiple additional auxiliary patterns, the proposed fringe order encoding strategy is codeword overlapping interaction based on space division multiplexing. It just directly encodes different fringe amplitudes for different periods in corresponding sinusoidal phase-shifting patterns to generate space division multiplexing composite sinusoidal phase-shifting patterns and quantifies the fringe amplitudes into four levels as encoding strategy, so it can retrieve absolute phase without any additional auxiliary patterns.

Enhanced Fourier-Hilbert-transform suppression for saturation-induced phase error in phase-shifting profilometry.

Intensity saturation tends to induce severe errors in high dynamic range three-dimensional measurements using structured-light techniques. This paper presents an enhanced Fourier-Hilbert-transform (EFHT) method to suppress the saturation-induced phase error in phase-shifting profilometry, by considering three types of residual errors: nonuniform-reflectivity error, phase-shift error, and fringe-edge error. Background normalization is first applied to the saturated fringe patterns to suppress the effect of the nonuniform reflectivity.

Saturation-Induced Phase Error Compensation Method Using Complementary Phase.

Intensity saturation can induce phase error and, thus, measurement error in fringe projection profilometry. To reduce saturation-induced phase errors, a compensation method is developed. The mathematical model of saturation-induced phase errors is analyzed for -step phase-shifting profilometry, and the phase error is approximately -folder of the frequency of the projected fringe.

The establishment of city commercial banks and China's green economy development.

Promoting sustainable economic development has been pursued by all countries, and achieving green economic development is crucial to sustainable economic development. This study uses the non-radial direction distance function (NDDF) method to calculate the level of development of the green economy in Chinese cities during 2003-2014. Next, it uses the establishment of China's city commercial banks as an exogenous policy shock to build a staggered difference-in-differences model to empirically test the impact of the establishment of city commercial banks on green economy development.

Ultra-fast 3D imaging by a big codewords space division multiplexing binary coding.

Patternless binary coding strategies have been a challenge for ultra-fast 3D imaging with structured light. This Letter proposes a big codewords space division multiplexing binary coding method. From the third to the multiple order, a special spatial binary coding instead of the Gray code is created for the first time, to the best of our knowledge, to achieve an ultra-wide unambiguous range with only one auxiliary pattern.

Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture.

A 3D network capture substrate based on poly(lactic-co-glycolic acid) (PLGA) nanofibers was studied and successfully used for high-efficiency cancer cell capture. The arc-shaped glass micropillars were prepared by chemical wet etching and soft lithography. PLGA nanofibers were coupled with micropillars by electrospinning.

Temporal phase unwrapping based on unequal phase-shifting code.

In fringe projection profilometry (FPP) based on temporal phase unwrapping (TPU), reducing the number of projecting patterns has become one of the most important works in recent years. To remove the 2π ambiguity independently, this paper proposes a TPU method based on unequal phase-shifting code. Wrapped phase is still calculated from N-step conventional phase-shifting patterns with equal phase-shifting amount to guarantee the measuring accuracy.

Dynamic computer-generated moiré profilometry based on high-density binary coding.

A dynamic computer-generated moiré profilometry based on high-density binary coding is proposed. For making full use of the maximum refresh rate and the maximum resolution of the digital light projector (DLP), the binary coded fringe is used to replace the conventional 256-gray-scale sinusoidal fringe, which can increase the refresh rate from the traditional 120 Hz to more than 1 kHz and meet the needs of dynamic measurement from the source. To realize the minimum equivalent wavelength and obtain the purest calculated moiré fringe, a minimum four-pixel period high-density binary fringe that satisfies the sampling theorem is designed for the DLP.

Interfacial Electrostatic Self-Assembly of Amyloid Fibrils into Multifunctional Protein Films.

Amyloid fibrils have generated steadily increasing traction in the development of natural and artificial materials. However, it remains a challenge to construct bulk amyloid films directly from amyloid fibrils due to their intrinsic brittleness. Here, a facile and general methodology to fabricate macroscopic and tunable amyloid films via fast electrostatic self-assembly of amyloid fibrils at the air-water interface is introduced.

Relationship between coliphage and Enterococcus at southern California beaches and implications for beach water quality management.

Coliphage have been suggested as an alternative to fecal indicator bacteria for assessing recreational beach water quality, but it is unclear how frequently and at what types of beaches coliphage produces a different management outcome. Here we conducted side-by-side sampling of male-specific and somatic coliphage by the new EPA dead-end hollow fiber ultrafiltration (D-HFUF-SAL) method and Enterococcus at southern California beaches over two years. When samples were combined for all beach sites, somatic and male-specific coliphage both correlated with Enterococcus.

Nonlinear error full-field compensation method for phase measuring profilometry.

Phase measuring profilometry (PMP) has the highest measuring accuracy among structured light projection-based three-dimensional (3D) sensing methods. Due to their low-cost and high-resolution features, commercial projectors are extensively used in PMP, but they are all designed with a gamma effect purpose that considers the characteristics of human vision. Affected by the gamma effect, a set of phase-shifting sinusoidal deformed patterns captured in PMP may contain high-order harmonics which lead to nonlinear phase errors.

Stormwater biofilter response to high nitrogen loading under transient flow conditions: Ammonium and nitrate fates, and nitrous oxide emissions.

Nitrogen (N) in urban runoff is often treated with green infrastructure including biofilters. However, N fates across biofilters are insufficiently understood because prior studies emphasize low N loading under laboratory conditions, or use "steady-state" flow regimes over short time scales. Here, we tested field scale biofilter N fates during simulated storms delivering realistic transient flows with high N loading.