Correction: Open-flow microperfusion combined with mass spectrometry for liver lipidomic analysis.

Correction for 'Open-flow microperfusion combined with mass spectrometry for liver lipidomic analysis' by Tuo Li , , 2021, , 1915-1923, https://doi.org/10.1039/D0AN02189J.

G-quadruplex inducer/stabilizer pyridostatin targets SUB1 to promote cytotoxicity of a transplatinum complex.

Pyridostatin (PDS) is a well-known G-quadruplex (G4) inducer and stabilizer, yet its target genes have remained unclear. Herein, applying MS proteomics strategy, we revealed PDS significantly downregulated 22 proteins but upregulated 16 proteins in HeLa cancer cells, of which the genes both contain a number of G4 potential sequences, implying that PDS regulation on gene expression is far more complicated than inducing/stabilizing G4 structures. The PDS-downregulated proteins consequently upregulated 6 proteins to activate cyclin and cell cycle regulation, suggesting that PDS itself is not a potential anticancer agent, at least toward HeLa cancer cells.

Baicalin Targets HSP70/90 to Regulate PKR/PI3K/AKT/eNOS Signaling Pathways.

Baicalin is a major active ingredient of traditional Chinese medicine , and has been shown to have antiviral, anti-inflammatory, and antitumor activities. However, the protein targets of baicalin have remained unclear. Herein, a chemical proteomics strategy was developed by combining baicalin-functionalized magnetic nanoparticles (BCL-N@MNPs) and quantitative mass spectrometry to identify the target proteins of baicalin.

Open-flow microperfusion combined with mass spectrometry for in vivo liver lipidomic analysis.

At present, conventional microdialysis (MD) techniques cannot efficiently sample lipids in vivo, possibly due to the high mass transfer resistance and/or the serious adsorption of lipids onto the semi-permeable membrane of a MD probe. The in vivo monitoring of lipids could be of great significance for the study of disease development and mechanisms. In this work, an open-flow microperfusion (OFM) probe was fabricated, and the conditions for sampling lipids via OFM were optimized.

Visualization of Proteins in Single Cells by Time-of-Flight-Secondary Ion Mass Spectrometry Coupled with Genetically Encoded Chemical Tags.

visualization of proteins of interest in single cells is attractive in cell biology, molecular biology, and biomedicine fields. Time-of-flight-secondary ion mass spectrometry (ToF-SIMS) is a powerful tool for imaging small organic molecules in single cells, yet difficult to image biomacromolecules such as proteins and DNA. Herein, a universal strategy is reported to image specific proteins in single cells by ToF-SIMS following genetic incorporation of fluorine-containing unnatural amino acids as a chemical tag into the proteins a genetic code expansion technique.

Uptake and Transformation of Silver Nanoparticles and Ions by Rice Plants Revealed by Dual Stable Isotope Tracing.

Knowledge on the uptake and transformation of silver nanoparticles (AgNPs) and Ag ions by organisms is critical for understanding their toxicity. Herein, the differential uptake, transformation, and translocation of AgNPs and Ag ions in hydroponic rice ( Oryza sativa L.) is assessed in modified Hewitt (with Cl ions, HS(Cl)) and Hogland solutions (without Cl ions, HS) using dual stable isotope tracing (AgNO and AgNPs).

Enantioselective degradation of alpha-cypermethrin and detection of its metabolites in bullfrog (rana catesbeiana).

Bullfrog, as a kind of amphibians, can be easily exposed to varied pollutants in the environment for the reason of its habitats and highly permeable skin. We investigated the degradation kinetics and residues of α-cypermethrin in bullfrog by two different methods of administration for the environmental monitoring the behavior of one of the most used pesticides in the amphibians. The oral administration and water exposure of α-cypermethrin on bullfrog was studied in this work.